Molecular diagnostic methods for identifying Serrasalmid fish (Pacu, Pirapitinga, and Tambaqui) and their hybrids in the Brazilian aquaculture industry

Hashimoto, Diogo Teruo; Mendonça, Fernando Fernandes; Senhorini, José Augusto; Oliveira, Cláudio; Foresti, Fausto; Porto‐Foresti, Fábio

doi:10.1016/j.aquaculture.2011.08.018

Cited by 53 publications

(59 citation statements)

References 20 publications

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“…Problems related to wild populations produced for inappropriate crosses can also be damaging to aquaculture activity (Belle, Nash, 2009). In addition, the correct identification of the species contributes to genetic breeding and the integrity of natural stocks (Hashimoto et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

DNA barcoding confirms the occurrence of Rhamdia branneri and Rhamdia voulezi (Siluriformes: Heptapteridae) in the Iguaçu River Basin

et al. 2017

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DNA barcoding is a widely utilized molecular-based identification of species and taxonomic resolutions. Until recently, Rhamdia voulezi and Rhamdia branneri were considered species synonyms of Rhamdia quelen; however, morphological and cytogenetic analyses have suggested the validity of distinct species. Due to the absence of molecular taxonomy of R. voulezi and R. branneri, the objective of this study was to test its validity through traditional DNA barcoding and the GMYC (General Mixed Yule Coalescent) COI-based analyses in 19 specimens from the Iguaçu River Basin. In both methodologies, three MOTUs (Molecular Operational Taxonomic Units) were identified based on the estimated optimum threshold (OT = 0.77). The average inter-MOTU distance (NJ, K2P) between R. branneri and R. voulezi was 1.4%, and 0% intra-MOTU distance in both species. The two species identified as R. branneri and R. voulezi showed correspondence with taxonomic and morphological identifications. With regard to R. quelen, the average intra-MOTU distance was greater than OT (2.7%), indicating that this species can be formed by different MOTUs. We suggest that molecular and taxonomic studies should be employed concurrently in R. quelen, to prevent contamination of wild species by hybridizations. O DNA barcoding é uma ferramenta molecular precisa para a identificação de espécies e resoluções taxonômicas. Até recentemente, Rhamdia voulezi e Rhamdia branneri eram consideradas sinônimas de Rhamdia quelen, contudo caracteres morfológicos e citogenéticos têm apontado à validade de ambas. Devido à escassez de informações sobre a taxonomia molecular de R. voulezi e R. branneri, o objetivo do presente estudo foi testar a validade das mesmas através do método de DNA barcoding tradicional e GMYC (General Mixed Yule Coalescent), por meio da análise do gene COI em 19 espécimes do rio Iguaçu. Em ambos os métodos, três MOTUs (Unidades Taxonômicas Operacionais Moleculares) foram identificadas com base no ótimo threshold (OT = 0,77). A média inter-MOTU (NJ, K2P) entre R. branneri e R. voulezi foi 1,4%, com valores de 0% intra-MOTUs em ambas espécies. As duas espécies identificadas como R. voulezi e R. branneri apresentaram correspondência com a identificação taxonômica e morfológica dos respectivos vouchers. No que se refere a R. quelen, os resultados intra-MOTU foram superiores ao OT (2,7%), evidenciando a possibilidade de existirem diferentes MOTUs denominadas como R. quelen. Sugerimos que estudos moleculares e taxonômicos sejam empregados em R. quelen, para evitar a contaminação de espécies selvagens por hibridizações.Palavras-chave: Aquicultura, COI, Complexo de espécies, Jundiá, MOTU.

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Section: Discussionmentioning

confidence: 99%

DNA barcoding confirms the occurrence of Rhamdia branneri and Rhamdia voulezi (Siluriformes: Heptapteridae) in the Iguaçu River Basin

et al. 2017

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“…The occurrence of hybrids in natural environments is often closely associated with the escape and introduction of fish from cultivated stocks, where genetic manipulation is frequently not accompanied by effective monitoring and enforcement programs (Orsi and Agostinho, 1999;Fernandes et al, 2003;Hashimoto et al, 2011). Fish escapes into the natural environment is an alarming fact observed in the majority of the fish farms in Brazil (about 95% according to Castellani and Barrella, 2005), mainly due to flooding in the area where the fish are cultivated and even to the intentional introduction of fish species in rivers (Orsi and Agostinho, 1999;Castellani and Barrella, 2005).…”

Section: Incidence Of Hybridisationmentioning

confidence: 99%

Detection of hybrids and genetic introgression in wild stocks of two catfish species (Siluriformes: Pimelodidae): The impact of hatcheries in Brazil

et al. 2012

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“…As a result, data from the Brazilian Institute of Geography and Statistics (IBGE) shows that at least 70 different native fish species and their hybrids are farmed in Brazil, even though no legal benchmarks have ever been established for the husbandry and use of hybrid fish in farming systems (Hashimoto et al, 2011;Hashimoto et al, 2013;Hashimoto et al, 2014). Under such a scenario, setting quality standards at competitive prices for Brazilian aquaculture products is virtually impossible.…”

Section: Data Miningmentioning

confidence: 99%

Fish and fishery products trade in Brazil, 2005 to 2015: A review of available data and trends

Barone

Lorenz

Sonoda

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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Along the last ten years fish and fishery product trade in Brazil has been on a downward trajectory turning a profit of US$ 98.6 million in 2005 into a loss of US$ 1.25 billion by 2014. On the other hand, the country is a leading producer of grains and has the third largest animal feed industry in the world, which has added 5.5 million hectares of freshwater reservoirs and 3.5 million km 2 of an exclusive marine economic zone in the same period, a sizable potential for development of the aquaculture industry. This study aims at unveiling strategies for the reduction of the deficit in the Brazilian seafood trade balance, based on critical analysis of the quantitative and qualitative characteristics of imported fishery products. The fish and fishery product trade in Brazil, from 2005 to 2015, was studied considering import and export data mined from the Brazilian Ministry of Development, Industry and Commerce databases through the Aliceweb system, and clustered as follows: processing; product species; origin; conservation; and group.The main imported products were gutted fresh fish, highest price (salmon); salted dried fish, higher price (cod); frozen fish fillets, lowest price (fish and hake). The replacement of fish imports by domestic production is not enough to enable consumers to identify the equivalence between products (technical, qualitative or organoleptic). Developing strategies for the production of fish and fishery products at competitive prices and quantities that meet consumer demand is an immediate need, and the development of the aquaculture industry a rational strategy.

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Molecular diagnostic methods for identifying Serrasalmid fish (Pacu, Pirapitinga, and Tambaqui) and their hybrids in the Brazilian aquaculture industry

Cited by 53 publications

References 20 publications

DNA barcoding confirms the occurrence of Rhamdia branneri and Rhamdia voulezi (Siluriformes: Heptapteridae) in the Iguaçu River Basin

DNA barcoding confirms the occurrence of Rhamdia branneri and Rhamdia voulezi (Siluriformes: Heptapteridae) in the Iguaçu River Basin

Detection of hybrids and genetic introgression in wild stocks of two catfish species (Siluriformes: Pimelodidae): The impact of hatcheries in Brazil

Fish and fishery products trade in Brazil, 2005 to 2015: A review of available data and trends

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