Molecular differentiation of five Sarcocystis species in sika deer (Cervus nippon centralis) in Japan based on mitochondrial cytochrome c oxidase subunit I gene (cox1) sequences

Abe, Niichiro; Matsuo, Kayoko; Moribe, Junji; Takashima, Yasuhiro; Baba, Takashi; Gjerde, Bjørn

doi:10.1007/s00436-019-06319-5

Cited by 12 publications

(20 citation statements)

References 13 publications

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“…We have here relied on molecular methods for the identification of Sarcocystis species affecting red deer and putative DH foxes and dogs. Molecular studies are necessary because different Sarcocystis species affecting cervids have a highly similar cyst morphology by light and electron microscopy ( Dahlgren and Gjerde, 2010a ; Gjerde et al, 2017b ; Abe et al, 2019a ), and oocysts and sporocysts in the DH cannot be discriminated by microscopic techniques ( Dubey et al, 2016 ). Several studies based the identification of Sarcocystis at the species level on the 18S ribosomal RNA gene sequence as it was performed in this study, but also the 28S rRNA gene, the ITS1 region, the mitochondrial cytochrome c oxidase subunit I gene ( cox 1) or different combinations of these targets have been used for this purpose, enhancing the diagnostic possibilities ( Gjerde 2013 , 2014b ; Gjerde et al, 2017b ; Moré et al, 2016 ; Dahlgren and Gjerde, 2010a ; Cerqueira-Cézar et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…Cunters), sequences with 100 and 99.9% identity with GenBank® sequences of S. pilosa from sika deer ( Cervus nippon ) LC466183 ( Irie et al, 2019 ) were detected. Sarcocystis pilosa has been so far described infecting sika deer in Lithuania ( Prakas et al, 2016 ) and Japan ( Abe et al 2019a , 2019b ; Irie et al, 2019 ), but there are no previous records of infection in red deer. Our findings are highly suggestive that red deer could also act as IH of this parasite.…”

Section: Discussionmentioning

confidence: 99%

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Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Basso

Rojas

Buob

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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Red deer ( Cervus elaphus ) carcasses showing grey-greenish discolouration have been increasingly observed in the canton of Grisons, Switzerland. We investigated whether Sarcocystis infections were associated with this pathology, and whether wild and domestic canids were involved in their transmission. Meat from affected red deer ( n = 26), faeces and intestines from red foxes ( Vulpes vulpes ) ( n = 126), and faeces from hunting dogs ( n = 12) from the region, were analysed. Eosinophilic myositis and/or fasciitis were diagnosed in 69% of the deer, and sarcocysts were observed in 89% of the animals. Molecular typing targeting a ~700bp variable region of the 18S rRNA gene revealed Sarcocystis hjorti in 73%, S. venatoria/S. iberica in 54%, S. linearis/S. taeniata in 12%, S. pilosa in 8% and S. ovalis in 4% of the deer samples. No inflammatory changes were observed in red deer carcasses with normal appearance ( n = 8); however, sarcocysts were observed in one sample, and S. hjorti , S. venatoria/S. iberica or S. silva DNA was detected in five samples. Sarcocystis oocysts/sporocysts were observed in 11/106 faecal and 6/20 intestinal fox samples, and in 2/12 canine samples. Sarcocystis tenella ( n = 8), S. hjorti ( n = 2), S. gracilis ( n = 2), and S. miescheriana ( n = 1) were identified in foxes, and S. gracilis ( n = 2), S. capreolicanis ( n = 1) and S. linearis/S. taeniata ( n = 1) in dogs. This study provides first molecular evidence of S. pilosa and S. silva infection in red deer and S. linearis/S. taeniata in dogs and represents the first record of S. ovalis transmitted by corvids in Central Europe. Although Sarcocystis species infecting red deer are not regarded as zoonotic, the affected carcasses can be declared as unfit for human consumption due to the extensive pathological changes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Basso

Rojas

Buob

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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“…Similarly, Irie et al (2019), identified three sarcocyst types in histological sections of the diaphragm of 65 Hokkaido sika deer ( Cervus nippon yesoensis ) from Hokkaido, Japan, and characterized six DNA isolates of each morphological type at 18S rDNA and cox1 following laser microdissection of sections of formalin fixed sarcocysts. They identified three sequence types at both markers used ( Sarcocystis truncata -like, S. tarandi -like, S. pilosa ), which corresponded to Types 1, 2 and 4 of our recent study (Abe et al, 2019), but they did not name the species with type 1 sarcocysts and sequences.…”

Section: Introductionmentioning

confidence: 83%

“…Genomic DNA from all 220 sarcocysts was initially tested separately using the previously described multiplex PCR assay (Abe et al, 2019) with a slight modification of the reaction conditions. This assay was able to detect and discriminate between five Sarcocystis spp.…”

Section: Methodsmentioning

confidence: 99%

“…As regards the use of the cox1 marker for identification of Sarcocystis spp. in sika deer in Japan, we recently obtained cox1 sequences from 21 sarcocysts from nine sika deer in Gifu prefecture, and identified five Sarcocystis species, of which three species (Types 1, 3 and 5) seemed to be new, that is, not previously well characterized and named (Abe et al, 2019). However, the sarcocysts used in that study, which were obtained from the study by Matsuo et al (2016), were only cursorily examined under a stereo microscope in connection with their isolation, and hence no detailed morphological data were recorded for each sarcocyst.…”

Section: Introductionmentioning

confidence: 99%

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Morphological and molecular characteristics of seven Sarcocystis species from sika deer (Cervus nippon centralis) in Japan, including three new species

Abe

Matsuo

Moribe

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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Samples of diaphragm were collected from 53 sika deer from Gifu Prefecture, Japan; 220 sarcocysts were isolated, examined in wet mounts and classified according to their cyst wall protrusions. The sarcocysts were then examined molecularly in order to assign them to different species. All but 11 of the 220 sarcocysts were initially identified by means of a multiplex PCR assay targeting cox1 of five species, whereas the remaining 11 sarcocysts were identified by standard PCR and sequencing. DNA from selected sarcocysts was used for PCR amplification and sequencing of cox1 (59 sequences) and 18S rDNA (23 sequences). The 220 sarcocysts comprised seven major cox1 sequence types or species. Types 4 and 7 were assigned to the known species Sarcocystis pilosa and Sarcocystis ovalis, whereas types 1, 3 and 5 were considered to represent three new species, for which the names Sarcocystis japonica, Sarcocystis matsuoae and Sarcocystis gjerdei have been proposed. Types 2 and 6 were most similar to Sarcocystis tarandi and Sarcocystis taeniata, respectively, but could not be unequivocally assigned to these species. Sarcocysts belonging to S. japonica were macroscopic with fairly thick finger-like protrusions, whereas most sarcocysts of the six other species were microscopic. Sarcocysts of S. cf. tarandi and S. matsuoae were spindle-shaped and possessed thin finger-like cyst-wall protrusions. Sarcocysts of S. pilosa and S. gjerdei had similar hair-like protrusions, whereas those of S. cf. taeniata had a smooth surface. Sarcocysts of S. japonica, S. pilosa, S. cf. tarandi, S. gjerdei, S. matsuoae, S. cf. taeniata and S. ovalis were found in 50 (94.3%), 29 (54.7%), 22 (41.5%), 10 (18.9%), 8 (15.1%), 6 (11.3%) and 1 (1.9%) of the 53 sika deer examined, respectively. An improved multiplex PCR assay targeting cox1 was developed, through which the seven Sarcocystis spp. found in the present study could be identified.

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Presence of Sarcocystis sybillensis in Japanese sika deer (Cervus nippon) captured in its native territory and its phylogenetic relationship with Sarcocystis nipponi

et al. 2020

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Molecular differentiation of five Sarcocystis species in sika deer (Cervus nippon centralis) in Japan based on mitochondrial cytochrome c oxidase subunit I gene (cox1) sequences

Cited by 12 publications

References 13 publications

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Sarcocystis infection in red deer (Cervus elaphus) with eosinophilic myositis/fasciitis in Switzerland and involvement of red foxes (Vulpes vulpes) and hunting dogs in the transmission

Morphological and molecular characteristics of seven Sarcocystis species from sika deer (Cervus nippon centralis) in Japan, including three new species

Presence of Sarcocystis sybillensis in Japanese sika deer (Cervus nippon) captured in its native territory and its phylogenetic relationship with Sarcocystis nipponi

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