Molecular Diversity of Compounds from Pygidial Gland Secretions of Cave-Dwelling Ground Beetles: The First Evidence

Vesović, Nikola; Ćurčić, Srećko; Vujisić, Ljubodrag; Nenadić, Marija; Krstić, Gordana; Perić-Mataruga, Vesna; Milosavljević, Slobodan; Antić, Dragan Ž.; Mandić, Boris; Petković, Matija; Vučković, Ivan; Marković, Đorđe; Vrbica, Maja; Ćurčić, B.P.M.; Makarov, Slobodan E.

doi:10.1007/s10886-015-0593-7

Cited by 18 publications

(29 citation statements)

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“…The pygidial gland secretions of ground beetles are chiefly composed of organic acids, aldehydes, alcohols, and terpenes (Eisner et al 1977;Moore 1979;Dazzini-Valcurone and Pavan 1980;Dettner 1987;Giglio et al 2011). Previous investigators determined the chemical composition of pygidial gland secretions of the ground beetle species Carabus ullrichii Germar, 1824; C. coriaceus L., 1758; Abax parallelepipedus (Piller & Mitterpacher, 1783); and Laemostenus punctatus (Dejean, 1828) (Lečić et al 2014;Vesović et al 2015Vesović et al , 2017. According to their analyses, C. ullrichii produces the following acids: methacrylic (78.7%); angelic (17.7%); tiglic (2.5%); and benzoic, isobutyric, butyric, and 2-methyl butyric (less than 1% each) (Lečić et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Abax parallelepipedus synthesizes the following acids: methacrylic (76.5%); tiglic (22.9%); and isobutyric, crotonic, and senecioic (less than 1% each) (Lečić et al 2014). Finally, L. punctatus releases undecane (40.4%); dodecyl acetate (34.2%); formic acid (19.4%); 9-methyltetracosane (2.6%); dodecan-1-ol (1.2%); and acetic acid, 7-hexyldocosane, palmitic acid, decyl acetate, and undecyl acetate, as well as stearic, oleic, and caproic acids (less than 1% each) (Vesović et al 2015). Nenadić et al (2016aNenadić et al ( , b, 2017 recorded that pygidial gland secretions of the mentioned ground beetle species possess an antimicrobial potential against human pathogens and pathogens in the environment.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae)

et al. 2018

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Inhibition of the proliferation of human tumour cells and porcine non-tumour cells by the pygidial gland secretion released by adults of four ground beetle species was observed in this study. The sulphorhodamine B (SRB) assay was applied to establish the percentages of inhibition of the net growth of four human tumour cell lines and porcine liver primary non-tumour cells. The secretions of all tested ground beetle species were shown to have an antiproliferative effect on the tested cell lines. Special emphasis is put on the secretion of Abax parallelepipedus, which showed the highest antitumour potential and weakest inhibition of non-tumour cell proliferation. The antitumour and antiproliferative potential of the pygidial gland secretions of ground beetles is here demonstrated for the first time. It is suggested that certain organic acids are responsible for the action. Further investigation needs to be conducted in order to better understand the mechanisms governing the observed cytotoxic and antitumour activity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae)

et al. 2018

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“…Many of these arthropods are chemically still well-defended, and chemical defense appears to persist when species become cavernicolous. The defensive chemistry of troglobionts, however, has only been elucidated for a few species, mainly for some polydesmid millipedes, one example of cave-harvestmen and two carabids (Makarov et al 2012; Shear et al 2010a, 2010b; Vesović et al 2015). A possible cave-effect on chemical defense, in terms of modification of defensive chemistry compared to non-cave dwellers of the same taxonomic group, has never been investigated in detail.…”

Section: Introductionmentioning

confidence: 99%

Chemical Ecology of Cave-Dwelling Millipedes: Defensive Secretions of the Typhloiulini (Diplopoda, Julida, Julidae)

et al. 2017

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Cave animals live under highly constant ecological conditions and in permanent darkness, and many evolutionary adaptations of cave-dwellers have been triggered by their specific environment. A similar “cave effect” leading to pronounced chemical interactions under such conditions may be assumed, but the chemoecology of troglobionts is mostly unknown. We investigated the defensive chemistry of a largely cave-dwelling julid group, the controversial tribe “Typhloiulini”, and we included some cave-dwelling and some endogean representatives. While chemical defense in juliform diplopods is known to be highly uniform, and mainly based on methyl- and methoxy-substituted benzoquinones, the defensive secretions of typhloiulines contained ethyl-benzoquinones and related compounds. Interestingly, ethyl-benzoquinones were found in some, but not all cave-dwelling typhloiulines, and some non-cave dwellers also contained these compounds. On the other hand, ethyl-benzoquinones were not detected in troglobiont nor in endogean typhloiuline outgroups. In order to explain the taxonomic pattern of ethyl-benzoquinone occurrence, and to unravel whether a cave-effect triggered ethyl-benzoquinone evolution, we classed the “Typhloiulini” investigated here within a phylogenetic framework of julid taxa, and traced the evolutionary history of ethyl-benzoquinones in typhloiulines in relation to cave-dwelling. The results indicated a cave-independent evolution of ethyl-substituted benzoquinones, indicating the absence of a “cave effect” on the secretions of troglobiont Typhloiulini. Ethyl-benzoquinones probably evolved early in an epi- or endogean ancestor of a clade including several, but not all Typhloiulus (basically comprising a taxonomic entity known as “Typhloiulus sensu stricto”) and Serboiulus. Ethyl-benzoquinones are proposed as novel and valuable chemical characters for julid systematics.Electronic supplementary materialThe online version of this article (doi:10.1007/s10886-017-0832-1) contains supplementary material, which is available to authorized users.

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“…Our results demonstrate that the ground beetle species L. (P.) punctatus produces pygidial gland secretion, which, apart from the assumed defensive role (Vesović et al, 2015), has both antibacterial and antifungal properties as well.…”

Section: Discussionmentioning

confidence: 75%

“…Table 1. Components of the pygidial gland secretion of L. (P.) punctatus and the volume in the secretion (modified after Vesović et al, 2015).…”

Section: Morphology Of the Pygidial Glands Of L (P) Punctatusmentioning

confidence: 99%

Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetleLaemostenus(Pristonychus)punctatus(Dejean, 1828) (Insecta: Coleoptera: Carabidae)

Nenadić

Sokóvić

Glamočlija

et al. 2016

Bull. Entomol. Res.

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The antimicrobial activity of the pygidial gland secretion released by adult individuals of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828), applying microdilution method with the aim to detect minimal inhibitory concentration, minimal bactericidal concentration and minimal fungicidal concentration, has been investigated. In addition, morphology of the pygidial glands is observed. We have tested 16 laboratory and clinical strains of human pathogens - eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples have showed antimicrobial properties against all strains of treated bacteria and fungi. Micrococcus flavus proved to be more resistant compared with other bacterial strains. More significant antimicrobial properties of the secretion are observed against Escherichia coli, which proved to be the most sensitive bacteria. Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and Penicillium verrucosum var. cyclopium the most sensitive micromycetes. Commercial antibiotics Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied as positive controls, showed higher antibacterial properties for all bacterial and fungal strains, except for P. ochrochloron, which proved to be more resistant on Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart from the role in ecological aspects, the antimicrobial properties of the tested secretion possibly might have medical significance in the future.

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Molecular Diversity of Compounds from Pygidial Gland Secretions of Cave-Dwelling Ground Beetles: The First Evidence

Cited by 18 publications

References 12 publications

Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae)

Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae)

Chemical Ecology of Cave-Dwelling Millipedes: Defensive Secretions of the Typhloiulini (Diplopoda, Julida, Julidae)

Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetleLaemostenus(Pristonychus)punctatus(Dejean, 1828) (Insecta: Coleoptera: Carabidae)

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