Molecular epidemiology and antifungal susceptibility of<i>Saprochaete capitata</i>(<i>Blastoschizomyces capitatus</i>) isolates causing nosocomial infection in Kayseri/Turkey

Koç, Ayşe; Atalay, Mustafa; Timur, Demet; Demir, Gonca; Kaynar, Leylagül

doi:10.1080/23744235.2016.1176246

Cited by 13 publications

(6 citation statements)

References 33 publications

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“…However, microbiological investigations of the hospital environment failed to find the isolate. 26 While M capitatus is considered a ubiquitous environmental organism and part of the normal human gastrointestinal flora, 18 to the authors’ knowledge, there has not been any case reports or studies tracing M capitatus to a hospital fomite. More studies are needed to determine a true correlation between ICU admissions and M capitatus infections.…”

Section: Discussionmentioning

confidence: 98%

A Case of Magnusiomyces capitatus Peritonitis Without Underlying Malignancies

D’Assumpcao

Lee

Heidari

2018

Journal of Investigative Medicine High Impact Case Reports

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Magnusiomyces capitatus is a rare cause of fungal infection in immunocompromised patients, mainly seen in hematological malignancies. M capitatus infections are extremely rare in immunocompetent patients, as it is part of normal human microbial flora. We are presenting an extremely rare case of M capitatus peritonitis in an otherwise immunocompetent patient who suffered from gastrointestinal leakage due to pancreatitis. Fungal identification was performed at reference laboratory by phenotypic characteristics and DNA sequencing of target internal transcribed spacer region of the rRNA gene and the D1-D2 domain of the large-subunit rRNA gene and susceptibility testing by Clinical and Laboratory Standards Institute guidelines (document M27-S4) broth dilution method. He was successfully treated with a combination of surgical repair and voriconazole single therapy.

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Section: Discussionmentioning

confidence: 98%

A Case of Magnusiomyces capitatus Peritonitis Without Underlying Malignancies

D’Assumpcao

Lee

Heidari

2018

Journal of Investigative Medicine High Impact Case Reports

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“…Vaux et al (2) assessed the genomic single nucleotide polymorphisms of 73 S. clavata isolates using a mapping approach and obtained two largely homogeneous phylogenetic sublineages. In a recent study, Koç et al (27) evaluated clinical isolates of M. capitatus using repetitive element sequence-based-PCR fingerprinting. On the basis of cluster similarity percentages, five main clones were detected; however, no genetic relationship was observed according to the patients' identity, location, or sample type.…”

Section: Discussionmentioning

confidence: 99%

7 / Molecular diagnostics of arthroconidial yeasts– frequent pulmonary opportunists

Kaplan¹

2018

Preprint

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Magnusiomyces capitatus and Saprochaete clavata are members of the clade of arthroconidial yeasts that represent emerging opportunistic pulmonary pathogens in immunocompromised patients. Given that standard ribosomal DNA (rDNA) identification often provides confusing results, in this study, we analyzed 34 isolates with the goal of finding new genetic markers for classification using multilocus sequencing and amplified fragment length polymorphism (AFLP). The interspecific similarity obtained using rDNA markers (the internal transcribed spacer [ITS] and large subunit regions) was in the range of 96 to 99%, whereas that obtained using protein-coding loci (Rbp2, Act, and Tef1␣) was lower at 89.4 to 95.2%. Ultimately, Rbp2 was selected as the best marker for species distinction. On the basis of cloned ITS data, some strains proved to be misidentified in comparison with the identities obtained with phenotypic characters, protein sequences, and AFLP profiles, indicating that different copies of the ribosomal operon were present in a single species. Antifungal susceptibility testing revealed that voriconazole had the lowest MIC against M. capitatus, while amphotericin B had the lowest MIC against S. clavata. Both species exhibited in vitro resistance to fluconazole and micafungin.

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“…In report from Spain, 20 out of 25 acute leukemia patients had fungemia with visceral dissemination with 30-day mortality of 52% (26). In a report from Turkey, from 2012 to 2015, a total of 20 S. capitata strains were isolated from onco-haematological and ICU patients in a university hospital (27). It was involved with many outbreaks and contaminated dairy products (25).…”

Section: Saprochaete Capitata (Bastoschzomyces Capitatus)mentioning

confidence: 99%

Rare yeasts: emerging threat in immunocompromised patients

Kapmaz

Tekin

Doğan

et al. 2019

Infect Dis Clin Microbiol

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Fungemia due to the yeasts other than Candida is a clinically rare, but still emerging entity. The clinical terminologies of rare opportunistic yeast bloodstream infections (ROYBSIs) or micafungin breakthrough fungemia (MBF) or echinocandin non-susceptible yeast (ENSY) refer mostly to the same entity. Clinicians should be alert about the early distinction. ROYBSIs are mostly resistant to echinocandins and should be taken into consideration when yeast growth in blood culture of a patient previously treated with an antifungal, especially an echinocandin. Apart from molecular methods, MALDI-TOF might be considered as the most useful commercial system for identification of ROYBSIs. A combination of L-AmB and voriconazole is the appropriate empirical therapy for MBF, especially in neutropenic patients.

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Molecular epidemiology and antifungal susceptibility ofSaprochaete capitata(Blastoschizomyces capitatus) isolates causing nosocomial infection in Kayseri/Turkey

Abstract: S. capitata isolates are an important cause of nosocomial infection in the HBMU and ICUs.

Cited by 13 publications

References 33 publications

A Case of Magnusiomyces capitatus Peritonitis Without Underlying Malignancies

A Case of Magnusiomyces capitatus Peritonitis Without Underlying Malignancies

7 / Molecular diagnostics of arthroconidial yeasts– frequent pulmonary opportunists

Rare yeasts: emerging threat in immunocompromised patients

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