Molecular docking analyses of Escin as regards cyclophosphamide-induced cardiotoxicity: In vivo and in Silico studies

Gür, Fatma; Cengiz, Mustafa; Kutlu, Hatice Mehtap; Cengiz, Betül; Ayhancı, Adnan

doi:10.1016/j.taap.2020.115386

Cited by 25 publications

(10 citation statements)

References 24 publications

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“…In this study, CP caused liver damage, which increased the levels of ALP, AST, MDA, and MPO, and decreased the levels of SOD, CAT, GSH‐Px, and LDH (Ayhanci et al, 2019). Gür et al reported that the interactions of CP with the catalytic residues of CAT (Arg71, Phe333, and Tyr357) reduced the enzymatic activity of CAT by limiting the mobility and conformation of this region (Gür et al, 2021). In the histopathology of the liver, CP obviously damaged the hepatic lobules and hepatic cord of the liver in mice.…”

Section: Discussionmentioning

confidence: 99%

Prevention effect of total ginsenosides and ginseng extract from Panax ginseng on cyclophosphamide‐induced immunosuppression in mice

Zhang

Sun

Fan

et al. 2023

Phytotherapy Research

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Oral decoction is widely applied in traditional Chinese medicines. The polysaccharides of decoction promote the exposure of small molecules and increase their bioavailability. This study mainly compared the component and activities of total ginsenosides (TGS) and ginseng extract (GE) on immunosuppressed mice induced by cyclophosphamide. Thirty‐two mice were randomly divided into control, model, TGS, and GE groups. The mice were orally administered for 28 days and then injected with cyclophosphamide on the last four days. The results of component analysis showed the total content of 12 ginsenosides in TGS (67.21%) was higher than GE (2.04%); the total content of 17 amino acids in TGS (1.41%) was lower than GE (5.36%); the total content of 10 monosaccharides was similar in TGS (74.12%) and GE (76.36%). The animal results showed that both TGS and GE protected the hematopoietic function of bone marrow by inhibiting cell apoptosis, and recovering the normal cell cycle of BM; maintained the dynamic balance between the Th1 and Th2 cells; also protected the spleen, thymus, and liver. Meanwhile, TGS and GE protected the intestinal bacteria of immunosuppressed mice by increasing the abundance of lactobacillus and decreasing the abundance of the odoribacter and clostridia_UCG‐014. The prevention effect of GE was superior to TGS in some parameters. In conclusion, TGS and GE protected the immune function of immunosuppressed mice induced by cyclophosphamide. Meanwhile, GE showed higher bioavailability and bioactivity compared with TGS, because the synergistic effect of polysaccharides and ginsenosides plays an important role in protecting the immune function.

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Section: Discussionmentioning

confidence: 99%

Prevention effect of total ginsenosides and ginseng extract from Panax ginseng on cyclophosphamide‐induced immunosuppression in mice

Zhang

Sun

Fan

et al. 2023

Phytotherapy Research

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“…However, the addition of HT increased the negative binding energy value from − 5.0 kcal/mol to -4.8 kcal/mol due to the formation of the CP/Caspase-3 complex besides increasing the average length of three hydrogen bonds that formed between CP and Arg207 increased. The fact that the negative binding energy value, an indication of the a nity of the protein to ligand, calculated after a co-application of CP-HT had increased when compared to that of the CP-alone meant that the a nity of Caspase-3 to CP had also decreased (Canofeni et al, 1994r, 2020Gür, Cengiz, Kutlu, et al, 2021;Kassa et al, 2022). In other words, these results are suggestive of decreased Caspase-3 expression.…”

Section: Discussionmentioning

confidence: 99%

“…3,Bax, and Bcl-2 were performed thanks to the PyRx program(Gür, 2020;Gür, Cengiz, Kutlu, et al, 2021;Trott & Olson, 2010). The energy minimization and the other parameters were set as done in our previous studiesGür et al, 2020).…”

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confidence: 99%

Molecular docking analysis and anti-apoptotic activity of Hypericum triquetrifolium Turra (St. John's-wort) in Cyclophosphamide-induced lung injury

Yıldız

Keskin

Şahıntürk

et al. 2022

Preprint

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The present study aims to investigate the lung-protective and antiapoptotic effects of Hypericum triquetrifolium Turra (HTT) against cyclophosphamide (CP)-induced lung injury in rats. Thirty-five Sprague Dawley rats were categorized into 5 groups, each consisting of seven members. Phenolic acid and flavonoid contents of this plant were determined. The lung tissue samples cultivated from the rats were examined both histopathological and immunohistochemically for the apoptosis markers of Caspase-3, Bax, and Bcl-2. Histopathological results indicated that structural defects, bleeding areas, and edema had occurred in the lungs of the CP-Alone Group. Besides, Caspase-3 and Bax positivity of the lung cells had also increased while Bcl-2 positivity had decreased. On the other hand, in the HTT + CP Group, HTT was shown to have reversed the aforementioned changes positively. In addition, our in-vivo results were confirmed by the in-silico studies. The changes that occurred in the binding of CP to the active-site amino acid residues of Caspase-3, Bax, and Bcl-2 upon the addition of Hyperoside besides the changes that occurred in their tendency to form hydrogen bonds were accounted for by in silico studies. Based on in vivo and in silico results, HTT could be a strong protective candidate for CP-induced lung injury and apoptosis.

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“…It has been demonstrated that the genus Salvia L., known as adaçayı in Turkey, has many traditional uses, both internally and externally, among which are the digestive system (as an appetizer, gas reliever, and stomach smoother), the respiratory system (as a cough suppressant, and bronchitis and asthma reliever), and the immune system (as a protective agent against infections and colds, an antiseptic agent, and a wound-healer) (Cengiz et al, 2016;Cadirci et al, 2012). Previous studies have reported that besides having antioxidant (Ucuncu et al, 2004;Yoruk et al, 2009), anti-tumor, immunomodulatory, anti-inflammatory, and anti-viral activities, SV is used in treating hemorrhoids (Gür et al, 2021;Tepe, 2008;Furtado et al, 2010). The present study aims to investigate possible cytoprotective effects of Salvia virgata on peripheral blood and bone marrow nucleated cells in order to eliminate the dose-limiting side effects of CP, a powerful antitumor agent, thus enabling this drug to be safely used in higher doses.…”

Section: Introductionmentioning

confidence: 99%

Siklofosfamid Kaynaklı Toksisite Üzerine Salvia virgata Jacq'ın Hematoprotektif ve Antioksidan Etkilerinin Araştırılması

Şahin

2022

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Bu çalışma, Salvia virgata'nın (SV) Siklofosfamid (CP) kaynaklı toksisite üzerindeki hematoprotektif ve antioksidan etkilerini araştırmayı amaçlamaktadır. Sıçanlar, her biri 7 üyeden oluşan 6 gruba ayrıldı. Bunlar Kontrol grubu, CP Grubu (150 mg kg -1 ), 100 ve 200 mg kg -1 SV Grupları ve CP+100 ve CP+200 mg kg -1 SV Gruplarıdır. Tüm sıçanlar, son enjeksiyonlardan sonraki gün kan ve kemik iliği numunelerini toplamak için kurban edildi. Kan örneklerinin bir kısmı lökosit ve trombosit sayımı için kullanılırken, diğerleri toplam antioksidan kapasite (TAC), malondialdehit (MDA), toplam oksidan durumu (TOS), glutatyon (GSH) ve oksidatif stres düzeylerini belirlemek için kullanıldı. indeks (OSI). CP verilen grupta lökosit, trombosit ve kemik iliği çekirdekli hücre sayılarında azalma görülürken, bu gruptaki ratların MDA ve TOC düzeylerinde GSH ve TAC düzeylerinde düşme dışında artış görüldü. Tersine, CP ile indüklenen oksidatif stres ve miyelosupresyon, kombine SV Gruplarında (CP+100 ve 200 mg kg -1 SV Gruplarında) tersine döndü, ancak ikinci gruptaki tersine dönüş daha önemliydi. Deneysel sonuçlarımız, SV'nin CP ile ilişkili periferik kan ve kemik iliği toksisiteleri üzerinde antioksidan ve sitoprotektif etkiler gösterebileceğini göstermiştir.

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Molecular docking analyses of Escin as regards cyclophosphamide-induced cardiotoxicity: In vivo and in Silico studies

Cited by 25 publications

References 24 publications

Prevention effect of total ginsenosides and ginseng extract from Panax ginseng on cyclophosphamide‐induced immunosuppression in mice

Prevention effect of total ginsenosides and ginseng extract from Panax ginseng on cyclophosphamide‐induced immunosuppression in mice

Molecular docking analysis and anti-apoptotic activity of Hypericum triquetrifolium Turra (St. John's-wort) in Cyclophosphamide-induced lung injury

Siklofosfamid Kaynaklı Toksisite Üzerine Salvia virgata Jacq'ın Hematoprotektif ve Antioksidan Etkilerinin Araştırılması

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