Molecular Diagnosis of Mycoplasma Species Infection in Camels Using Semi-nested PCR

Molecular detection of Mycoplasma spp. from camel’s milk

“…Mycoplasma spp. was a cell wall deficient prokaryotes bacteria classified under the class Mollicutes, and its definitive as the smallest organism capable of self-replicating (14). Most of the Mycoplasma infections in camels remain undiagnosed, partly because of the scarcity of camel's Mycoplasma studies (23) and because the Mycoplasma classical diagnosis method is complex and takes a long time.…”

“…Moreover, the Mycoplasma serological tests could give false results because of the cross-reactivity of Mycoplasma spp. with other closely related bacterial species (14,24,25). In recent decades, molecular methods have been used to diagnose Mycoplasma infection.…”

“…In recent decades, molecular methods have been used to diagnose Mycoplasma infection. The introduction of molecular diagnoses using the polymerase chain reaction (PCR) method with a specific primer provides a rapid and accurate tool to detect and identify Mycoplasma infections (14,15,(26)(27)(28)(29). The previous studies reported that many species of Mycoplasma were associated with mammary gland infection in cows, including M. Bovis, M. californium, M. bovigenitalium, M. alkalescens, and M. canadense (30).…”

“…Camel's milk considers an exceptional nutritional food because of its high nutritional and therapeutic uses (9)(10)(11)(12)(13). Although camels consider more resistant to many diseases that occur in other animals (14), many diseases can affect camels and lead to crucial economic loss (15). In many countries, including Iraq, camel's milk production was affected by mammary gland infection, also known as mastitis (16).…”