Molecular Diagnostic for Boll Weevil (Coleoptera: Curculionidae) Based on Amplification of Three Species-Specific Microsatellites

Kim, Kyung Seok; Szendrei, Zsofia; Rodriguez‐Saona, Cesar; Mulder, P. G.; Sappington, Thomas W.

doi:10.1603/029.102.0237

Cited by 5 publications

(1 citation statement)

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“…Furthermore, the availability of diagnostic markers to resolve forms is important in helping management officials determine whether the form of boll weevil captured is a threat to cultivated cotton (Roehrdanz et al 2001). Molecular markers have been developed to discriminate boll weevils from other weevil species, such as the pepper weevil (Anthonomus eugenii), and are completely diagnostic, simple and economical to use (Kim et al 2009). However, marker development for use as a diagnostic tool to distinguish the three boll weevil forms has been problematic, in part due to difficulties in obtaining adequate geographic sampling of populations within the TW (Roehrdanz 2001) as well as Mexican (Scataglini et al 2006) ranges.…”

Section: Introductionmentioning

confidence: 99%

Variation in plant defense traits and population genetics within a Sonoran Desert cotton endemic, Gossypium davidsonii and boll weevil, Anthonomus grandis

Kuester

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CHAPTER1. General Introduction CHAPTER 2. Abiotic and biotic stress affects on defense trait phenotypes in a wild cotton species, Gossypium davidsonii CHAPTER 3. Response of herbivory to defense traits, geographic and genetic structure within a Sonoran Desert wild cotton species, Gossypium davidsonii CHAPTER 4. Comparison of quantitative defense traits and genetics in determining evolution of defense syndromes in a wild Sonoran Desert cotton endemic, Gossypium davidsonii CHAPTER 5. Covariation in the geographic genetic structures of a wild cotton, Gossypium davidsonii, and associated boll weevil, Anthonomus grandis CHAPTER 6. Population structure and genetic diversity of the boll weevil, Anthonomus grandis, in North America CHAPTER 7. General Conclusions APPENDIX. DNA Sequencing Research Rationale: Recognizing the mechanisms and patterns of evolution of natural plant defenses not only allows us to understand how to reasonably control host associated pest populations, but also to direct better-suited breeding and selection programs for traits associated with tolerance or resistance to otherwise uncontrollable pests (Gould 1988; Cortesero et al. 1999; Rausher 2001; Thrall et al. 2011). Though this thesis work focuses on Gossypium davidsonii, similar defense traits and responses to herbivory can be seen in many of the New World cotton species (Rudgers et al. 2004). Understanding how plants respond to herbivory in natural systems should provide us with insights into variation in traits that may provide resistance within agronomic settings. Additionally, population genetic studies of associated pest insects have identified approaches that best control associated insect herbivore populations (Porreta et al., 2007). Study System Gossypium davidsonii is a wild diploid cotton species that is endemic to the Sonoran Desert of northwestern Mexico, found in the subsection Integrifolia within the genus Gossypium (Family Malvaceae). It is commonly characterized as a branched shrub between 1-3 meters in height, with lobed to entire cordate leaves. The species is found from sea level to 400 m elevation with primary range within the lower Cape region of the Baja Peninsula. Habitat ranges from open to thorn-scrub vegetation in disturbed or rocky areas. Like most cotton species, G. davidsonii exhibits several characteristic defense traits. Though variation in nectary presence has been previously noted (Phillips and Clements, 1967), we detect no biological reason for selection on nectaries, as no myrmechophyllic 12 CHAPTER 2. Abiotic and biotic stress affects on defense trait phenotypes in a wild cotton species, Gossypium davidsonii

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Section: Introductionmentioning

confidence: 99%

Variation in plant defense traits and population genetics within a Sonoran Desert cotton endemic, Gossypium davidsonii and boll weevil, Anthonomus grandis

Kuester

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Spatial Distribution of Adult Anthonomus grandis Boheman (Coleoptera: Curculionidae) and Damage to Cotton Flower Buds Due to Feeding and Oviposition

et al. 2016

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The cotton boll weevil Anthonomus grandis Boheman (Coleoptera: Curculionidae) is the main pest in cotton crop around the world, directly affecting cotton production. In order to establish a sequential sampling plan, it is crucial to understand the spatial distribution of the pest population and the damage it causes to the crop through the different developmental stages of cotton plants. Therefore, this study aimed to investigate the spatial distribution of adults in the cultivation area and their oviposition and feeding behavior throughout the development of the cotton plants. The experiment was conducted in Maracaju, Mato Grosso do Sul, Brazil, in the 2012/2013 and 2013/2014 growing seasons, in an area of 10,000 m, planted with the cotton cultivar FM 993. The experimental area was divided into 100 plots of 100 m (10 × 10 m) each, and five plants per plot were sampled weekly throughout the crop cycle. The number of flower buds with feeding and oviposition punctures and of adult A. grandis was recorded throughout the crop cycle in five plants per plot. After determining the aggregation indices (variance/mean ratio, Morisita's index, exponent k of the negative binomial distribution, and Green's coefficient) and adjusting the frequencies observed in the field to the distribution of frequencies (Poisson, negative binomial, and positive binomial) using the chi-squared test, it was observed that flower buds with punctures derived from feeding, oviposition, and feeding + oviposition showed an aggregated distribution in the cultivation area until 85 days after emergence and a random distribution after this stage. The adults of A. grandis presented a random distribution in the cultivation area.

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Assessment of DNA Integrity From Trap-Captured Boll Weevil (Coleoptera: Curculionidae) for Use in a New PCR-Based Diagnostic Tool

Perkin

Oppert

Duke

et al. 2021

Journal of Economic Entomology

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The boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), is a major pest of commercial cotton (Gossypium hirsutum) in the southern United States and throughout Central and South America. Efforts are underway to develop a PCR-based diagnostic tool that can be used to rapidly and accurately differentiate boll weevils from other weevil species that are commonly captured in pheromone traps. However, the quantity and integrity of weevil DNA must be sufficient for a successful PCR assay. Currently, active eradication programs service traps weekly, but post-eradication programs service traps at 2- or 3-wk intervals. Consequently, captured weevils may be dead, dismembered, and exposed to environmental conditions for prolonged periods which may adversely affect the quantity and quality of weevil DNA. We documented DNA quantity and integrity in boll weevils and weevil body parts aged in traps over a 3-wk period under field conditions. The quantity of DNA extracted from whole weevils, heads, abdomens, and legs generally remained sufficient (> 1 ng/μl) for successful PCR amplification throughout the 21-d period. The integrity (fragment length) of extracted DNA declined over time but generally was sufficient (> 700 bp) for successful amplification. PCR amplification of three marker genes validated that the quality and integrity of DNA extracted from dead weevils and individual weevil body parts aged in traps up to 21 d remained at sufficient levels for the PCR-based assay. However, our data also suggested that rain events may accelerate degradation of weevil DNA.

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Molecular Diagnostic for Boll Weevil (Coleoptera: Curculionidae) Based on Amplification of Three Species-Specific Microsatellites

Cited by 5 publications

References 42 publications

Variation in plant defense traits and population genetics within a Sonoran Desert cotton endemic, Gossypium davidsonii and boll weevil, Anthonomus grandis

Variation in plant defense traits and population genetics within a Sonoran Desert cotton endemic, Gossypium davidsonii and boll weevil, Anthonomus grandis

Spatial Distribution of Adult Anthonomus grandis Boheman (Coleoptera: Curculionidae) and Damage to Cotton Flower Buds Due to Feeding and Oviposition

Assessment of DNA Integrity From Trap-Captured Boll Weevil (Coleoptera: Curculionidae) for Use in a New PCR-Based Diagnostic Tool

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