Molecular ecology of Triatoma dimidiata in southern Belize reveals risk for human infection and the local differentiation of Trypanosoma cruzi parasites

Polonio, Roy; López-Domínguez, Jaime; Herrera, Claudia; Dumonteil, Eric

doi:10.1016/j.ijid.2021.05.083

Cited by 11 publications

(6 citation statements)

References 51 publications

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“…Amplicon deep sequencing fills the need for a powerful method that detects not only the most recent/abundant host DNA, but also older and partially degraded bloodmeals as well. This method is becoming increasingly used in the detection of bloodmeals from triatomines yielding multiple host information from individual bugs allowing comparison of triatomines from different habitats, understanding their behaviors and controlling the spread of Chagas disease 13 – 15 , 52 .…”

Section: Discussionmentioning

confidence: 99%

Characterization of triatomine bloodmeal sources using direct Sanger sequencing and amplicon deep sequencing methods

Balasubramanian

Curtis‐Robles

Chirra

et al. 2022

Sci Rep

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Knowledge of host associations of blood-feeding vectors may afford insights into managing disease systems and protecting public health. However, the ability of methods to distinguish bloodmeal sources varies widely. We used two methods—Sanger sequencing and amplicon deep sequencing—to target a 228 bp region of the vertebrate Cytochrome b gene and determine hosts fed upon by triatomines (n = 115) collected primarily in Texas, USA. Direct Sanger sequencing of PCR amplicons was successful for 36 samples (31%). Sanger sequencing revealed 15 distinct host species, which included humans, domestic animals (Canis lupus familiaris, Ovis aries, Gallus gallus, Bos taurus, Felis catus, and Capra hircus), wildlife (Rattus rattus, Incilius nebulifer, Sciurus carolinensis, Sciurus niger, and Odocoileus virginianus), and captive animals (Panthera tigris, Colobus spp., and Chelonoidis carbonaria). Samples sequenced by the Sanger method were also subjected to Illumina MiSeq amplicon deep sequencing. The amplicon deep sequencing results (average of 302,080 usable reads per sample) replicated the host community revealed using Sanger sequencing, and detected additional hosts in five triatomines (13.9%), including two additional blood sources (Procyon lotor and Bassariscus astutus). Up to four bloodmeal sources were detected in a single triatomine (I. nebulifer, Homo sapiens, C. lupus familiaris, and S. carolinensis). Enhanced understanding of vector-host-parasite networks may allow for integrated vector management programs focusing on highly-utilized and highly-infected host species.

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Section: Discussionmentioning

confidence: 99%

Characterization of triatomine bloodmeal sources using direct Sanger sequencing and amplicon deep sequencing methods

Balasubramanian

Curtis‐Robles

Chirra

et al. 2022

Sci Rep

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“…After mapping using all the DTUs as reference, a total of 89,746 sequences of good quality mapped only to the TcIV DTU. The phylogenetic analysis indicated four unique TcIV min-exon sequence haplotypes, which were most closely related to the TcIV T. cruzi sequences identified from T. dimidiata vectors previously sequenced from southern Belize 13 as well as the acute Chagas disease pediatric case-patient 18 represented in this study. Yet they were distinct from the T. cruzi TcIV reference, CanIII, from Brazil (Fig.…”

Section: Resultsmentioning

confidence: 57%

“…and T. huehuetenanguensis (formerly published as clade III T. dimidiata s.l.) were thought to be predominately sylvatic in nature, but able to seasonally infest houses, and hybrids have also been identified 12 , 13 , 15 . While our findings from this small sampling are not robust enough to draw significant conclusions about vector ecology of this potential new species, we made some observations of note.…”

Section: Discussionmentioning

confidence: 99%

Novel species of Triatoma (Hemiptera: Reduviidae) identified in a case of vectorial transmission of Chagas disease in northern Belize

Gunter,

Nelson,

Kneubehl

et al. 2024

Sci Rep

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Chagas disease is a leading cause of non-ischemic cardiomyopathy in endemic regions of Central and South America. In Belize, Triatoma dimidiata sensu lato has been identified as the predominate taxon but vectorial transmission of Chagas disease is considered to be rare in the country. We recently identified an acute case of vector-borne Chagas disease in the northern region of Belize. Here we present a subsequent investigation of triatomines collected around the case-patient’s home. We identified yet undescribed species, closely related to Triatoma huehuetenanguensis vector by molecular systematics methods occurring in the peridomestic environment. The identification of a T. cruzi-positive, novel species of Triatoma in Belize indicates an increased risk of transmission to humans in the region and warrants expanded surveillance and further investigation.

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“…A general trend in field-caught bugs is that nymphs have a highly diverse microbiota, which often differs from that of adult bugs, suggesting different metabolic functions and interactions. This is the case for Triatoma protracta [17], Triatoma reticularia [17], Triatoma rubida [17], Triatoma dimidiata [18,19], and Rhodnius prolixus [20]. On the other hand, in Triatoma sordida the adult microbiota is more diverse compared to nymphs, with unique bacteria being incorporated from the 4 th instar stage onwards [21].…”

Section: Introductionmentioning

confidence: 98%

Metabolomics of developmental changes in Triatoma sanguisuga gut microbiota

2023

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Triatoma sanguisuga is one of the major vectors of Trypanosoma cruzi in the southeastern US, where it sustains a robust zoonotic parasite transmission cycle and occasional human infections. A better understanding of triatomine development may allow for alternative approaches to insecticide-based vector control. Indeed, the role of the gut microbiota and bacterial endosymbionts in triatomine development and in their vectorial capacity is emerging. We investigated here the differences in microbiota among nymph and adult T. sanguisuga, to shed light on the metabolomic interactions occurring during development. Microbiota composition was assessed by 16s gene amplification and deep sequencing from field-caught adult bugs and their laboratory-raised progeny. Significant differences in microbiota bacterial diversity and composition were observed between nymphs and adults. Laboratory-raised nymphs showed a higher taxonomic diversity, and at least seven families predominated. On the other hand, field-caught adults had a lower bacterial diversity and four families comprised most of the microbiota. These differences in compositions were associated with differences in predicted metabolism, with laboratory-raised nymphs microbiota metabolizing a limited diversity of carbon sources, with potential for resource competition between bacterial families, and the production of lactic acid as a predominant fermentation product. On the other hand, field-caught adult microbiota was predicted to metabolize a broader diversity of carbon sources, with complementarity rather than competition among taxa, and produced a diverse range of products in a more balanced manner. The restricted functionality of laboratory-raised nymph microbiota may be associated with their poor development in captivity, and further understanding of the metabolic interactions at play may lead to alternative vector control strategies targeting triatomine microbiota.

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Molecular ecology of Triatoma dimidiata in southern Belize reveals risk for human infection and the local differentiation of Trypanosoma cruzi parasites

Cited by 11 publications

References 51 publications

Characterization of triatomine bloodmeal sources using direct Sanger sequencing and amplicon deep sequencing methods

Characterization of triatomine bloodmeal sources using direct Sanger sequencing and amplicon deep sequencing methods

Novel species of Triatoma (Hemiptera: Reduviidae) identified in a case of vectorial transmission of Chagas disease in northern Belize

Metabolomics of developmental changes in Triatoma sanguisuga gut microbiota

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