Molecular Diversity of Phytoplasmas Associated with Grapevine Yellows Disease in North-Eastern Italy

Zambon, Yuri; Canel, A.; Bertaccini, Assunta; Contaldo, Nicoletta

doi:10.1094/phyto-07-17-0253-r

Cited by 21 publications

(22 citation statements)

References 49 publications

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“…"Ca. P. trifolii" is also prevalent pathogen for various hosts in Asia, Europe and, American continents, including tomato in Jordan (Anfoka et al, 2003), pepper in Mexico (Reveles-Torres et al, 2018), grapevine, tomato, soybean, safflower in Iran (Shahryari et al, 2019;Ghayeb Zamharir and Aldaghi, 2018;Salehi et al, 2008;Davoodi et al, 2019), cabbage, sesame, periwinkle, eggplant, pepper in Turkey (Oksal et al, 2017;Ulubaş Serçe and Yılmaz, 2019;Özdemir, 2017;Sertkaya et al, 2007), in American Elm (Ulmus americana L.) in U.S.A (Flower et al, 2018), willow in China (Zhang et al, 2012), grapevine in Italy (Zambon et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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Phytoplasma-type symptoms were noted in tomato (Lycopersicum esculentum L.) in Bingöl province of Turkey. The remarkable symptoms include witch's broom, rosetting, purple curled and crispy leaves, small leaves in the upper branches and excessively elongated calyx. Genomic DNA isolation was performed to identify possible pathogens from the leaves of 11 plants with and without symptom. In the Nested-PCR test performed using universal primer sets, DNA bands of approximately 1200 bp size were obtained in 4 of 11 samples. Randomly chosen two DNA bands primed R16F2n/R16R2 were cloned into an appropriate plasmid vector to further characterizations. The recombinant plasmid DNAs purified were sequenced in both directions. Molecular assays of the 16S rRNA sequence confirmed the existence of the "Canditatus Phytoplasma solani" (16SrXII-A group) (similarity coefficient 1.00) (Accession no: MT279680) and the "Canditatus Phytoplasma trifolii" (16SrVI-A group) (similarity coefficient 1.00) (Accession no: MT279852) in the infected tomato samples. The isolates associated with tomato-phytoplasma were named as 'Bingöl D11' and 'Bingöl D90' isolates, respectively. The phylogenetic dendrogram created also confirmed where both pathogens belong. This current paper is documented in the first record of "Ca. P. solani" (16SrXII-A) and "Ca. P. trifolii" (16SrVI-A) in naturally diseased tomato in Bingöl of Turkey.

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Section: Discussionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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“…The main uncertainties of this listing concern the geographic distribution, taxonomy, biology and host range of some phytoplasmas. Species description: (Griffiths et al, 1999); Fragaria: (Fernandez et al, 2013); Prunus: (Zunnoon-Khan et al, 2010); Vitis: (Gajardo et al, 2009;Ghayeb Zamharir et al, 2017;Zambon et al, 2018); Italy: (Bruni et al, 2005;Zambon et al, 2018) 4 Candidatus Phytoplasma hispanicum…”

Section: Discussionmentioning

confidence: 99%

List of non‐EU phytoplasmas of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L. and Vitis L.

Bragard¹,

Dehnen‐Schmutz²,

Gonthier³

et al. 2020

EFS2

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Following a request from the European Commission, the EFSA Panel on Plant Health prepared a list of non‐EU phytoplasmas of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L. and Vitis L. A systematic literature review and search of databases identified 27 phytoplasmas infecting one or more of the host genera under consideration. These phytoplasmas were assigned to three categories. The first group (a) consists of 10 non‐EU phytoplasmas, known to occur only outside the EU (‘Candidatus Phytoplasma australiense’, ‘Ca. P. hispanicum’, ‘Ca. P. pruni’‐related strain (NAGYIII), ‘Ca. P. pyri’‐related strain (PYLR) and Buckland valley grapevine yellows phytoplasma) or having only limited presence in the EU (‘Ca. P. aurantifolia’‐related strains, ‘Ca. P. fraxini’, ‘Ca. P. phoenicium’, ‘Ca. P. trifolii’ and ‘Ca. P. ziziphi’). The second group (b) consists of three non‐EU phytoplasmas, whose presence in the target plant species is not fully supported by the available literature. The third group (c) consists of 14 phytoplasmas with substantial presence in the EU (i.e. they are originally described or reported from the EU or known to occur or be widespread in some EU Member States or frequently reported in the EU). Phytoplasmas of categories (b) and (c) were excluded at this stage from further categorisation efforts. One phytoplasma from category (a) (‘Ca. P. phoenicium’) was excluded from further categorisation, as a pest risk assessment has been performed by EPPO. Comments provided by the EU Member States were integrated in the opinion. The main uncertainties of this listing concern: the geographic distribution and prevalence, the taxonomy, biology and host range. The phytoplasmas considered as non‐EU and whose presence in target plant species is fully supported by literature (category (a)) are categorised by the Panel in a separate opinion.

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“…It is also important to constantly examine both the asymptomatic and symptomatic plants, alternative host plants in the vineyards and in the surrounding areas, and insect vectors, as new variants of the phytoplasma are constantly emerging. For example, Zambon et al (2018) detected new strains of phytoplasmas from the main viticulture areas of the northern Italy from both grapevine and insect vector, indicating the potential of the pathogen to evolve, spread, and further cause damage to the new vineyard areas. Indeed, disease prevention and control should also consider the potential role of phytoplasma strains belonging to the 16SrVI and 16SVII-A associated with emerging diseases in grapevine in Italy, China, Syria, and Iran (Zambon et al, 2018), opening new possible epidemiological patterns in vineyard ecosystems.…”

Section: Discussionmentioning

confidence: 99%

“…For example, Zambon et al (2018) detected new strains of phytoplasmas from the main viticulture areas of the northern Italy from both grapevine and insect vector, indicating the potential of the pathogen to evolve, spread, and further cause damage to the new vineyard areas. Indeed, disease prevention and control should also consider the potential role of phytoplasma strains belonging to the 16SrVI and 16SVII-A associated with emerging diseases in grapevine in Italy, China, Syria, and Iran (Zambon et al, 2018), opening new possible epidemiological patterns in vineyard ecosystems. In this contest, pest monitoring is a prerequisite for effective decision making in integrated pest management programs.…”

Section: Discussionmentioning

confidence: 99%

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The Distribution of Phytoplasmas in South and East Asia: An Emerging Threat to Grapevine Cultivation

et al. 2019

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Grapevine is largely cultivated in several parts of the world, and a spurt in its cultivation has occurred in the last two decades in grapevine cultivated areas of South and East Asia, mainly in China, India, Japan, Korea, Thailand, and Indonesia. Grapevine yellows (GY) represent one of the most important diseases in viticultural areas of the world, and they have been assigned to five different groups: aster yellows [AY (16SrI)], peanut witches’ broom [PnWB (16SrII)], X-disease (16SrIII), elm yellows [EY (16SrV)], and Stolbur (16SrXII). This study provides a comprehensive overview of the presence of phytoplasma strains and their vectors associated with GY complex, and their potential impact on viticulture of the South and East Asia. In general, both AY and EY were reported on several herbaceous plants and/or cultivated plants in South and East Asia, along with its vectors that were largely reported in China and sporadically in Japan. Interestingly, AY and EY are yet not found in South and East Asia grapevine regions; however, their presence on different plant species suggests the potential spread of the pathogens that may occur in grapevine regions in the near future. Additionally, a few reports also suggest the presence of Stolbur group in Asian countries, along with one study that found a Stolbur-related strain in China on Vitis vinifera. Similarly, PnWB was also frequently reported in India and China on several plant species, but not in grapes. Conversely, sporadic detections of phytoplasma strains related to X-disease in Thailand, South Korea, and China indicate that their potential influence in viticulture is rather negligible. Our review suggests that monitoring and control strategies against GY are essential in order to prevent epidemic phytoplasma spread, especially in vine-allocated areas in Asia.

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Molecular Diversity of Phytoplasmas Associated with Grapevine Yellows Disease in North-Eastern Italy

Cited by 21 publications

References 49 publications

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

List of non‐EU phytoplasmas of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L. and Vitis L.

The Distribution of Phytoplasmas in South and East Asia: An Emerging Threat to Grapevine Cultivation

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