Molecular effects of photon irradiation and subsequent aftercare treatment with dexpanthenol‐containing ointment or liquid in 3D models of human skin and non‐keratinized oral mucosa

Huth, Sebastian; Marquardt, Yvonne; Huth, Laura; Schmitt, Laurenz; Prescher, Kirsten; Winterhalder, Philipp; Steiner, Timm; Hölzle, Frank; Eble, Michael J.; Baron, Jens Malte

doi:10.1111/exd.14266

Cited by 5 publications

(5 citation statements)

References 24 publications

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“…Similar results were found with a reduction of postoperative pain and signs of inflammation in a randomized and placebo-controlled clinical trial [22]. Another in vitro study showed that a dexpanthenol-containing ointment had a favorable impact on structures critical to antimicrobial barrier function and had anti-inflammatory effects after photon irradiation [23].…”

Section: Introductionsupporting

confidence: 69%

Effect of Dentifrice Ingredients on Volume and Vitality of a Simulated Periodontal Multispecies Biofilm

Karacic,

Ruf,

Herzog

et al. 2024

Dentistry Journal

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The aim of this in vitro study was to investigate the effect of different toothpaste ingredients on biofilm volume and vitality in an established non-contact biofilm removal model. A multi-species biofilm comprising Porphyromonas gingivalis, Streptococcus sanguinis, and Fusobacterium nucleatum was grown on protein-coated titanium disks. Six disks per group were exposed to 4 seconds non-contact brushing using a sonic toothbrush. Four groups assessed slurries containing different ingredients, i.e., dexpanthenol (DP), peppermint oil (PO), cocamidopropyl betaine (CB), and sodium hydroxide (NaOH), one positive control group with the slurry of a toothpaste (POS), and a negative control group with physiological saline (NEG). Biofilm volume and vitality were measured using live-dead staining and confocal laser scanning microscopy. Statistical analysis comprised descriptive statistics and inter-group differences. In the test groups, lowest vitality and volume were found for CB (50.2 ± 11.9%) and PO (3.6 × 105 ± 1.8 × 105 µm3), respectively. Significant differences regarding biofilm vitality were found comparing CB and PO (p = 0.033), CB and NEG (p = 0.014), NaOH and NEG (p = 0.033), and POS and NEG (p = 0.037). However, no significant inter-group differences for biofilm volume were observed. These findings suggest that CB as a toothpaste ingredient had a considerable impact on biofilm vitality even in a non-contact brushing setting, while no considerable impact on biofilm volume was found.

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Section: Introductionsupporting

confidence: 69%

Effect of Dentifrice Ingredients on Volume and Vitality of a Simulated Periodontal Multispecies Biofilm

Karacic,

Ruf,

Herzog

et al. 2024

Dentistry Journal

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“…Thus, it is conceivable that macrophage activation is greater with PLLA‐SCA than CaHA‐CMC and may explain differences in mechanism of action, with PLLA‐SCA having an immune‐mediated regenerative effect driven primarily by M2 macrophage polarization and CaHA‐CMC having a mechanotransductive effect independent of robust inflammation 4,33 . This hypothesis is supported by a recent gene expression study that showed PLLA‐SCA's mechanism of action largely functioned through the upregulation of inflammasomes IL1‐α, IL1‐β, and CXCL6, which contributed to dermal thickening and neocollagenesis, 16 a study showing macrophage‐dependent neocollagenesis with PLLA‐SCA 11 , and studies showing contact‐dependent neocollagenesis with CaHA‐CMC 4 . The body's ability to recognize size discrepancies in foreign objects underscores the importance of understanding and optimizing particle morphology to minimize adverse immune reactions and enhance therapeutic efficacy.…”

Section: Discussionmentioning

confidence: 94%

“…showed that PLLA particles were encapsulated rather by the less organized collagen type III than type I 14 . Data suggests that the mechanism of action of PLLA relies on the involvement of the immune system for neocollagenesis, a distinctly different mechanism of action compared to CaHA‐CMC 11,15,16 . Previous studies indicate the particles that compose PLLA are heterogeneous and resemble flakes, rather than smooth microspheres 17 .…”

Section: Introductionmentioning

confidence: 99%

“…This irregular morphology may compromise the direct filling effect of PLLA‐SCA, while simultaneously eliciting a more pronounced inflammatory response due to the presence of small fragmented particles and sharp particle edges 14 . Moreover, recent studies have highlighted PLLA‐SCA's propensity to elicit increased inflammatory cell response and activation of pro‐inflammatory cytokines, particularly M1 and M2 macrophages 16,18 . Confocal imaging has provided insights into the formation of macrophage and giant cell capsules at the surface of PLLA‐SCA particles, further underscoring its immunostimulatory effects 19 …”

Section: Introductionmentioning

confidence: 99%

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A morphological analysis of calcium hydroxylapatite and poly‐l‐lactic acid biostimulator particles

McCarthy,

Hartmann,

Durkin

et al. 2024

Skin Research and Technology

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Injectable fillers, pivotal in aesthetic medicine, have evolved significantly with recent trends favoring biostimulators like calcium hydroxylapatite (CaHA‐CMC; Radiesse, Merz Aesthetics, Raleigh, NC) and poly‐l‐lactic acid (PLLA; Sculptra Aesthetics, Galderma, Dallas, TX). This study aims to compare the particle morphology of these two injectables and examine its potential clinical implications. Utilizing advanced light and scanning electron microscopy techniques, the physical characteristics of CaHA‐CMC and PLLA particles were analyzed, including shape, size, circularity, roundness, aspect ratio, and quantity of phagocytosable particles. The findings reveal several morphological contrasts: CaHA‐CMC particles exhibited a smooth, homogenous, spherical morphology with diameters predominantly ranging between 20 and 45 µm, while PLLA particles varied considerably in shape and size, appearing as micro flakes ranging from 2 to 150 µm in major axis length. The circularity and roundness of CaHA‐CMC particles were significantly higher compared to PLLA, indicating a more uniform shape. Aspect ratio analysis further underscored these differences, with CaHA‐CMC particles showing a closer resemblance to circles, unlike the more oblong PLLA particles. Quantification of the phagocytosable content of both injectables revealed a higher percentage of phagocytosable particles in PLLA. These morphological distinctions may influence the tissue response to each treatment. CaHA‐CMC's uniform, spherical particles may result in reduced inflammatory cell recruitment, whereas PLLA's heterogeneous particle morphology may evoke a more pronounced inflammatory response.

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“…Schmutz et al reported non-inferiority in maintaining TEWL scores in acute radiation dermatitis (0.1% methylprednisolone cream vs. 0.5% dexpanthenol cream) [ 75 ]. As shown in full-thickness 3D skin models representing acute radiodermatitis and mucositis, skin impairment seven days after radiotherapy demonstrated a completely restored epidermal part after treatment with dexpanthenol-containing ointment or liquid [ 76 ].…”

Section: How Dexpanthenol Can Help In Admentioning

confidence: 99%

Use of Dexpanthenol for Atopic Dermatitis—Benefits and Recommendations Based on Current Evidence

Cho

Kim

Woo

et al. 2022

JCM

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Background: Atopic dermatitis (AD) is an inflammatory skin disease of multiple phenotypes and endotypes, and is highly prevalent in children. Many people of all ages, including active adolescents, pregnant women, and the elderly, suffer from AD, experiencing chronicity, flares, and unexpected relapse. Dexpanthenol has multiple pharmacological effects and has been employed to treat various skin disorders such as AD. We aimed to summarize the up-to-date evidence relating to dexpanthenol and to provide a consensus on how to use dexpanthenol effectively for the treatment of AD. Methods: The evidence to date on the application and efficacy of dexpanthenol in AD was reviewed. The literature search focused on dexpanthenol use and the improvement of skin barrier function, the prevention of acute flares, and its topical corticosteroid (TCS) sparing effects. Evidence and recommendations for special groups such as pregnant women, and the effects of dexpanthenol and emollient plus in maintenance therapy, were also summarized. Results: Dexpanthenol is effective and well-tolerated for the treatment of AD. Dexpanthenol improves skin barrier function, reduces acute and frequent flares, has a significant TCS sparing effect, and enhances wound healing for skin lesions. Conclusion: This review article provides helpful advice for clinicians and patients on the proper maintenance treatment of AD. Dexpanthenol, as an active ingredient in ointments or emollients, is suitable for the treatment and maintenance of AD. This paper will guide dermatologists and clinicians to consider dexpanthenol as a treatment option for mild to moderate AD.

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Molecular effects of photon irradiation and subsequent aftercare treatment with dexpanthenol‐containing ointment or liquid in 3D models of human skin and non‐keratinized oral mucosa

Cited by 5 publications

References 24 publications

Effect of Dentifrice Ingredients on Volume and Vitality of a Simulated Periodontal Multispecies Biofilm

Effect of Dentifrice Ingredients on Volume and Vitality of a Simulated Periodontal Multispecies Biofilm

A morphological analysis of calcium hydroxylapatite and poly‐l‐lactic acid biostimulator particles

Use of Dexpanthenol for Atopic Dermatitis—Benefits and Recommendations Based on Current Evidence

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