Molecular diversity and detection of<i>Waxy</i>genes in the Iranian wheat populations by multiplex PCR

Maryami, Zahra; Fazeli, Arash

doi:10.1080/13102818.2015.1045940

Cited by 3 publications

(1 citation statement)

References 34 publications

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“…To increase the genetic diversity of wheat, genes from primary, as well as secondary and tertiary, pools are used. The efforts in searching for naturally evolved waxy alleles are mainly concentrated on the primary and secondary gene pool, i.e., bread and durum wheats, einkorn, emmer and spelt collections, and wild Aegilops and Triticum germplasm [8,14,17,[23][24][25][26][27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Waxy Gene-Orthologs in Wheat × Thinopyrum Amphidiploids

et al. 2020

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Starch, as the main component of grain in cereals, serves as the major source of calories in staple food and as a raw material for industry. As the technological and digestive properties of starch depend on its content, the management of its components, amylose and amylopectin, is of great importance. The starch properties of wheat grain can be attuned using allelic variations of genes, including granule-bound starch synthase I (GBSS I), or Wx. The tertiary gene pool, including wheatgrass (Thinopyrum) species, provides a wide spectrum of genes-orthologs that can be used to increase the allelic diversity of wheat genes by wide hybridization. Octaploid partial wheat–wheatgrass hybrids (amphidiploids, WWGHs) combine the complete genome of bread wheat (BBAADD), and a mixed genome from the chromosomes of intermediate wheatgrass (Thinopyrum intermedium, genomic composition JrJrJvsJvsStSt) and tall wheatgrass (Th. ponticum, JJJJJJJsJsJsJs). Thus, WWGHs may carry Wx genes not only of wheat (Wx-B1, Wx-A1 and Wx-D1) but also of wheatgrass origin. We aimed to assess the level of amylose in starch and investigate the polymorphism of Wx genes in 12 accessions of WWGHs. Additionally, we characterized orthologous Wx genes in the genomes of wild wheat-related species involved in the development of the studied WWGHs, Th. intermedium and Th. ponticum, as well as in the putative donors of their subgenomes, bessarabian wheatgrass (Th. bessarabicum, JbJb) and bluebunch wheatgrass (Pseudoroegneria stipifolia, St1St1St2St2). Although no significant differences in amylose content were found between different WWGH accessions, SDS-PAGE demonstrated that at least two WWGHs have an additional band. We sequenced the Wx gene-orthologs in Th. bessarabicum, P. stipifolia, Th. intermedium and Th. ponticum, and developed a WXTH marker that can discriminate the Thinopyrum Wx gene in the wheat background, and localized it to the 7E chromosome in Th. elongatum. Using the WXTH marker we revealed the allelic polymorphism of the Thinopyrum Wx gene in the studied WWGHs. The applicability of Thinopyrum Wx genes in wheat breeding and their effect on starch quality are discussed.

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Section: Introductionmentioning

confidence: 99%

Waxy Gene-Orthologs in Wheat × Thinopyrum Amphidiploids

et al. 2020

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Comparative non-destructive classification of partial waxy wheats using near-infrared and Raman spectroscopy

Liu

Gao

et al. 2019

Crop Pasture Sci.

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Waxy proteins play a key role in amylose synthesis in wheat. Eight lines of common wheat (Triticum aestivum L.) carrying mutations in the three homoeologous waxy loci, Wx-A1, Wx-B1 and Wx-D1, have been classified by near-infrared (NIR) and Raman spectroscopy combined with chemometrics. Sample spectra from wheat seeds were collected by using a NIR spectrometer in the wave rage 1600–2400 nm, and then Raman spectrometer in the wave range 700–2000 cm–1. All samples were split randomly into a calibration sample set containing 284 seeds (~35 seeds per line) and a validation sample set containing the remaining 92 seeds. Classification of these samples was undertaken by discriminant analysis combined with principal component analysis (PCA) based on the raw spectra processed by appropriate pre-treatment methods. The classification results by discriminant analysis indicated that the percentage of correctly identified samples by NIR spectroscopy was 84.2% for the calibration set and 84.8% for the validation set, and by Raman spectroscopy 94.4% and 94.6%, respectively. The results demonstrated that Raman spectroscopy combined with chemometrics as a rapid method is superior to NIR spectroscopy in classifying eight partial waxy wheat lines with different waxy proteins.

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Phenotypic and genetic diversity of leaf rust resistance in wheat wild relatives

Sadeghabad

Dadkhodaie

Heidari

2020

Journal of Phytopathology

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Puccinia triticina (Pt), the causal agent of leaf rust evolves through forming new pathotypes that adversely affect the growth and yield of wheat cultivars. Therefore, continued production of resistant varieties through exploring novel sources of resistance in wild relatives which are abundantly found in Iran and the neighbouring regions is a major task in wheat breeding programs. The aim of the present study was to explore 60 wild wheat genotypes selected from the species Triticum monococcum, Aegilops tauschii, Ae. neglecta, Ae. cylindrica, Ae. triuncialis, Ae. umbellulata, Ae. speltoides, Ae. columnaris, Ae. crassa and Ae. ventricosa for resistance to leaf rust. The cultivar ‘Boolani’ and Thatcher near‐isogenic lines were used as controls. Two‐week‐old seedlings were inoculated using 10 Pt pathotypes, and the infection types were recorded. The genotypes were also analysed for polymorphism using six sequence‐tagged sites (STS) and sequence characterized amplified region (SCAR) markers. Forty‐eight genotypes produced high infection types (3+) for two pathotypes, but the remaining genotypes produced low infection types of ‘0; =’ to ‘1+CN’ to all pathotypes. The latter included three accessions of Ae. tauschii, two accessions of each Ae. umbellulata, Ae. columnaris and Triticum monococcum, and one accession from each Ae. triuncialis, Ae. ventricosa and Ae. neglecta. Analysis for STS and SCAR markers suggested several genotypes could carry the genes Lr9, Lr10, Lr19, Lr24, Lr26 and Lr37 or their potential orthologs in addition to unknown resistance genes. In conclusion, the identified resistant genotypes could be further characterized and used in wheat breeding programs for leaf rust resistance.

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Molecular diversity and detection ofWaxygenes in the Iranian wheat populations by multiplex PCR

Cited by 3 publications

References 34 publications

Waxy Gene-Orthologs in Wheat × Thinopyrum Amphidiploids

Waxy Gene-Orthologs in Wheat × Thinopyrum Amphidiploids

Comparative non-destructive classification of partial waxy wheats using near-infrared and Raman spectroscopy

Phenotypic and genetic diversity of leaf rust resistance in wheat wild relatives

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