Molecular Diagnosis of Cutaneous Diseases

Karan, Kalpita R; Babb-Tarbox, Michelle; Aboutalebi, Sina; Rády, Peter L.; Shipley, Gregory L.; Dao, Dat D.; Tyring, Stephen K.

doi:10.1001/archderm.141.2.225

Cited by 21 publications

(15 citation statements)

References 162 publications

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“…Recently, the incidence of NHL has increased year by year, with an annual increasing rate of 8-10% throughout the world (Thieblemont et al, 2008), of extranodal NHL also continues to rise and accounts for 25-40% of the same period's NHL cases in Western countries (Zucca, 2008). The detection of the TCRγ gene rearrangement sequence, namely the malignant cloning of T cells, would be useful in the molecular staging of the disease (Sra et al, 2005), so that the classifications of T and B cell lymphoma would be much clearer.…”

Section: Discussionmentioning

confidence: 99%

Significance of detecting IgH and TCRγ gene rearrangements in patients with hemopoietic maligancies by real-time quantitative PCR

Yao¹,

Rh²,

B³

et al. 2015

Genet. Mol. Res.

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Section: Discussionmentioning

confidence: 99%

Significance of detecting IgH and TCRγ gene rearrangements in patients with hemopoietic maligancies by real-time quantitative PCR

Yao¹,

Rh²,

B³

et al. 2015

Genet. Mol. Res.

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“…Future studies are indicated to follow-up on the transcriptome defined for the various human KC cultures by exploring the transcriptome of normal human skin under both physiological and pathological conditions. The coordinated gene expression profile derived from such in vivo studies is likely to shed new insights into the molecular mechanisms regulating tissue homeostasis, as well as identifying novel targets for therapeutic consideration in skin diseases with altered barrier function and/ or for aging skin related abnormalities [Sra et al, 2005].…”

Section: Discussionmentioning

confidence: 99%

Defining the transcriptome of accelerated and replicatively senescent keratinocytes reveals links to differentiation, interferon signaling, and Notch related pathways

Perera

Koo

Bennett

et al. 2006

J of Cellular Biochemistry

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Epidermal keratinocytes (KCs) undergo highly orchestrated morphological and molecular changes during transition from proliferative compartment into growth arrested early and late differentiation layers, prior to dying in outermost cornified layers of normal skin. Creation of stratum corneum is vital to barrier function protecting against infection. Transcriptional events in KCs regulating complex processes of differentiation and host defense required to maintain constant epidermal thickness and resistance to infection in either young or aged skin are largely unknown. Furthermore, as terminal differentiation is characterized by irreversible loss of replicative potential culminating in dead layers at the skin surface, this process may be viewed as a form of senescence. However, a complete transcriptional profile of senescent (SN) human KCs has not been previously defined to permit delineation of molecular boundaries involving differentiation and senescence. To fill this void, we utilized global transcriptional analysis of KCs maintained in vitro as either cultures of proliferating (PR) cells, early and late confluent (LC) (accelerated senescence) cultures, or KCs undergoing replicative senescence. Global gene expression profiling revealed early confluent (EC) KCs were somewhat similar to PR KCs, while prominent differences were evident when compared to LC KCs; which were also distinct from replicatively SN KCs. While confluent KCs have in common several genes regulating differentiation with replicatively SN KCs, the latter cells expressed elevated levels of genes involved in interferon signaling and inflammatory pathways. These results provide new insights into cell autonomous transcriptional-based programs operative within KCs contributing to replicative senescence, with partial sharing of genes involved in differentiation. In addition, regulation of KC senescence may involve participation of interferon signaling pathways derived from the important role of KCs in protecting skin from infection. Integrating all of the transcriptional data revealed a key role for Notch receptor mediated signaling in the confluency induced differentiation phenotype using this model system.

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“…Für die Charakterisierung kutaner Lymphome stehen über 50 distinkte Antikörper zur Verfügung, die je nach Verdacht in unterschiedlichen Sets angewendet eine Zuordnung in die WHO-EORTC-Klassifikation erleichtern [8,11] (RT-PCR) auch für RNA-Fragmente. Eine quantitative Beurteilung war ab 1996 mit Einführung der Realtime-PCR mög-lich, und das über eine dynamische Breite von 7 Log-Stufen [15,39]. Zu der Popularität der Methode haben zudem ihre relativ schnelle Durchführbarkeit, leichte Standardisierbarkeit und kosteneffektive Nutzbarkeit beigetragen.…”

Section: Moderne Molekulare Methodenunclassified

“…Tab. 4, [39,40]). Die Verfahren sind mittlerweile so standardisiert und reproduzierbar, dass die PCR-Analyse unter anderem für Mycobacterium tuberculosis, Herpes simplex 1 und 2, HIV und Hepatitis C von öffentlichen Kontrollgremien wie der FDA als Diagnostikmethode zugelassen worden ist.…”

Section: Moderne Molekulare Methodenunclassified

Molekulargenetik in der dermatologischen Diagnostik

Braun‐Falco

Ruzicka

2012

Hautarzt

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Modern molecular techniques have tremendously expanded our knowledge about the biologic processes in healthy individuals as well as our understanding about the pathologic events in an increasing number of dermatological diseases. These technologies initially came from basic molecular biology and genetic research but have become firmly anchored in clinical diagnosis approaches. Included in this group are immunohistochemistry (IHC), polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), chromogen in situ hybridization (CISH), comparative genomic hybridization (CGH), and microarray technology. IHC and PCR already belong to the armamentarium for routine daily diagnostics due to their high degree of standardization and reproducibility, ease of use and relatively low costs. Others like FISH and CISH are currently employed for specific indications in a growing number of larger laboratories, whereas CGH and microarray technology still remain in the hands of a few highly specialized laboratories. These new ancillary methods will help to improve diagnostic accuracy particularly in cases in which conventional histopathology is ambiguous. In addition, they will provide new and important information concerning the prognosis, progression and response rate to therapies in several particular malignant diseases.

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Molecular Diagnosis of Cutaneous Diseases

Cited by 21 publications

References 162 publications

Significance of detecting IgH and TCRγ gene rearrangements in patients with hemopoietic maligancies by real-time quantitative PCR

Significance of detecting IgH and TCRγ gene rearrangements in patients with hemopoietic maligancies by real-time quantitative PCR

Defining the transcriptome of accelerated and replicatively senescent keratinocytes reveals links to differentiation, interferon signaling, and Notch related pathways

Molekulargenetik in der dermatologischen Diagnostik

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