Molecular Diagnosis of Placental Hydatidiform Mole: Innovation and Outcomes

Colgan, Terence J.; Noor, Abdul; Nanji, Shabin; Chang, Martin C.; Kolomietz, Elena

doi:10.1016/j.jogc.2017.05.023

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…explained how ancillary techniques target the unique genetic features of HMs to establish diagnostic truth and highlighted the issue of diagnostic reproducibility. [89] They explained the importance of diagnostic accuracy and illustrated the use of p57 immunohistochemistry and polymerase chain reaction-based DNA genotyping for diagnosis. Genotyping, which compares villous and decidual DNA patterns to determine the parental source and ratios of polymorphic alleles, distinguishes purely androgenetic complete HMs from diandric triploid partial HMs, and both of these from biparental nonmolar specimens.…”

Section: Discussionmentioning

confidence: 99%

“…They opined that an algorithmic approach using these techniques should be advocated for diagnosis of these cases. [89] In a recent study, differential expression of Twist1, Ki-67, and E-cadherin was analyzed by Moussa et al ., in gestational products from 55 cases of CM, PM, and HA using immunohistochemistry. [10] In their study, they found Twist1 expression was a highly reliable marker for the diagnosis of CM, whereas combined Ki-67 and E-cadherin immunoreactivity could distinguish PHM from nonmolar pregnancies.…”

Section: Discussionmentioning

confidence: 99%

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Expression of p57 immunomarker in the classification and differential diagnosis of partial and complete hydatidiform moles

et al. 2019

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BACKGROUND: Hydatidiform mole (HM) is characterized histologically by cystic swelling of the chorionic villi, accompanied by variable trophoblastic proliferation. The most important reason for the correct recognition of moles is that they are associated with an increased risk of persistent trophoblastic disease (invasive mole) or choriocarcinoma. AIMS AND OBJECTIVES: The aim of the study was to determine whether there is any role of p57 in differentiating partial and complete moles by immunohistochemical staining. MATERIALS AND METHODS: A prospective observational study was undertaken in which 40 cases of molar pregnancy included over a period of 2 years. Detailed clinical and family histories were obtained from each patient. Histopathological examination followed by immunohistochemical study with p57 done in each case. Ultrasonography findings and serial titers of serum beta-human chorionic gonadotropin were noted whenever necessary. RESULTS: Among the forty cases included, 25 (62%) had complete molar (CM) pregnancy, whereas the rest 15 (38%) had partial mole (PM). Both CM and PM were more pronounced in the age group of 20–25 years (44% and 60%, respectively), and among nulliparous women (68% and 70% respectively), 17 (42.5%) mothers had a prior history of abortion. In the histologically unequivocal cases of complete mole, 96% (24 of 25) did not express p57 and a single case was focal positive. In contrast, it was strongly and continuously expressed in both villous cytotrophoblast and stromal cells in all cases of PM (15 of 15). CONCLUSION: p57 immunomarker is very helpful to diagnose and differentiate complete and partial HM.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression of p57 immunomarker in the classification and differential diagnosis of partial and complete hydatidiform moles

et al. 2019

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“…A large study conducted in a Canadian lab analyzed over 400 cases of suspected placental mole resulting in 113 complete moles, 141 partial moles and 189 nonmolar abortus. This group concluded that (a) partial mole is about twice as common as complete mole; (b) that molecular techniques are essential for accurate diagnosis in many suspected placental molar cases and (c) they identified aneuploidy in about one-fifth of non-molar cases [8].…”

Section: Discussionmentioning

confidence: 99%

“…It has to be pointed out that not all the pathology studies were performed by a perinatal pathologist, because sometimes they are carried out by non-experienced pathologists. Histopathologic diagnosis of placental mole is imprecise, even when carried out by experts [8]. It has been described that triploidy correlates with the presence of three or four major diagnostic criteria of partial mole: (a) two populations of villi; (b) enlarged, irregular, dysmorphic villi (with trophoblast inclusions); (c) enlarged, cavitated villi (≥3-4 mm); and (d) syncytiotrophoblast hyperplasia/atypia, commonly in addition to histologic evidence of fetal development [12,13].…”

Section: Discussionmentioning

confidence: 99%

“…The use of QF-PCR in the diagnosis of molar pregnancies has been previously reported by large labs [8], but we are not aware of clinical studies assessing its feasibility in clinical practice. In the present study, we investigated the contribution of both, QF-PCR and pathology studies, in the diagnosis of diandric triploidies/partial moles.…”

Section: Introductionmentioning

confidence: 99%

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The Contribution of QF-PCR and Pathology Studies in the Diagnosis of Diandric Triploidy/Partial Mole

et al. 2021

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Objective: the aim of our study was to assess the contribution of quantitative fluorescent polymerase chain reaction (QF-PCR) and pathology studies in the diagnosis of diandric triploidies/partial hydatidiform moles. Methods: this study included all fet al triploidies diagnosed by QF-PCR in chorionic villi or amniotic fluid in the 2 centers of BCNatal in which a maternal saliva sample was used to establish its parental origin. Pathology studies were performed in products of conception and concordance between a partial hydatidiform mole diagnosis and the finding of a diandric triploidy was assessed. Results: among 46 fetal triploidies, found in 13 ongoing pregnancies and in 33 miscarriages, there were 26 (56%) diandric triploidies. Concordant molecular (diandric triploidy) and pathology results (partial mole) were achieved in 14 cases (54%), while in 6 cases (23%) pathology studies were normal, and in the remaining 6 cases (23%) pathology studies could not be performed because miscarriage was managed medically. Conclusions: diandric triploidy is associated with partial hydatidiform mole and its diagnosis is crucial to prevent the development of persistent trophoblastic disease. QF-PCR analysis in chorionic villi or amniotic fluid provides a more accurate diagnosis of the parental origin of triploidy than the classical pathology studies.

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Pathology of gestational trophoblastic disease (GTD)

Kaur

2021

Best Practice & Research Clinical Obstetrics & Gynaecol

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Molecular Diagnosis of Placental Hydatidiform Mole: Innovation and Outcomes

Cited by 5 publications

References 14 publications

Expression of p57 immunomarker in the classification and differential diagnosis of partial and complete hydatidiform moles

Expression of p57 immunomarker in the classification and differential diagnosis of partial and complete hydatidiform moles

The Contribution of QF-PCR and Pathology Studies in the Diagnosis of Diandric Triploidy/Partial Mole

Pathology of gestational trophoblastic disease (GTD)

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