Molecular epidemiological investigation of group A porcine rotavirus in East China

Abstract

Group A porcine rotavirus (RVA) is a serious threat to the breeding industry worldwide, which was associated with severe diarrhea in piglets. However, the prevalence and molecular characterizations of RVA circulating in farms of East China remains largely unknown. Five hundred and ninety-four samples were collected from 35 farms in East China from September 2017 to December 2019. The results showed that 16.8% was positive for RVA of all samples. Among different types of samples, the highest positive rate of RVA was intestinal samples (19.5%), and among pigs at different growth stages, the highest detection rate of RVA in piglets was 18.5%. Furthermore, the VP7 and VP4 genes of nine positive samples were sequenced for alignment and phylogenetic analysis. Phylogenetic analysis revealed that the nine isolates belong to four kinds of genotype combinations correspondingly: G9P[7](5/9), G5P[13](2/9), G9P[13](1/9), and G5P[7](1/9).The data suggested that multiple genotypes combinations of RVA were circulating in pigs in East China. Thus, it's necessary to continuously survey the prevalence of RVA in pigs, aiding the rational application of vaccines or other measures for the prevention and control of RVA spread.

Epidemiological monitoring and genetic variation analysis of pathogens associated with porcine viral diarrhea in southern China from 2021 to 2023

“…PoRV is the second most common viral pathogen causing diarrhea in pigs. The 13 PoRV strains in this study were dominated by the G9 genotype, accounting for 61.54% of the total, followed by the G5 genotype, accounting for 15.38% of the total, which was consistent with the results of the previous study ( Tao et al, 2023 ). Papp et al collected and screened 77 original articles from various regions between 1976 and 2011 to investigate the distribution of porcine rotavirus group A genotypes.…”

Development of a multiplex reverse transcription-quantitative PCR (qPCR) method for detecting common causative agents of swine viral diarrhea in China

“…Our results presented in this study demonstrated a complex condition on the prevalence of RVA on Chinese pig farms, as evidenced by the characterization of 10 RVA genotypes. Among these genotypes, G9, G5, and G4 were the predominantly characterized genotypes, which agrees well with the other reports from both China and other countries [ 41 , 42 ], indicating an active role these genotypes in promoting swine diarrhea.…”

Recent Molecular Characterization of Porcine Rotaviruses Detected in China and Their Phylogenetic Relationships with Human Rotaviruses

“…Epidemiological survey data have shown that PoRVA is endemic and widespread in large-scale pig farms worldwide [ 15 , 16 ]. Until now, there has been no nationwide epidemiological investigation specifically targeting multiple genes of PoRVA in China, except a few studies that reported the positive rate of pig samples and farms in individual provinces [ 21 , 22 , 23 ]. For instance, a study conducted from 2013 to 2019 reported an RVA positive rate of 7% in Zhejiang, Shandong, Jiangsu and Shanxi provinces [ 24 ].…”

“…For instance, a study conducted from 2013 to 2019 reported an RVA positive rate of 7% in Zhejiang, Shandong, Jiangsu and Shanxi provinces [ 24 ]. Another study conducted from 2017 to 2019 found an RVA positive rate of 16.83% (100/394) in East China [ 23 ]. In 2022, a study conducted in Heilongjiang province reported an RVA positive rate of 4.3% (12/280) [ 25 ].…”