Molecular Diversity in <i>Puccinia triticina</i> Isolates from Ethiopia and Germany

Mebrate, S. A.; Dehne, H. W.; Pillen, Klaus; Oerke, E. C.

doi:10.1111/j.1439-0434.2006.01177.x

Cited by 20 publications

(22 citation statements)

References 39 publications

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“…Eventually, host selection due to the narrow genetic make‐up reduces the genetic diversity in the pathogen population. In a similar manner, high genetic diversity within the P. tritici population from central Ethiopia has been reported compared to south and southeast Ethiopian regions (Mebrate et al. 2006).…”

Section: Discussionsupporting

confidence: 60%

Genetic Characterization of Puccinia graminis f.sp. tritici Populations from Ethiopia by SSRs

Admassu

Friedt

Ordon

2010

Journal of Phytopathology

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The genetic structure of Puccinia graminis f.sp. tritici isolates sampled in three different regions of Ethiopia in 2006 and 2007 was investigated using 20 SSRs assuming sexual as well as asexual reproduction. Assays showed a high level of genetic diversity within P. graminis f.sp. tritici populations in Ethiopia in both modes of reproductions. Tests for population subdivision revealed the absence of genetic differentiation among the populations based on geographical separation as reflected by a low coefficient of genetic differentiation (≤0.046) and a single cluster consisting of all isolates. Gene flow among populations was estimated to be high (10 per generation). This study shows that the pathogen population of Ethiopia is characterized by a high genetic diversity within each population and absence of segregation among populations. Information obtained from this study may serve as a basis to develop better strategies for deployment of resistance genes, e.g. using marker‐assisted combination of resistance alleles to achieve better control of wheat stem rust in Ethiopia.

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Section: Discussionsupporting

confidence: 60%

Genetic Characterization of Puccinia graminis f.sp. tritici Populations from Ethiopia by SSRs

Admassu

Friedt

Ordon

2010

Journal of Phytopathology

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“…triticina and Melampsora larici‐populina . These fungi also have an efficient mechanism for dissemination over long distances (Hurtado & Ramstedt, ; Hamelin et al ., ; Mebrate et al ., ; Pei et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and structure of Hemileia vastatrix populations on Coffea spp.

et al. 2015

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Coffee leaf rust is the most limiting disease for coffee cultivation in Brazil. Despite its importance, relatively little is known about the genetic diversity of Hemileia vastatrix, the rust causal agent. In this work, the DNA from 112 monopustule isolates from different geographic locations and coffee genotypes were analysed by amplified fragment length polymorphisms (AFLP). The objectives were to assess the influence of the host and geographic origin on the diversity and population differentiation in H. vastatrix. The fungal population showed a low level of genotypic diversity. Gene diversity (h) was 0·027 and the hypothesis of random mating in the total population was rejected, but evidence for recombination was found for two subpopulations (São Paulo and Paraná). The analysis of molecular variance revealed that 90% of the genetic distribution of the pathogen occurs among isolates within the subpopulation (states or host of origin). There was no correlation between geographic and genetic distance (r = −0·024, P = 0·74), which together with the high number of migrants and the low degree of differentiation in populations of H. vastatrix, is consistent with the fact that the inoculum is probably easily dispersed by wind over long distances, allowing dispersal of the pathogen among coffee growing areas in Brazil. Therefore, it is difficult to predict the durability of resistant sources to coffee rust. The recommendation for the breeding programmes is thus to incorporate multigenic resistance as a control strategy.

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“…Furthermore, these isolates were tested for virulence on leaves of the accessions PI272560 and 36554. Isolates were stored and spores were produced according to the method of Mebrate et al (2006). Position of pots were randomized and changed daily to avoid possible effects of location.…”

Section: Methodsmentioning

confidence: 99%

Microscopic and Molecular Characterization of the Prehaustorial Resistance against Wheat Leaf Rust (Puccinia triticina) in Einkorn (Triticum monococcum)

et al. 2016

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Puccinia triticina f. sp. tritici (Eriks.), the causal agent of leaf rust, causes substantial yield losses in wheat production. In wheat many major leaf rust resistance genes have been overcome by virulent races. In contrast, the prehaustorial resistance (phr) against wheat leaf rust detected in the diploid wheat Einkorn (Triticum monoccocum var. monococcum) accession PI272560 confers race-independent resistance against isolates virulent on accessions harboring resistance genes located on the A-genome of Triticum aestivum. Phr in PI272560 leads to abortion of fungal development during the formation of haustorial mother cells and to increased hydrogen peroxide concentration in comparison to the susceptible accession 36554 (Triticum boeoticum ssp. thaoudar var. reuteri). Increased peroxidase and endochitinase activity was detected in PI272560 within 6 h after inoculation (hai). Comparative transcriptome profiling using Massive Analysis of cDNA Ends (MACE) in infected and non-infected leaves detected 14220 differentially expressed tags in PI272560 and 15472 in accession 36554. Of these 2908 and 3004, respectively, could be assigned to Gene Ontology (GO) categories of which 463 were detected in both accessions and 311 were differentially expressed between the accessions. In accordance with the concept of non-host resistance in PI272560, genes with similarity to peroxidases, chitinases, β-1,3-glucanases and other pathogenesis-related genes were up-regulated within the first 8 hai, whereas up-regulation of such genes was delayed in 36554. Moreover, a Phosphoribulokinase gene contributing to non-host resistance in rice against stripe rust was exclusively expressed in the resistant accession PI272560. Gene expression underpinned physiological and phenotypic observations at the site of infection and are in accordance with the concept of non-host resistance.

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Molecular Diversity in Puccinia triticina Isolates from Ethiopia and Germany

Cited by 20 publications

References 39 publications

Genetic Characterization of Puccinia graminis f.sp. tritici Populations from Ethiopia by SSRs

Genetic Characterization of Puccinia graminis f.sp. tritici Populations from Ethiopia by SSRs

Genetic diversity and structure of Hemileia vastatrix populations on Coffea spp.

Microscopic and Molecular Characterization of the Prehaustorial Resistance against Wheat Leaf Rust (Puccinia triticina) in Einkorn (Triticum monococcum)

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