Molecular Diversity of Rhizobacteria in Ginseng Soil and Their Plant Benefiting Attributes

Hong, Eun Hye; Lee, Sun Hee; Vendan, R. Thamizh; Rhee, Young Ha

doi:10.7845/kjm.2012.047

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…To date, several studies have attempted to investigate the resident bacterial communities in ginseng soil. For example, rhizobacteria belonging to the phyla Actinobacteria and Firmicutes were isolated from 1- to 3-year-old ginseng rhizosphere soil samples using a culture-dependent method [ 10 ]. However, small factions of bacterial populations exist in soil can be cultured in laboratory.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Diversity and Community Structure in Korean Ginseng Field Soil Are Shifted by Cultivation Time

Nguyen¹,

Kim²,

Hoang³

et al. 2016

PLoS ONE

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Traditional molecular methods have been used to examine bacterial communities in ginseng-cultivated soil samples in a time-dependent manner. Despite these efforts, our understanding of the bacterial community is still inadequate. Therefore, in this study, a high-throughput sequencing approach was employed to investigate bacterial diversity in various ginseng field soil samples over cultivation times of 2, 4, and 6 years in the first and second rounds of cultivation. We used non-cultivated soil samples to perform a comparative study. Moreover, this study assessed changes in the bacterial community associated with soil depth and the health state of the ginseng. Bacterial richness decreased through years of cultivation. This study detected differences in relative abundance of bacterial populations between the first and second rounds of cultivation, years of cultivation, and health states of ginseng. These bacterial populations were mainly distributed in the classes Acidobacteria, Alphaproteobacteria, Deltaproteobacteria, Gammaproteobacteria, and Sphingobacteria. In addition, we found that pH, available phosphorus, and exchangeable Ca+ seemed to have high correlations with bacterial class in ginseng cultivated soil.

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Section: Introductionmentioning

confidence: 99%

Bacterial Diversity and Community Structure in Korean Ginseng Field Soil Are Shifted by Cultivation Time

Nguyen¹,

Kim²,

Hoang³

et al. 2016

PLoS ONE

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“…The rhizospheric region of ginseng roots is enhanced with beneficiary micro-organisms (Hong et al , 2012; Li et al , 2012). Pseudomonas fluorescens RAF15 was isolated from ginseng rhizophere soils and showed plant-growth-promoting and antifungal activities against phytopathogenic fungi (Park et al , 2009).…”

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confidence: 99%

Epilithonimonas ginsengisoli sp. nov., isolated from soil of a ginseng field

Hoang

Kim

Ponnuraj

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-staining-negative, rod-shaped bacterium, designated DCY78 T , was isolated from soil of a ginseng field in Yeon-cheon province (386 049 000 N 1266 579 000 E), Republic of Korea. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain DCY78 T belonged to the genus Epilithonimonas and was most closely related to Epilithonimonas lactis DSM 19921 T (98.5 % sequence similarity) and Epilithonimonas tenax DSM 16811 T (97.8 %). Growth occurred at 10-30 6C with an optimum temperature of 28 6C. The pH range for growth was pH 5.5-8.0. The major polar lipids were found to be phosphatidylethanolamine three unidentified amino lipids and one unidentified polar lipid. The only predominant quinone was MK-6. The major polyamines were sym-homospermidine and spermidine. The major fatty acids were summed feature 3 (comprising C 16 : 1 v6c and/or C 16 : 1 v7c), iso-C 15 : 0 and iso-C 17 : 0 3-OH. The DNA G+C content was 37.9 mol%. On the basis of the phenotypic and genotypic analysis, the isolate is classified as representative of a novel species in the genus Epilithonimonas, for which the name Epilithonimonas ginsengisoli is proposed. The type strain is DCY78 T (5KCTC 32174 T 5JCM 19896 T ).

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Comparative study of rhizobacterial communities in pepper greenhouses and examination of the effects of salt accumulation under different cropping systems

et al. 2016

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This study compared rhizobacterial communities in pepper greenhouses under a paddy-upland (rice-pepper) rotational system (PURS) and a monoculture repeated cropping system (RCS) and examined adverse effects of high salinity on soil properties. The following soil properties were analyzed: electrical conductivity (EC), pH, concentration of four cations (Na, Ca, Mg, and K), total nitrogen, and organic matter content. Rhizobacterial communities were analyzed using culture-based and culture-independent (pyrosequencing) methods. In addition, all culturable bacteria isolated from each soil sample were tested for traits related to plant growth promotion. The EC of rhizospheric soils was 5.32-5.54 dS/m for the RCS and 2.05-2.19 dS/m for the PURS. The culture-based method indicated that the bacterial communities and bacterial characteristics were significantly more diverse in the PURS soil than in the RCS soil. The pyrosequencing data also indicated that the richness and diversity of bacterial communities were greater in the PURS soil. Proteobacteria was the most abundant phylum in soil samples under both cropping systems. However, Firmicutes and Gemmatimonadetes were more prevalent in the RCS soil, while the PURS soil contained a greater number of Chloroflexi. Spearman's correlation coefficients showed that soil EC was significantly positively correlated with the abundance of Firmicutes and Gemmatimonadetes and negatively correlated with the abundance of Acidobacteria, Chloroflexi, and Deltaproteobacteria. This is the first study on the rhizobacterial communities in pepper greenhouses under two different cropping systems using both culture- and pyrosequencing-based methods.

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Molecular Diversity of Rhizobacteria in Ginseng Soil and Their Plant Benefiting Attributes

Cited by 3 publications

References 29 publications

Bacterial Diversity and Community Structure in Korean Ginseng Field Soil Are Shifted by Cultivation Time

Bacterial Diversity and Community Structure in Korean Ginseng Field Soil Are Shifted by Cultivation Time

Epilithonimonas ginsengisoli sp. nov., isolated from soil of a ginseng field

Comparative study of rhizobacterial communities in pepper greenhouses and examination of the effects of salt accumulation under different cropping systems

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