Molecular dynamics of estrogen receptors

Okat, Zehra

doi:10.14744/ejmo.2018.76476

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…Estrogens' biological and physiological functions are executed by binding to the cognate receptors known as estrogen receptors ( ERs ). The two primary receptors in poultry are estrogen receptor 1 ( ESR1/ ERα/ ER1 ) and estrogen receptor 2 ( ESR2/ERβ / ER2 ), which belong to the nuclear receptor superfamily ( Murphy et al, 1998 ; Okat, 2018 ; Chen et al, 2019 ). The ERs act as transcription factors to initiate gene transcription through estrogen response elements ( EREs ) in the target tissues and interact with other transcription factors ( Hall and McDonnel, 1999 ).…”

Section: Introductionmentioning

confidence: 99%

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Asiamah

Liu

et al. 2022

Poultry Science

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Our previous study on the ovarian transcriptomic analysis in Leizhou black duck revealed that the ESR2 gene was involved in hormone regulation in reproduction and the estrogen signaling pathway related to reproductive performance was enriched. This suggested that ESR2 may have a functional role in the reproductive performance of the Leizhou black duck. Thus, this study aimed at evaluating the polymorphism of the ESR2 gene and its association with egg-laying traits and the distribution pattern of ESR2 mRNA in laying and non-laying Leizhou black ducks. In this study, genomic DNA was extracted from blood samples of 101 Leizhou black ducks to identify single nucleotide polymorphisms ( SNPs ) of the ESR2 gene to elucidate molecular markers highly associated with egg-laying traits. Four each of laying and non-laying Leizhou black ducks were selected to collect different tissues to analyze the ESR2 gene expression. A total of 23 SNPs were identified and association analysis of the single SNP sites showed that SNPs g.56805646 T>C and exon 3-20G>A were significantly ( P < 0.05) associated with egg weight. Ducks with CT and AG genotypes had significantly higher (P < 0.05) egg weights than their respective other genotypes. Haplotype association analysis of g.56805646 T>C and exon 3-20G>A showed that the haplotypes were significantly associated with egg weight. Higher egg weight was seen in individuals with H3H4 haplotypes. In the hypothalamus-pituitary-gonadal ( HPG ) axis, the results of qRT/PCR showed that ESR2 mRNA was significantly ( P < 0.05) expressed in the ovaries of both duck groups than in the hypothalamus and pituitary. In the oviduct, ESR2 was significantly ( P < 0.05) higher in the infundibulum and magnum of laying and non-laying ducks respectively. This study provides a molecular marker for selecting Leizhou black ducks for egg production. In addition, it offers theoretical knowledge for studying the related biological functions of the ESR2 gene at the cellular level.

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Section: Introductionmentioning

confidence: 99%

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Asiamah

Liu

et al. 2022

Poultry Science

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“…To elucidate the possible relationship between the ESR2 gene with egg-laying traits, we designed four (4) different primers and examined SNPs in both coding and non-coding regions. Each of the four (primers 1,2,3, and 4) were found to have eight (8), nine (9), four (4), and two (2) SNP sites respectively, a total of 23 SNP sites. Out of the 23 SNP sites, only two (2) of them; exon 2-160C>T (primer 5) and exon 3-20G>A (primer 6) were found in the coding region.…”

Section: Genetic Polymorphism Of Esr2 Genementioning

confidence: 99%

Polymorphism Analysis and Expression Profile of the Estrogen Receptor 2 Gene in Leizhou Black Duck

Asiamah

Liu

et al. 2021

Preprint

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BackgroundOur previous study on the ovarian transcriptomic analysis in Leizhou black duck revealed that the ESR2 gene was involved in hormone regulation in reproduction and the estrogen signaling pathway related to reproductive performance was enriched. This suggested that ESR2 may have a functional role in the reproductive performance of the Leizhou black duck. Thus, this study aimed at evaluating the polymorphism of the ESR2 gene and its association with egg-laying traits and the distribution pattern of ESR2 mRNA in laying and non-laying Leizhou black ducks. MethodIn this study, genomic DNA was extracted from blood samples of 101 Leizhou black ducks to identify single nucleotide polymorphisms (SNPs) of the ESR2 gene to elucidate molecular markers highly associated with egg-laying traits. Four (4) each of laying and non-laying Leizhou black ducks were selected to collect different tissues to analyze the ESR2 gene expression. ResultsA total of 23 SNPs were identified and association analysis of the single SNP sites showed that SNPs g.56805646 T>C and exon 3-20G>A were significantly (P < 0.05) associated with egg weight. Ducks with CT and AG genotypes had significantly higher (P < 0.05) egg weights than their respective other genotypes. Haplotype association analysis of g.56805646 T>C and exon 320G>A showed that the haplotypes were significantly associated with egg weight where higher egg weight was seen in individuals with H3H4 haplotypes. In the hypothalamus-pituitary-gonadal (HPG) axis, the results of qRT/PCR showed that ESR2 mRNA was significantly (P < 0.05) expressed in the ovaries of both duck groups than in the hypothalamus and pituitary. In the oviduct, ESR2 was significantly (P < 0.05) higher in the infundibulum and magnum of laying and nonlaying ducks respectively. ConclusionThis study provides molecular marker for selecting Leizhou black ducks for egg production and provides theoretical knowledge for the study of the related biological functions of the ESR2 gene at the cellular level.

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“…ERK1 and ERK2 of the MAPK family modulate the expression regulation by phosphorylating ER at serine 118 position within AF-1 domain. This increases the likelihood of ER for ligand and activates ER directly -independent of ligand (Okat, 2018).Besides, serine 167 is also phosphorylated in AF-1 domain of ER through the action of ribosomal S6 kinase (RS6) which is itself activated by ERK1 and ERK2. This means that increase in the expression of ERK1/2 can possibly confer resistance to anti-estrogen regimen (Shou et al, 2004).…”

Section: Growth Factor Signalingmentioning

confidence: 99%

Tamoxifen resistance mechanisms in breast cancer treatment

Tayyeb¹

2020

World J. Adv. Res. Rev.

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Therapies targeting estrogen receptor (ER) are being widely used to treat ER+ breast cancer patients. Despite early detection and improved survival outcomes, tamoxifen resistance, either intrinsic or acquired- is a major obstacle in effective disease management. Current review will summarize different molecular mechanisms of tamoxifen resistance both intrinsic and acquired in breast cancer treatment. This review not only provides basis to understand the nature of tamoxifen drug resistance but also suggests the mechanisms for its control leading to improved therapeutic interventions.

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Molecular dynamics of estrogen receptors

Cited by 5 publications

References 34 publications

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Polymorphism analysis and expression profile of the estrogen receptor 2 gene in Leizhou black duck

Polymorphism Analysis and Expression Profile of the Estrogen Receptor 2 Gene in Leizhou Black Duck

Tamoxifen resistance mechanisms in breast cancer treatment

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