Molecular diagnostics and ITS-based phylogenic analysis of Streptococcus suis serotype 2 in central Vietnam

Nguyen, Bach Hoang; Phan, Dieu Hong Nu; Nguyen, Hanh H.; Le, An N.; Alberti, Alberto

doi:10.3855/jidc.7027

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Recently, it was also recognized as an immunodominant antigen involved in the virulence of the Streptococcus species [6,8,17,18]. It is noteworthy that enolase is an antigenic protein recognised by patient sera with SS2 infection based on the immunoproteomics approach [19]. In this study, the enolase gene from SS2 was cloned and expressed to confirm its antigenic property.…”

Section: In Vitro Expression Of Enolase From Streptococcus Suis Serotmentioning

confidence: 94%

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In vitro expression of enolase from Streptococcus suis serotype 2 and its antigenicity

Nguyen¹,

Nguyen²,

Tore³

et al. 2018

VJSTE

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Abstract:Streptococcus suis serotype 2 (SS2) is one of the most important pathogens in the porcine industry and an important zoonotic agent. The absence of suitable vaccine or virulence markers makes SS2 infections more difficult to control. An immunoproteomics approach is used for identifying antigenic proteins in SS2 recognized enolase, which may represent strainspecific antigenic proteins and potential protective antigens. This study aims to clone, express enolase gene from SS2 and use western blotting to evaluate the antigenicity. Enolase gene from the SS2 strain was amplified with specific primers. The obtained PCR product was inserted into an expression vector, pGEX-4T1. The recombinant vector was then transformed into BL21 cells for protein expression. Subsequently, the immunological activity of the recombinant enolase was tested by western blotting with human sera in the convalescent phase. The glutathione S-transferase (GST)-tag fusion enolase was purified by glutathione sepharose affinity chromatography for further studies. The SS2 recombinant enolase was successfully expressed in E. coli. The western blotting analysis demonstrated that enolase has an antigenic property, which is recognized by patients naturally infected with SS2.

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Section: In Vitro Expression Of Enolase From Streptococcus Suis Serotmentioning

confidence: 94%

“…Bacterial strain and patient sera collection SS2 strains were isolated and identified in patients infected with Streptococcus suis serotype 2, as described in previous publication [19]. The patient sera were collected from the SS2 infected patients in convalescent phases who were hospitalized for treatment.…”

Section: Methodsmentioning

confidence: 99%

In vitro expression of enolase from Streptococcus suis serotype 2 and its antigenicity

Nguyen¹,

Nguyen²,

Tore³

et al. 2018

VJSTE

Self Cite

View full text Add to dashboard Cite

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Streptococcosis

Gottschalk¹,

Segura²

2019

Diseases of Swine

116

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Development of Multiplex Real-Time PCR Assay for Six Pathogens: Rapid Tool for Diagnosis of Bacterial Meningitis

Ngo

Nguyen

et al. 2019

JMP

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Introduction: Bacterial meningitis is an acute central nervous infection with high mortality or permanent neurological sequelae if remained undiagnosed. However, traditional diagnostic methods for bacterial meningitis pose challenge in prompt and precise identification of causative agents. Aims: The present study will therefore aim to set up in-house PCR assays for diagnosis of six pathogens causing the disease including H. influenzae type b, S. pneumoniae, N. meningitidis, S. suis serotype 2, E. coli and S. aureus. Methods: inhouse PCR assays for detecting six above-mentioned bacteria were optimized after specific pairs of primers and probes collected from the reliable literature resources and then were performed for cerebrospinal fluid (CSF) samples from patients with suspected meningitis in Hue Hospitals. Results: The set of four PCR assays was developed including a multiplex real-time PCR for S. suis serotype 2, H. influenzae type b and N. meningitides; three monoplex real-time PCRs for E. coli, S. aureus and S. pneumoniae. Application of the in-house PCRs for 116 CSF samples, the results indicated that 48 (39.7%) cases were positive with S. suis serotype 2; one case was positive with H. influenzae type b; 4 cases were positive with E. coli; pneumococcal meningitis were 19 (16.4%) cases, meningitis with S. aureus and N. meningitidis were not observed in any CSF samples in this study. Conclusion: our in-house real-time PCR assays are rapid, sensitive and specific tools for routine diagnosis to detect six mentioned above meningitis etiological agents. Key words: Bacterial meningitis, etiological agents, multiplex real-time PCR

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Molecular diagnostics and ITS-based phylogenic analysis of Streptococcus suis serotype 2 in central Vietnam

Cited by 3 publications

References 26 publications

In vitro expression of enolase from Streptococcus suis serotype 2 and its antigenicity

In vitro expression of enolase from Streptococcus suis serotype 2 and its antigenicity

Streptococcosis

Development of Multiplex Real-Time PCR Assay for Six Pathogens: Rapid Tool for Diagnosis of Bacterial Meningitis

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