Molecular Docking of Multidrug Resistant  Klebsiella pneumoniae from River Water of  Klang Valley, Malaysia

Suryadevara, Nagaraja; Kwan, Yoke Ing; Shanmugam, Gnanendra; Ponmurugan, P.; Ganapathy, Balavinayagamani; Subramonie, Sridevi; Velaga, Venkatasathya Sai Appala Raju

doi:10.22207/jpam.13.4.26

Cited by 4 publications

(2 citation statements)

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“…In Malaysia, ESBL colonization is often reported in the clinical setting [ 25 – 28 ], mainly in studies of a large regional cohort [ 29 – 31 ], with some published articles on farm animals [ 32 ], foods [ 33 ], and the environment [ 6 , 34 ]. A comparison between ESBL-producing Klebsiella pneumoniae isolated from a swine and a clinical patient was recently reported [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Pan-genome and resistome analysis of extended-spectrum ß-lactamase-producing Escherichia coli: A multi-setting epidemiological surveillance study from Malaysia

et al. 2022

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Objectives This study profiled the prevalence of extended-spectrum ß-lactamase-producing Escherichia coli (ESBL-EC) in the community and compared their resistome and genomic profiles with isolates from clinical patients through whole-genome sequencing. Methods Fecal samples from 233 community dwellers from Segamat, a town in southern Malaysia, were obtained between May through August 2018. Putative ESBL strains were screened and tested using antibiotic susceptibility tests. Additionally, eight clinical ESBL-EC were obtained from a hospital in the same district between June through October 2020. Whole-genome sequencing was then conducted on selected ESBL-EC from both settings (n = 40) for pan-genome comparison, cluster analysis, and resistome profiling. Results A mean ESBL-EC carriage rate of 17.82% (95% CI: 10.48%– 24.11%) was observed in the community and was consistent across demographic factors. Whole-genome sequences of the ESBL-EC (n = 40) enabled the detection of multiple plasmid replicon groups (n = 28), resistance genes (n = 34) and virulence factors (n = 335), with no significant difference in the number of genes carried between the community and clinical isolates (plasmid replicon groups, p = 0.13; resistance genes, p = 0.47; virulence factors, p = 0.94). Virulence gene marker analysis detected the presence of extraintestinal pathogenic E. coli (ExPEC), uropathogenic E. coli (UPEC), and enteroaggregative E. coli (EAEC) in both the community and clinical isolates. Multiple blaCTX-M variants were observed, dominated by blaCTX-M-27 (n = 12), blaCTX-M-65 (n = 10), and blaCTX-M-15 (n = 9). The clinical and community isolates did not cluster together based on the pan-genome comparison, suggesting isolates from the two settings were clonally unrelated. However, cluster analysis based on carried plasmids, resistance genes and phenotypic susceptibility profiles identified four distinct clusters, with similar patterns between the community and clinical isolates. Conclusion ESBL-EC from the clinical and community settings shared similar resistome profiles, suggesting the frequent exchange of genetic materials through horizontal gene transfer.

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Section: Introductionmentioning

confidence: 99%

Pan-genome and resistome analysis of extended-spectrum ß-lactamase-producing Escherichia coli: A multi-setting epidemiological surveillance study from Malaysia

et al. 2022

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“…K21 is a predominant serotype [ 81 , 82 , 83 ], while K24 was reported to be associated with sequence type-15 (ST15) [ 84 , 85 , 86 ] and frequently reported from other carbapenemase-producing Klebsiella strains [ 8 ]. K33 is not a markedly significant capsular serotype in terms of virulence or epidemiological prevalence, however, it was reported and/or examined in a set of studies in Europe and Asia [ 87 , 88 ], most of them investigating multidrug-resistant (MDR) K. pneumoniae isolates [ 89 , 90 , 91 , 92 , 93 ]. K33 was reported prevalent in urinary tract infections (UTIs) [ 94 ], isolated as nosocomial pathogen from urine [ 90 ], blood [ 88 ], from ventilators and bedrails of an ICU [ 95 ], and also reported as the most common serotype along with K69 in surface water isolates [ 96 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Lytic Bacteriophage and Demonstration of Its Combined Lytic Effect with a K2 Depolymerase on the Hypervirulent Klebsiella pneumoniae Strain 52145

2023

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Klebsiella pneumoniae is a nosocomial pathogen. Among its virulence factors is the capsule with a prominent role in defense and biofilm formation. Bacteriophages (phages) can evoke the lysis of bacterial cells. Due to the mode of action of their polysaccharide depolymerase enzymes, phages are typically specific for one bacterial strain and its capsule type. In this study, we characterized a bacteriophage against the capsule-defective mutant of the nosocomial K. pneumoniae 52145 strain, which lacks K2 capsule. The phage showed a relatively narrow host range but evoked lysis on a few strains with capsular serotypes K33, K21, and K24. Phylogenetic analysis showed that the newly isolated Klebsiella phage 731 belongs to the Webervirus genus in the Drexlerviridae family; it has a 31.084 MDa double-stranded, linear DNA with a length of 50,306 base pairs and a G + C content of 50.9%. Out of the 79 open reading frames (ORFs), we performed the identification of orf22, coding for a trimeric tail fiber protein with putative capsule depolymerase activity, along with the mapping of other putative depolymerases of phage 731 and homologous phages. Efficacy of a previously described recombinant K2 depolymerase (B1dep) was tested by co-spotting phage 731 on K. pneumoniae strains, and it was demonstrated that the B1dep-phage 731 combination allows the lysis of the wild type 52145 strain, originally resistant to the phage 731. With phage 731, we showed that B1dep is a promising candidate for use as a possible antimicrobial agent, as it renders the virulent strain defenseless against other phages. Phage 731 alone is also important due to its efficacy on K. pneumoniae strains possessing epidemiologically important serotypes.

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Antimicrobial resistance in southeast Asian water environments: A systematic review of current evidence and future research directions

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et al. 2023

Science of The Total Environment

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Molecular Docking of Multidrug Resistant Klebsiella pneumoniae from River Water of Klang Valley, Malaysia

Cited by 4 publications

References 24 publications

Pan-genome and resistome analysis of extended-spectrum ß-lactamase-producing Escherichia coli: A multi-setting epidemiological surveillance study from Malaysia

Pan-genome and resistome analysis of extended-spectrum ß-lactamase-producing Escherichia coli: A multi-setting epidemiological surveillance study from Malaysia

Characterization of a Lytic Bacteriophage and Demonstration of Its Combined Lytic Effect with a K2 Depolymerase on the Hypervirulent Klebsiella pneumoniae Strain 52145

Antimicrobial resistance in southeast Asian water environments: A systematic review of current evidence and future research directions

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