Molecular Diversity Studies in Lake Pavin Reveal the Ecological Importance of Parasitic True Fungi in the Plankton

Sime-Ngando, Télesphore; Gerphagnon, Mélanie; Colombet, Jonathan; Jobard, Marlène; Lefèvre, Emilie; Monchy, Sébastien; Rasconi, Séréna; Latour, Delphine; Carrias, Jean‐François; Amblard, Christian

doi:10.1007/978-3-319-39961-4_20

Cited by 4 publications

(3 citation statements)

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“…Among affected members, As. formosa is a diatom that blooms in Lake Pavin and a host for the chytrid parasite (Sime‐Ngando, Gerphagnon, et al, 2016 ). Although chytrid infection in Aulacoseira has not been reported in Lake Pavin, previous studies have shown that Aulacoseira could be a host for parasitic chytrids (Kagami et al, 2012 ; Maier & Peterson, 2014 ; Seto & Degawa, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

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Taxonomic and functional dynamics during chytrid epidemics in an aquatic ecosystem

Delgado‐Viscogliosi

Gerphagnon

et al. 2022

Molecular Ecology

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Section: Discussionmentioning

confidence: 99%

“…As. formosa is a diatom that blooms in Lake Pavin and a host for the chytrid parasite (Sime-Ngando, Gerphagnon, et al, 2016). Although chytrid infection in Aulacoseira has not been reported in Lake…”

Section: Dynamics In Eukaryotic Microbial Biodiversitymentioning

confidence: 99%

Taxonomic and functional dynamics during chytrid epidemics in an aquatic ecosystem

Delgado‐Viscogliosi

Gerphagnon

et al. 2022

Molecular Ecology

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“…KEYWORDS biobanking, bloom dynamics, Chytridiomycota, cryopreservation, molecular methods, pathosystem, phytopathogens, phytoplankton F ungi belonging to the phylum Chytridiomycota (i.e., chytrids) are important parasites of freshwater phytoplankton (1). Their multifaceted contribution to food web dynamics is increasingly recognized (2,3): chytrid infections have been shown to be one of the main factors controlling the density and genetic structure of their host population (4)(5)(6), with a huge impact on the succession of phytoplankton species and the entire trophic food web (7). In particular, chytrid infections of phytoplankton drive the mycoloop, a trophic shortcut that facilitates the transfer of organic carbon and key nutrients from inedible phytoplankton to higher trophic levels (2,(8)(9)(10).…”

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confidence: 99%

Rediscovering Zygorhizidium affluens Canter: Molecular Taxonomy, Infectious Cycle, and Cryopreservation of a Chytrid Infecting the Bloom-Forming Diatom Asterionella formosa

Rad‐Menéndez

Gerphagnon

Garvetto

et al. 2018

Appl Environ Microbiol

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Parasitic Chytridiomycota (chytrids) are ecologically significant in various aquatic ecosystems, notably through their roles in controlling bloom-forming phytoplankton populations and in facilitating the transfer of nutrients from inedible algae to higher trophic levels. The diversity and study of these obligate parasites, while critical to understand the interactions between pathogens and their hosts in the environment, have been hindered by challenges inherent to their isolation and stable long-term maintenance under laboratory conditions. Here, we isolated an obligate chytrid parasite (CCAP 4086/1) on the freshwater bloom-forming diatom Asterionella formosa and characterized its infectious cycle under controlled conditions. Phylogenetic analyses based on 18S, 5.8S, and 28S ribosomal DNAs (rDNAs) revealed that this strain belongs to the recently described clade SW-I within the Lobulomycetales. All morphological features observed agree with the description of the known Asterionella parasite Zygorhizidium affluens Canter. We thus provide a phylogenetic placement for this chytrid and present a robust and simple assay that assesses both the infection success and the viability of the host. We also validate a cryopreservation method for stable and cost-effective long-term storage and demonstrate its recovery after thawing. All the above-mentioned tools establish a new gold standard for the isolation and long-term preservation of parasitic aquatic chytrids, thus opening new perspectives to investigate the diversity of these organisms and their physiology in a controlled laboratory environment. IMPORTANCE Despite their ecological relevance, parasitic aquatic chytrids are understudied, especially due to the challenges associated with their isolation and maintenance in culture. Here we isolated and established a culture of a chytrid parasite infecting the bloom-forming freshwater diatom Asterionella formosa. The chytrid morphology suggests that it corresponds to the Asterionella parasite known as Zygorhizidium affluens. The phylogenetic reconstruction in the present study supports the hypothesis that our Z. affluens isolate belongs to the order Lobulomycetales and clusters within the novel clade SW-I. We also validate a cryopreservation method for stable and cost-effective long-term storage of parasitic chytrids of phytoplankton. The establishment of a monoclonal pathosystem in culture and its successful cryopreservation opens the way to further investigate this ecologically relevant parasitic interaction.

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Seeökosysteme IV: Populationsökologie ‐ Dichte, Wachstum, Reproduktion und Mortalität der Populationen (Teil 1)

Geller¹,

Hupfer

2018

Handbuch Angewandte Limnologie: Grundlagen ‐ Gewässerbelastung ‐ Restaurierung ‐ Aquatische Ökotoxikologie ‐ Bewertung ‐ Gewäss

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Molecular Diversity Studies in Lake Pavin Reveal the Ecological Importance of Parasitic True Fungi in the Plankton

Cited by 4 publications

References 77 publications

Taxonomic and functional dynamics during chytrid epidemics in an aquatic ecosystem

Taxonomic and functional dynamics during chytrid epidemics in an aquatic ecosystem

Rediscovering Zygorhizidium affluens Canter: Molecular Taxonomy, Infectious Cycle, and Cryopreservation of a Chytrid Infecting the Bloom-Forming Diatom Asterionella formosa

Seeökosysteme IV: Populationsökologie ‐ Dichte, Wachstum, Reproduktion und Mortalität der Populationen (Teil 1)

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