Molecular diagnostics of clinically important staphylococci

Štěpán, Jiří; Pantůček, Roman; Doškař, Jiřı́

doi:10.1007/bf03354664

Cited by 29 publications

(8 citation statements)

References 378 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since 16S rRNA analysis has limited discriminatory power for identifying some staphylococcal species, the phylogenetic position of SIG strains assigned by the 16S rRNA gene sequencing was assessed using the sequence data of the partial rpoB gene that was previously shown to be effective for the species differentiation of staphylococci [40,61]. The computed neighbor-joining phylogenetic tree based on the partial rpoB gene sequence showed that SIG isolates clustered into two major groups corresponding to S. delphini and S. pseudintermedius ( Figure 2).…”

Section: Rpob Gene Sequencingmentioning

confidence: 99%

Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini

et al. 2020

View full text Add to dashboard Cite

Members of the genus Staphylococcus are widespread in nature and occupy a variety of niches, however, staphylococcal colonization of animals in the Antarctic environment has not been adequately studied. Here, we describe the first isolation and characterization of two Staphylococcus intermedius group (SIG) members, Staphylococcus delphini and Staphylococcus pseudintermedius, in Antarctic wildlife. Staphylococcus delphini were found exclusively in Adélie penguins. The report of S. pseudintermedius from Weddell seals confirmed its occurrence in all families of the suborder Caniformia. Partial RNA polymerase beta-subunit (rpoB) gene sequencing, repetitive PCR fingerprinting with the (GTG)5 primer, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry gave consistent identification results and proved to be suitable for identifying SIG members. Comparative genomics of S. delphini isolates revealed variable genomic elements, including new prophages, a novel phage-inducible chromosomal island, and numerous putative virulence factors. Surface and extracellular protein distribution were compared between genomes and showed strain-specific profiles. The pathogenic potential of S. delphini was enhanced by a novel type of exfoliative toxin, trypsin-like serine protease cluster, and enterotoxin C. Detailed analysis of phenotypic characteristics performed on six Antarctic isolates of S. delphini and eight reference strains from different animal sources enabled us to emend the species description of S. delphini.

show abstract

Section: Rpob Gene Sequencingmentioning

confidence: 99%

Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Second malevolence GPB causing pneumonia are Staphylococcus spp, especially S. aureus mainly if it harbours methicillin or other antibiotic resistance and/or virulent factors as Panton-Valentine Leukocydin (PVL), cell interfering clumping factors, etc. [27,32,33]. Other bacteria also involved in causing infectious in the LTR can be Chlamydia pneumoniae, and Clostridium difficile.…”

Section: Bacterial Infectionsmentioning

confidence: 99%

“…The protocols developed allowed to elucidate the existing association between the presence of the Panton-Valentine leukocidin-encoding gene and a lower rate of survival among hospitalized pulmonary patients with staphylococcal infection [12]. Moreover, combination of different molecular typing methods with classical microbiology ones has permitted us to build up solid MRSA epidemiology studies at both global level and local stage [33,[39][40][41][42][43][44]. These studies constitute a corner stone for the establishment of prevention and treatment measures and can be divided according to the utilization of DNA amplification [45,46].…”

Section: Staphylococcus Aureus Pulmonary Infections Molecular Diagnomentioning

confidence: 99%

“…Several works have tried to determine if MLVA provides enough information to be performed routinely instead of PFGE or MLST, which are more laborious, in the clinical setting. In our laboratory, MLVA was performed as previously described [51], but slightly modified to obtain optimal results and to accelerate the process [33,52,53] concluding that the utilization of MLVA allows to distinguish among different MRSA reservoirs and other circulating MRSA strains. The proven simplicity, low cost, and speed of MLVA enable the performance of routine checkups in patients, mainly via admission screening on surgical wards and in intensive care units, hampering the spread of these strains and therefore reducing the morbidity, mortality, and costs [28,44,54,55].…”

Section: Multiple-locus Variable Number Tandemmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Microbial Diagnostic in Lung Transplant Recipients

Méndez-Álvarez¹,

Pulido-Reyes²,

Donate‐Correa³

et al. 2012

JCT

View full text Add to dashboard Cite

The appearing of microbial lineages carrying multiple dangerous loci are results from the extensive use of antibiotics and has brought a huge increase in the infections-derived morbidity and mortality, which is critical in the hospital setting. Etiologic agents of these non easily tractable infections include bacteria, such as the genus Staphylococcus, but also fungi and virus. Alterations in the immune system allow these organisms to invade and affect the functionality of any tissue, organ or system of the human being. Pulmonary infection occurs as result of deficient lung systemic defence mechanisms which could be altered by medical treatments or by environmental factors. The infective agents commonly gain access to the lung by air, but also by blood or lymphatic system. Staphylococcus aureus strains that share antibiotic resistance and virulence factors represent the aetiological agent responsible of many cases of bacterial pneumonia, thoracic surgery postoperative infections, and diverse tissue infections, resulting in significant disease and morbidity in recipient patients after lung transplantation. The control and treatment of Staphylococcus infections, especially methicillin resistant strains, need for developing reliable and rapid methods of detection and characterization of these microorganisms. Nowadays, new insights into the diagnostic and epidemiology of MRSA and other pathogenic staphylococci have been developed employing molecular methods. This has meant an important advance in the diagnostic and treatment plans of such infective bacteria

show abstract

“…A variety of molecular methods have been extensively used for typing S. aureus isolates, of which staphylococcal protein-A (spa) typing is one among the most common. spa typing is suitable for epidemiological studies and produces reproducible, unambiguous, and easily interpreted results [3]. Currently, the growing spa typing database developed by Harmsen et al [4] is the largest database for typing S. aureus, surpassing that of multilocus sequence typing.…”

Section: Introductionmentioning

confidence: 99%

Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples

Santos

Souza

Oliveira

et al. 2020

Animals

View full text Add to dashboard Cite

In the present study, we aimed to determine the antimicrobial resistance and molecular typing of Staphylococcus aureus recovered from transient and persistent intramammary infections and nares/muzzles in dairy cows. We investigated the antimicrobial resistance of 189 S. aureus strains using a broad antimicrobial susceptibility profile. Furthermore, 107 S. aureus isolates were strain-typed using staphylococcal protein-A (spa) typing. A large proportion of strains exhibited multidrug resistance to antimicrobials, including resistance to critically important antimicrobials, although no methicillin-resistant S. aureus strains were found. Our study did not strengthen the idea that extramammary niches (i.e., nares/muzzles) are an important source of S. aureus for bovine mastitis. A discrepancy in the antimicrobial resistance between S. aureus strains isolated from nares/muzzles and milk samples was observed. Furthermore, S. aureus isolates from transient and persistent intramammary infections (IMIs) did not differ by spa typing, suggesting that the persistence of bovine IMIs was determined by cow factors. Thus, the high level of multidrug-resistant S. aureus found in the two herds, considered together with the predominance of a well udder-adapted S. aureus strain, may contribute to our knowledge of the history of the high prevalence of mastitis caused by S. aureus, which is of great concern for animal and public health.

show abstract

Molecular diagnostics of clinically important staphylococci

Cited by 29 publications

References 378 publications

Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini

Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini

Molecular Microbial Diagnostic in Lung Transplant Recipients

Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples

Contact Info

Product

Resources

About