Molecular diversity among five different endogenous primate retroviruses

Bryant, Martin L.; Sherr, Charles J.; Sen, Arup; Todaro, George J.

doi:10.1128/jvi.28.1.300-313.1978

Cited by 26 publications

(3 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Baboon endogenous virus (BaEV) is a type C retrovirus present in multiple proviral copies in the DNA of baboons (10). With one exception (RD-114), no homology between BaEV and any of the other known mammalian type C or type D retroviruses has been found by liquid hybridization experiments under conditions of moderate stringency (12,33,37,42,43). In contrast, recent DNA sequence analyses of BaEV and AKR murine leukemia virus (AKR-MuLV) recombinant DNA clones revealed that their gag p30 genes contain a sequence of at least 35 identical nucleotides (14a; R. Stephens, R. Mural, and M. Cohen, unpublished data).…”

mentioning

confidence: 98%

DNA Sequence Relationship of the Baboon Endogenous Virus Genome to the Genomes of Other Type C and Type D Retroviruses

Cohen¹,

Rice²,

Stephens³

et al. 1982

J Virol

View full text Add to dashboard Cite

Baboon endogenous virus (BaEV) is a type C retrovirus present in multiple proviral copies in the DNA of baboons. Although interspecies antigenic determinants present on reverse transcriptase and gag proteins are shared among all mammalian type C viruses, no nucleic acid homology between BaEV and other type C viruses (except RD-114) has been found in conventional liquid hybridization experiments. In this study, we used restriction fragments of cloned BaEV DNA immobilized on nitrocellulose to test for relatedness with [ 32 P]cDNA's of various type C and type D viruses. We detected the following distant relationships previously found only through immunological and protein sequencing techniques: (i) eight type C viral cDNA's (the endogenous virus of rhesus monkeys, feline leukemia virus, simian sarcoma virus, gibbon ape leukemia virus, Rauscher murine leukemia virus, BALB-2, NZB, and RD-114) and two type D viral cDNA's (Mason-Pfizer monkey virus and squirrel monkey retrovirus) were able to hybridize with cloned BaEV DNA; (ii) the eight type C probes hybridized to restriction fragments spanning most of the BaEV genome, but only RD-114 hybridized to fragments within the 1.9 kilobases at the 3′ end of the genome; (iii) the two type D probes hybridized primarily to fragments within the 1.9 kilobases at the 3′ terminus and weakly or not at all elsewhere; and (iv) [ 32 P]cDNA's of several other oncornaviruses (mouse mammary tumor virus, equine infectious anemia virus, bovine leukemia virus, and reticuloendotheliosis virus) exhibited no homology with BaEV DNA. DNA sequence analysis has allowed us to orient the BaEV restriction map with the genetic map at both ends of the genome. Homologies between retroviral cDNA's and BaEV clone restriction fragments could thus be related to specific BaEV genes. Whereas type C cDNA's hybridized to fragments from gag, pol , and the pol-env junction, squirrel monkey retrovirus cDNA hybridized only to a fragment coding for the p15E portion of env. Mason-Pfizer monkey virus cDNA also hybridized within the p15E region, but exhibited homology to the 3′ half of gp70 as well. These results are discussed relative to previously reported antigenic relatedness of retroviral proteins. The data suggest that BaEV represents an important link in oncornavirus evolution.

show abstract

mentioning

confidence: 98%

DNA Sequence Relationship of the Baboon Endogenous Virus Genome to the Genomes of Other Type C and Type D Retroviruses

Cohen¹,

Rice²,

Stephens³

et al. 1982

J Virol

View full text Add to dashboard Cite

show abstract

“…2. On a few occasions, however, overexposed autoradiograms from experiments using high-specific-activity generalized MuLV probes revealed hybridization to BamHI fragment 7 (1.8 kb) of the monkey clone in addition to significantly stronger reactions with BamHI fragments 6, 9, and 10 ( Fig. 7A).…”

Section: Resultsmentioning

confidence: 99%

“…The location of the MuLV-related segment within the monkey clone would predict that sequences analogous to the 3'-terminal 1.9-kb BamHI fragment of AKR MuLV DNA and its associated LTR segment might be present in BamHI fragment 7 (Fig. 5) of X-AGM-1 DNA.…”

Section: Resultsmentioning

confidence: 99%

Detection and cloning of murine leukemia virus-related sequences from African green monkey liver DNA

Martin¹,

Bryan²,

McCutchan³

et al. 1981

J Virol

View full text Add to dashboard Cite

By using low-stringency nucleic acid hybridization conditions and specific subgenomic segments of the AKR ecotropic provirus as probes, murine leukemia virus (MuLV)-related sequences were detected in African green monkey (AGM) liver DNA. The MuLV-reactive segments present in restricted AGM DNA ranged from 1.9 kilobases (kb) to greater than 10 kb in size. On the basis of this finding, a 17-kb segment was cloned from a partial EcoRI AGM library in lambda Charon 4A which shared nearly 5 kb of homology with AKR ecotropic MuLV DNA. The MuLV-related sequences detected in restricted preparations of AGM DNA or present in the cloned monkey DNA reacted with probes mapping 2.0 to 7.0 kb from the 5' terminus of the AKR ecotropic provirus. The AGM clone also contained repeated sequences that flanked the MuLV-related segment. Labeled, subgenomic, MuLV-reactive segments of the monkey clone hybridized to multiple restriction fragments of AGM liver DNA, indicating the presence of several copies of the MuLV-related sequences.

show abstract

Primate Retroviruses and AIDS

Gardner

1985

RNA Tumor Viruses, Oncogenes, Human Cancer and AIDS: On the Frontiers of Understanding

View full text Add to dashboard Cite

Molecular diversity among five different endogenous primate retroviruses

Abstract: A204 (18), the human lung carcinoma cell line, A549 (25), rhesus monkey lung cells, DBS-FRhL-1 (47), the bat lung cell line TblLu (CCL 88, ATCC), fetal cat 300

Cited by 26 publications

References 46 publications

DNA Sequence Relationship of the Baboon Endogenous Virus Genome to the Genomes of Other Type C and Type D Retroviruses

DNA Sequence Relationship of the Baboon Endogenous Virus Genome to the Genomes of Other Type C and Type D Retroviruses

Detection and cloning of murine leukemia virus-related sequences from African green monkey liver DNA

Primate Retroviruses and AIDS

Contact Info

Product

Resources

About