Molecular diagnosis of potentially human pathogenic Enterocytozoon bieneusi and Encephalitozoon species in exotic birds in Southwestern Iran

Tavalla, Mehdi; Mardani-Kateki, Masoumeh; Abdizadeh, Rahman; Soltani, Shahrzad; Saki, Jasem

doi:10.1016/j.jiph.2017.07.028

Cited by 27 publications

(12 citation statements)

References 30 publications

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“…In our study, we detected, for the first time, the prevalence (6.59%) of E. bieneusi in peafowl, which was similar to some studies performed in other birds (Bart et al, 2008; Lallo et al, 2012; Pirestani et al, 2013). In contrast to our result, much higher prevalence was found in studies conducted by Li et al (2014) in chicken, Lobo et al (2006) in pigeon and Tavalla et al (2018) in exotic birds. Lower infection rates were identified in pigeon and Brazilian captive birds investigated by Slodkowica-Kowalskaet et al (2013) and da Cunha et al (2017) respectively.…”

Section: Discussioncontrasting

confidence: 99%

First report of Enterocytozoon bieneusi and Cryptosporidium spp. in peafowl (Pavo cristatus) in China

Feng¹,

Han²,

Luo³

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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Enterocytozoon bieneusi and Cryptosporidium spp. are important pathogens causing diarrhea in humans and animals. However, few studies have been conducted on the infection of E. bieneusi and Cryptosporidium spp. in peafowl up to now. The purpose of the present study was to determine the prevalence and the involved genotypes of Cryptosporidium spp. and E. bieneusi in peafowl in Beijing and Jiangxi Province, China. In total, 258 peafowl fecal samples were collected. Overall, both Cryptosporidium spp. and E. bieneusi had the same prevalence, i.e. 6.59% (17/258). Higher infection rates of E. bieneusi and Cryptosporidium spp. were found in the adolescent peafowl. The prevalence of E. bieneusi in Beijing and Jiangxi Province was 5.23% and 8.57% respectively. For Cryptosporidium spp., the prevalence was 4.58% and 9.52% in Beijing and Jiangxi Province, respectively. Three zoonotic genotypes of E. bieneusi were confirmed, including two known genotypes, genotype Peru 6 and D, and one novel genotype, JXP1. Two avian specific species/genotypes of Cryptosporidium , Avian genotype Ⅲ and Goose genotype Ⅰ, were identified. To our knowledge, this is the first report of E. bieneusi and Cryptosporidium spp. occurrence in peafowl in China. The findings suggest that peafowl could be reservoirs of E. bieneusi and Cryptosporidium spp. which could be potentially transmitted to humans and other animals, and the present survey have implications for controlling E. bieneusi and Cryptosporidium spp. infection in peafowl.

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Section: Discussioncontrasting

confidence: 99%

First report of Enterocytozoon bieneusi and Cryptosporidium spp. in peafowl (Pavo cristatus) in China

Feng¹,

Han²,

Luo³

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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“…The novel genotypes SCT01 (0.3%, 1/266) and SCT02 (0.3%, 1/266) were only found in single specimens, both of which came from Yaan, and are the first newly-detected E. bieneusi genotypes from Tibetan pigs. Of the four genotypes identified in this study, EbpC was the most prevalent (69.9%, 58/83), and has been found in a number of animals, including cattle, dogs, cats, birds, non-human primates, bears, squirrels, sheep, foxes, deer, and humans [4, 8, 29, 36, 38, 39, 47, 51, 55]. EbpC is the prevalent E. bieneusi genotype associated with pig infection in China, reflecting E. bieneusi ’s dominance as a porcine parasite.…”

Section: Resultsmentioning

confidence: 88%

First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

et al. 2019

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Enterocytozoon bieneusi is a common intestinal pathogen in a variety of animals. While E. bieneusi genotypes have become better-known, there are few reports on its prevalence in the Tibetan pig. This study investigated the prevalence, genetic diversity, and zoonotic potential of E. bieneusi in the Tibetan pig in southwestern China. Tibetan pig feces (266 samples) were collected from three sites in the southwest of China. Feces were subjected to PCR amplification of the internal transcribed spacer (ITS) region. Enterocytozoon bieneusi was detected in 83 (31.2%) of Tibetan pigs from the three different sites, with 25.4% in Kangding, 56% in Yaan, and 26.7% in Qionglai. Prevalence varies according to age group, from 24.4% (age 0–1 years) to 44.4% (age 1–2 years). Four genotypes of E. bieneusi were identified: two known genotypes EbpC (n = 58), Henan-IV (n = 24) and two novel genotypes, SCT01 and SCT02 (one of each). We compare our results with a compilation of published results on the host range and geographical distribution of E. bieneusi genotypes in China. Phylogenetic analysis showed these four genotypes clustered to group 1 with zoonotic potential. Multilocus sequence typing (MLST) analysis of three microsatellites (MS1, MS3, MS7) and one minisatellite (MS4) was successful in 47, 48, 23 and 47 positive specimens and identified 10, 10, 5 and 5 genotypes at four loci, respectively. This study indicates the potential danger of E. bieneusi to Tibetan pigs in southwestern China, and offers basic advice for preventing and controlling infections.

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“…; Tavalla et al. 2018b). The genotype D was reported as the most frequent genotype worldwide, as well (Li et al.…”

Section: Discussionmentioning

confidence: 97%

The First Report and Molecular Analysis of Enterocytozoon bieneusi from Raccoon (Procyon lotor) in North of Iran

Javanmard

Nemati

Sharifdini

et al. 2020

J Eukaryotic Microbiology

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Microsporidia are known opportunistic microorganisms and usually transmitted via the fecal-oral route. However, there is no information about humaninfecting microsporidia in wildlife in Iran. This study aimed to investigate and analyze human-infecting microsporidia isolated from raccoons in north of Iran. Totally, 30 fecal samples were collected; then, DNA extraction was performed and specific fragments of the SSU rRNA gene of Enterocytozoon bieneusi and Encephalitozoon species were amplified. After amplification and sequencing the ITS, the results were compared to the GenBank database. Phylogenetic trees and network analysis were employed to explore probable relationships. E. bieneusi was the only detected microsporidia among samples. Genotyping showed the genotypes D, E, and RA in 15/18 (83.33%), 1/18 (5.55%), and 2/18 (11.11%) of samples, respectively. Novel genotypes RA1 and RA2 grouped together and apart from other genotypes. E. bieneusi genotypes D and E clustered with the genotypes previously reported from animals, humans, and environmental samples. Network analysis revealed six distinct sequence types among raccoon's isolates. This study demonstrated that E. bieneusi genotype D was the most prevalent microsporidia among raccoons. It seems that wildlife may play a role in dispersion of microsporidia spores.

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Molecular diagnosis of potentially human pathogenic Enterocytozoon bieneusi and Encephalitozoon species in exotic birds in Southwestern Iran

Cited by 27 publications

References 30 publications

First report of Enterocytozoon bieneusi and Cryptosporidium spp. in peafowl (Pavo cristatus) in China

First report of Enterocytozoon bieneusi and Cryptosporidium spp. in peafowl (Pavo cristatus) in China

First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

The First Report and Molecular Analysis of Enterocytozoon bieneusi from Raccoon (Procyon lotor) in North of Iran

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