Molecular diagnosis of human papillomavirus (HPV) infections

Molijn, Anco; Kleter, Bernhard; Quint, Wim; Doorn, Leen–Jan van

doi:10.1016/j.jcv.2004.12.004

Cited by 256 publications

(276 citation statements)

References 71 publications

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“…The diagnosis of HPV infection is made by histopathology of lesions 11 or detection of viral DNA in infected cells. 12 Hybridization techniques and polymerase chain reaction (PCR) are methods used for HPV detection.…”

Section: Techniques For Detection Of Hpvmentioning

confidence: 99%

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Infecção pelo papilomavírus humano: etiopatogenia, biologia molecular e manifestações clínicas

Leto¹,

Júnior²,

Porro

et al. 2011

An. Bras. Dermatol.

113

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Human papillomavirus (HPV) is a DNA virus that presents tropism for epithelial cells, causing infections of the skin and mucous membranes. Replication of HPV occurs in the nuclei of squamous cells and its life cycle is directly related to the differentiation program of the host cell. To date, nearly 100 different types of HPV have been characterized and there is a large number of other types that have not been sequenced yet. Besides being responsible for benign lesions of the skin and mucous membranes, HPV is also involved in the development of various mucocutaneous tumors: Bowen's disease, non-melanoma skin cancers and genital carcinomas. This review discusses the characteristics of HPV, malignant and benign mucous and skin manifestations caused by HPV, besides the main methods of detection and typing of the virus. Keywords: DNA Viruses; Papillomavirus Infections; Warts Resumo: O papilomavírus humano (HPV) é um vírus DNA que apresenta tropismo por células epiteliais, causando infecções na pele e nas mucosas. A replicação do HPV ocorre no núcleo das células escamosas e o seu ciclo de vida é diretamente relacionado ao programa de diferenciação da célula hospedeira. Até o momento, foram completamente caracterizados cerca de 100 tipos diferentes de HPVs e há um grande número adicional de tipos ainda não sequenciados. Além de ser o responsável por lesões benignas de pele e mucosas, o HPV também está envolvido no desenvolvimento de diversos tumores cutaneomucosos: doença de Bowen, cânceres de pele não melanoma e carcinomas genitais. Esta revisão aborda as características do HPV, quadros cutâneos e mucosos benignos e malignos causados por ele e os principais métodos empregados em sua detecção e tipagem.

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Section: Techniques For Detection Of Hpvmentioning

confidence: 99%

“…The techniques most often used for typing of the DNA amplified by means of PCR are: Southern blot, dot blot, reverse hybridization, restriction enzyme digestion, and sequencing. 12 …”

Section: Techniques For Detection Of Hpvmentioning

confidence: 99%

Infecção pelo papilomavírus humano: etiopatogenia, biologia molecular e manifestações clínicas

Leto¹,

Júnior²,

Porro

et al. 2011

An. Bras. Dermatol.

113

View full text Add to dashboard Cite

show abstract

“…3 Since human papillomavirus (HPV) has been established as a well-recognized etiologic agent of cervical neoplasia, HPV DNA testing is a promising alternative or complementary test to improve the efficacy of cervical screening and probably cost-effective, depending on screening interval. 4,5 Cervical cancer screening programs integrating HPV testing have been advocated in United Kingdom, United States, South Africa, Thailand and Germany. [6][7][8][9][10] Hybrid Capture assay (HCII) (Digene Corporation, Gaithersburg, MD) is the most widely used method of HPV DNA testing.…”

mentioning

confidence: 99%

Cervical cancer screening program integrating Pap smear and HPV DNA testing: A population‐based study

Chao

Hsu

Lai

et al. 2008

Intl Journal of Cancer

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We conducted a population-based cohort study to evaluate the complementary value of HPV testing to Papanicolaou (Pap) smear and the prevalence and genotype distribution of HPV in Taiwan. In this report, we described the design of the whole study and analyzed the cross-sectional results. Female residents (age 30 years) of Taoyuan, Taiwan were invited. After signing informed consent, every participant had a Pap smear and a HPV testing. Patients with Pap atypical squamous cell of undetermined significance (Group I) or those with HPV-positive but normal cytology (Group II) were referred for a colposcopic examination. A total of 10,014 women were eligible. The overall HPV prevalence was 10.8% (95% confidence interval 10.5%-11.4%) in the study population. A total of 37 types of HPV were identified and the leading three were HPV-52, -18 and -58. There was a significant positive correlation of HPV prevalence with older age, postmenopausal status, current-user of oral contraceptives and never-user of hormone replacement therapy. Past users of oral contraceptives and never users of Pap were associated with higher risk of abnormal Pap, while age 40-49 strata had lower risk. Fifty-nine cases of cervical intraepithelial neoplasia (CIN) 2 from Group I and additional 11 from Group II were identified. The improvement of sensitivity with additional HPV testing was 15.3%. Besides, no specific subgroup was found to most benefit from the combined strategy. The value of adding HPV test to conventional Pap smear has to be evaluated after longer-term follow-up of this population-based cohort. ' 2008 Wiley-Liss, Inc.Key words: cervical cancer screening; Pap smear; human papillomavirus; polymerase chain reaction (PCR); prevalence Cervical cancer continues to be one of the leading female genital cancers worldwide. 1 The cervical cancer burden in Taiwan is still heavy with the incidence of invasive cancer being 24.5/ 100,000, which ranked the fifth place of cancer death in women. 2 Traditionally, primary screening of cancer precursors remains based on cytological classification of cervical smears. Conventional Papanicolaou (Pap) test or monolayer cytology provided sensitivity rates ranged from 30% to 87% and specificity rates from 86% to 100%. 3 Since human papillomavirus (HPV) has been established as a well-recognized etiologic agent of cervical neoplasia, HPV DNA testing is a promising alternative or complementary test to improve the efficacy of cervical screening and probably cost-effective, depending on screening interval. 4,5 Cervical cancer screening programs integrating HPV testing have been advocated in United Kingdom, United States, South Africa, Thailand and Germany. [6][7][8][9][10] Hybrid Capture assay (HCII) (Digene Corporation, Gaithersburg, MD) is the most widely used method of HPV DNA testing. HCII utilizes liquid hybridization of RNA probes against HPV DNA followed by signal amplification, has been validated for its reproducibility. 11 Methods using PCR is more sensitive and quality assays were generally consistent for...

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“…An HPV infection is diagnosed mainly using molecular biological methods because culturing and in vitro propagation of viruses are impractical and serological methods are insufficiently sensitive [26]. These molecular methods include DNA-based assays (e.g., PCR, in situ hybridization, reverse line plot, and the Hybrid Capture 2 [HC2] DNA assay) and RNAbased assays (e.g., mRNA gene expression and protein assays).…”

Section: Hpv Screening Methodsmentioning

confidence: 99%

Current studies on human papillomavirus in Saudi Arabia

Alhamlan

Al‐Qahtani

Al-Ahdal

2015

J Infect Dev Ctries

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Human papillomavirus (HPV) infection is a significant etiological factor and an important prognosticator in cervical cancer. Indeed, researchers worldwide have confirmed these roles for high-risk HVPs in over 70% of cervical cancer cases. According to the World Health Organization, approximately 561,200 new cancer cases (5.2% of all new cancers) are attributed to HPV infection. Over 120 types of HPV are classified further as either low-risk HPV (LR-HPV) or high-risk HPV (HR-HPV) based on their oncological potential of transforming cells. The LR-HPV types cause benign hyperproliferative lesions (i.e. genital warts) while the HR-HPV types are strongly associated with premalignant and malignant cervical lesions. Data on the prevalence of HPV, survival of infected patients, and mortality rate are scarce in Saudi Arabia. The unsubstantiated assumption of a low prevalence of HPV in Saudi Arabia has contributed to limiting HPV research in this conservative country. Therefore, the goal of this review is to shed light on the current HPV research being conducted and the prevalence of HPV in Saudi Arabia.

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Molecular diagnosis of human papillomavirus (HPV) infections

Cited by 256 publications

References 71 publications

Infecção pelo papilomavírus humano: etiopatogenia, biologia molecular e manifestações clínicas

Infecção pelo papilomavírus humano: etiopatogenia, biologia molecular e manifestações clínicas

Cervical cancer screening program integrating Pap smear and HPV DNA testing: A population‐based study

Current studies on human papillomavirus in Saudi Arabia

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