Molecular epidemiological analysis of SARS-CoV-2 genovariants in Moscow and Moscow region

Ozhmegova, Ekaterina; Savochkina, Tatyana E.; Прилипов, А. Г.; Tikhomirov, E. .E.; Larichev, Victor; Сайфуллин, М. А.; Гребенникова, Т. В.

doi:10.36233/0507-4088-146

Problems of Virology

2022

DOI: 10.36233/0507-4088-146

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Molecular epidemiological analysis of SARS-CoV-2 genovariants in Moscow and Moscow region

Ekaterina Ozhmegova¹,

Tatyana E. Savochkina²,

А. Г. Прилипов³

et al.

Abstract: Introduction. SARS-CoV-2, a severe acute respiratory illness virus that emerged in China in late 2019, continues to spread rapidly around the world, accumulating mutations and thus causing serious concern. Five virus variants of concern are currently known: Alpha (lineage B.1.1.7), Beta (lineage B.1.351), Gamma (lineage P.1), Delta (lineage B.1.617.2), and Omicron (lineage B.1.1.529). In this study, we conducted a molecular epidemiological analysis of the most prevalent genovariants in Moscow and the region.&#… Show more

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Cited by 2 publications

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COVID-19 Epidemic in the Kaliningrad Region: Incidence and Infection Control Measures

Molchanova¹,

Babura²,

Zaguzov³

et al. 2023

PH&LE

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Introduction: Combating the epidemic of the novel coronavirus disease required decision-making at the state level and joining efforts of medical workers of all specialties. The experience of the Kaliningrad Region deserves special attention since this region is located in Central Europe and the first COVID-19 cases were imported here in March 2020. Objective: To assess COVID-19 epidemic manifestations and effectiveness of preventive measures at the regional level. Materials and methods: We performed a retrospective epidemiological analysis of COVID-19 incidence in the Kaliningrad Region from March 2020 to December 2022. All registered cases were confirmed by PCR or immunochromatography assay. The study included 193,259 cases, 1,879 fatal cases, 1,168 samples with the established SARS-CoV-2 genetic variant, and information on 693,627 people vaccinated against COVID-19. The data were analyzed in Microsoft Excel and WinPepi (version 11.65). Results: In 2020, the COVID-19 incidence rate in the Kaliningrad Region was lower than that in the Russian Federation while in the years 2021 and 2022 the regional rates were, on the opposite, higher than the respective national ones (p < 0.05). All age groups of the population were involved in the outbreak. Periodic rises in the incidence were associated with the replacement of the Delta variant of SARS-CoV-2 with Omicron. We established high efficacy of vaccination against COVID-19: incidence and mortality rates among the unvaccinated were significantly higher than those among vaccinated individuals (p < 0.05). Conclusion: Organization of uniform infection control measures has enabled timely interdepartmental managerial decision-making to control the epidemic. Russian regions have accumulated unique experience in implementing a set of preventive and anti-epidemic measures to combat the epidemic of the novel infection.

show abstract

COVID-19 Epidemic in the Kaliningrad Region: Incidence and Infection Control Measures

Molchanova¹,

Babura²,

Zaguzov³

et al. 2023

PH&LE

View full text Add to dashboard Cite

show abstract

Development, production and characterization of SARS-CoV-2 virus-like particles (Coronaviridae: <i>Orthocoronavirinae: Betacoronavirus: Sarbecovirus</i>)

Latyshev,

Zaykova,

Eliseeva

et al. 2024

Problems of Virology

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Introduction. The COVID-19 pandemic caused by SARS-CoV-2 has created serious health problems worldwide. The most effective way to prevent the occurrence of new epidemic outbreaks is vaccination. One of the modern and effective approaches to vaccine development is the use of virus-like particles (VLPs). The aim of the study is to develop a technology for production of VLP based on recombinant SARS-CoV-2 proteins (E, M, N and S) in insect cells. Materials and methods. Synthetic genes encoding coronavirus proteins E, M, N and S were used. VLP with various surface proteins of strains similar to the Wuhan virus, Delta, Alpha and Omicron were developed and cloned into the pFastBac plasmid. The proteins were synthesized in the baculovirus expression system and assembled into VLP in the portable Trichoplusia ni cell. The presence of insertion in the baculovirus genome was determined by PCR. ELISA and immunoblotting were used to study the antigenic activity of VLP. VLP purification was performed by ultracentrifugation using 20% sucrose. Morphology was assessed using electron microscopy and dynamic light scattering. Results. VLPs consisting of recombinant SARS-CoV-2 proteins (S, M, E and N) were obtained and characterized. The specific binding of antigenic determinants in synthesized VLPs with antibodies to SARS-CoV-2 proteins has been demonstrated. The immunogenic properties of VLPs have been studied. Conclusion. The production and purification of recombinant VLPs consisting of full-length SARS-CoV-2 proteins with a universal set of surface antigens have been developed and optimized. Self-assembling particles that mimic the coronavirus virion induce a specific immune response against SARS-CoV-2.

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Molecular epidemiological analysis of SARS-CoV-2 genovariants in Moscow and Moscow region

Cited by 2 publications

References 29 publications

COVID-19 Epidemic in the Kaliningrad Region: Incidence and Infection Control Measures

COVID-19 Epidemic in the Kaliningrad Region: Incidence and Infection Control Measures

Development, production and characterization of SARS-CoV-2 virus-like particles (Coronaviridae: <i>Orthocoronavirinae: Betacoronavirus: Sarbecovirus</i>)

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