Molecular Engineering of Cell and Tissue Surfaces with Polymer Thin Films

Wilson, John T.; Chaikof, Elliot L.

doi:10.1016/b978-1-4557-3146-6.00013-1

Cited by 3 publications

(1 citation statement)

References 129 publications

(187 reference statements)

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“…Cells immersed in the polymeric solutions produced a polycationic coating that was speckled rather than a complete capsule (Figure 1), as a result of electrostatic attraction of PLL to the cell membrane, with the positively charged polymer attracted by the negatively charged membrane. 25 Figure 1. Illustration of temporary cellular speckled coating using poly(L-lysine) and further uptake of the biodegradable polycation.…”

Section: Resultsmentioning

confidence: 99%

Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer

Ribeiro

Pal

Jamieson³

et al. 2017

ACS Appl. Mater. Interfaces

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Temporary single-cell coating is a useful tool for cell processing, allowing manipulation of cells to prevent cell attachment and agglomeration, before re-establishing normal cell function. In this work, a speckled coating method using a known polycation [poly(l-lysine), PLL] is described to induce cell surface electrostatic charges on three different cell types, namely, two bone cancer cell lines and fibroblasts. The morphology of the PLL speckled coating on the cell surface, internalization and metabolization of the polymer, and prevention of cellular aggregations are reported. Polymer concentration was found to be the key parameter controlling both capsule morphology and cell health. This approach allows a temporary cell coating over the course of 1–2 h, with cells exhibiting phenotypically normal behavior after ingesting and metabolizing the polymer. The process offers a fast and efficient alternative to aid single-cell manipulation for bioprocessing applications. Preliminary work on the application of PLL speckled cell coating in enabling reliable bioprinting is also presented.

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Section: Resultsmentioning

confidence: 99%

Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer

Ribeiro

Pal

Jamieson³

et al. 2017

ACS Appl. Mater. Interfaces

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Engineering live cell surfaces with functional polymers via cytocompatible controlled radical polymerization

et al. 2017

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The capability to graft synthetic polymers onto the surfaces of live cells offers the potential to manipulate and control their phenotype and underlying cellular processes. Conventional grafting-to strategies for conjugating preformed polymers to cell surfaces are limited by low polymer grafting efficiency. Here we report an alternative grafting-from strategy for directly engineering the surfaces of live yeast and mammalian cells through cell surface-initiated controlled radical polymerization. By developing cytocompatible PET-RAFT (photoinduced electron transfer-reversible addition-fragmentation chain-transfer polymerization), synthetic polymers with narrow polydispersity (M/M < 1.3) could be obtained at room temperature in 5 minutes. This polymerization strategy enables chain growth to be initiated directly from chain-transfer agents anchored on the surface of live cells using either covalent attachment or non-covalent insertion, while maintaining high cell viability. Compared with conventional grafting-to approaches, these methods significantly improve the efficiency of grafting polymer chains and enable the active manipulation of cellular phenotypes.

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Oncotically Driven Control over Glycocalyx Dimension for Cell Surface Engineering and Protein Binding in the Longitudinal Direction

Siren

Chapanian

Constantinescu

et al. 2018

Sci Rep

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Here we present a simple technique for re-directing reactions on the cell surface to the outermost region of the glycocalyx. Macromolecular crowding with inert polymers was utilized to reversibly alter the accessibility of glycocalyx proteoglycans toward cell-surface reactive probes allowing for reactivity control in the longitudinal direction (‘z’-direction) on the glycocalyx. Studies in HUVECs demonstrated an oncotically driven collapse of the glycocalyx brush structure in the presence of crowders as the mechanism responsible for re-directing reactivity. This phenomenon is consistent across a variety of macromolecular agents including polymers, protein markers and antibodies which all displayed enhanced binding to the outermost surface of multiple cell types. We then demonstrated the biological significance of the technique by increasing the camouflage of red blood cell surface antigens via a crowding-enhanced attachment of voluminous polymers to the exterior of the glycocalyx. The accessibility to Rhesus D (RhD) and CD47 proteins on the cell surface was significantly decreased in crowding-assisted polymer grafting in comparison to non-crowded conditions. This strategy is expected to generate new tools for controlled glycocalyx engineering, probing the glycocalyx structure and function, and improving the development of cell based therapies.

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Molecular Engineering of Cell and Tissue Surfaces with Polymer Thin Films

Cited by 3 publications

References 129 publications

Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer

Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer

Engineering live cell surfaces with functional polymers via cytocompatible controlled radical polymerization

Oncotically Driven Control over Glycocalyx Dimension for Cell Surface Engineering and Protein Binding in the Longitudinal Direction

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