2021
DOI: 10.1002/syst.202100025
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Molecular Display on Protein Nanocompartments: Design Strategies and Systems Applications

Abstract: The design of biomimetic systems in the laboratory is a long‐sought goal for systems chemists and synthetic biologists alike. Fundamental to this design is the generation of self‐assembled structures capable of mimicking compartmentalisation, which includes the encapsulation of molecular cargo as well as the display of molecules on the exterior. Protein nanocompartments are fast becoming popular scaffolds for these systems due to their robust self‐assembly, ability to encapsulate non‐native cargo, and amenabil… Show more

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Cited by 12 publications
(8 citation statements)
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References 100 publications
(275 reference statements)
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“…There are several methods used to display molecules on the surface of encapsulin shells, including chemical conjugation, genetic fusion, and post-translational protein-mediated covalent attachment, as reviewed by Szyszka et al 51 . Chemical conjugation is a popular method for the attachment of dyes, fluorophores, or other small molecules 32,36,46,52 , while genetic fusion is a popular method for the attachment of small peptides or heterologous proteins 32,36,45,53 .…”
Section: Molecular Display On the Encapsulin Surfacementioning
confidence: 99%
“…There are several methods used to display molecules on the surface of encapsulin shells, including chemical conjugation, genetic fusion, and post-translational protein-mediated covalent attachment, as reviewed by Szyszka et al 51 . Chemical conjugation is a popular method for the attachment of dyes, fluorophores, or other small molecules 32,36,46,52 , while genetic fusion is a popular method for the attachment of small peptides or heterologous proteins 32,36,45,53 .…”
Section: Molecular Display On the Encapsulin Surfacementioning
confidence: 99%
“…The self-assembly of proteins can be harnessed to produce intricate nanostructures that can be functionalized with additional protein domains to create nanodevices for a variety of applications [ 1 ]. Functionalized protein nanostructures are generally composed of many different protein subunits, with some subunits assembling as an architectural scaffold [ 2 4 ], while other subunits serve as functional components to produce chemical activities. The functional protein subunits are attached to the scaffold, whereby their proximity and alignment results in emergent properties of the whole assembly.…”
Section: Introductionmentioning
confidence: 99%
“…In addition to their native functions, protein compartments can be expressed recombinantly and packaged with non-native biomolecular or synthetic cargo for downstream in vivo and in vitro engineering applications. From an engineering perspective, an appealing feature that distinguishes protein compartments from other biomolecular compartments, such as lipid vesicles or membraneless condensates, is their ability to robustly self-assemble from simple subunits to form homogeneous three-dimensional architectures that are defined at the molecular level. The past decade has seen modified and de novo protein compartments used as modular systems for catalysis and drug delivery in the fields of synthetic biology and nanotechnology. …”
Section: Introductionmentioning
confidence: 99%