Molecular Engineering Powered Dual-Readout Point-of-Care Testing for Sensitive Detection of Escherichia coli O157:H7

Dual-Readout Ultrasensitive Lateral Flow Immunosensing of Salmonella typhimurium in Dairy Products by Doping Engineering-Powered Nanoheterostructure with Enhanced Photothermal Performance

“…Foodborne gastrointestinal bacterial pathogens are one of the major foodborne pathogenic bacteria causing human diarrheal diseases, such as diarrhea, abdominal cramping, and vomiting. − Foodborne pathogens are widely polluted, spread swiftly, and do excellent harm, which has a great impact on human health and the economy and has turned out to be a premier public health problem in the world. , Among them, Salmonella typhimurium (S. typhimurium) is a particularly notorious intestinal pathogen, which is associated with the outbreak of numerous foodborne diseases. , The incidence rate of S.…”

Ferrous sulfate combined with ultrasound emulsified cinnamaldehyde nanoemulsion to cause ferroptosis in Escherichia coli O157:H7

“…Escherichia coli O157:H7 is a Gram-negative foodborne pathogen that causes a variety of human disease including hemorrhagic colitis, thrombotic thrombocytopenic purpura, hemolytic uremic syndrome, and mild diarrhea in humans. The bacteria were identified as foodborne pathogen in 1982, which has the characteristics of very low infectious dose (approximately 10–100 CFU/mL or g) and inherent acid tolerance [1] , [2] . E. coli O157:H7 usually contaminates drinking water and various foods including milk, meat, fruit, vegetables, and so on.…”

“Cave Effect” Induces Self-Assembled Bimetallic Hollow Structure for Three-in-One Lateral Flow Immunoassay

“…The multimode signal output was mainly obtained by the excellent characteristics of nanomaterials themselves, as an important part of LFIA, nanomaterials directly affected the sensitivity of detection. 5,6,16 Therefore, it was imperative to develop a multifunctional nanomaterial for the LFIA to improve the sensitivity.…”

“…However, single-mode conventional gold nanoparticle detection was limited due to insufficient colorimetric brightness and low sensitivity . In contrast, multimodal detection involved two or more modes that were correlated with each other and amplified immune signals to obtain more sensitive and reliable experimental results than single-mode detection. − It not only had the advantages of traditional colorimetric mode but also introduced quantitative detection technology that can be substituted and complementary. The multimode LFIA detection can output signals of different functions through nanomaterials (quantum dots, nanomases, metal–organic frameworks, , etc.)…”

“…and specific antibodies (monoclonal antibodies, polyclonal antibodies, nanoantibodies), which mainly include colorimetric signal, fluorescence signal, photothermal signal, catalytic signal, electrochemical signal and surface-enhanced Raman scattering (SERS) signal, providing more sensitive quantitative analysis results. The multimode signal output was mainly obtained by the excellent characteristics of nanomaterials themselves, as an important part of LFIA, nanomaterials directly affected the sensitivity of detection. ,, Therefore, it was imperative to develop a multifunctional nanomaterial for the LFIA to improve the sensitivity.…”