Molecular epidemiology and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolates in Xiangyang, China

Tang, Y.W.; Cao, Rui; Xiao, Na; Li, Zhishan; Wang, Rong; Zou, Jiu-Ming; Pei, Jiao

doi:10.1016/j.jgar.2017.08.016

Cited by 14 publications

(13 citation statements)

References 35 publications

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“…However, hospital-associated SCCmec types were also found in this study, indicating a cross transmission between the community and hospital facilities. Moreover, the dominant STs (ST5, ST45, ST59 and ST188) in this study were consistent with other community-based populations [26,[35][36], but some other STs were also previously reported in hospital patients (ST338 and ST1) [37][38][39] and animals ( ST398 and ST1) [40][41][42]. The MLST findings also indicated the cross transmission between communities and hospital settings.…”

Section: Discussionsupporting

confidence: 92%

Prevalence and molecular epidemiology of methicillin-resistant Staphylococcus aureus colonization with and without methicillin-resistant coagulase-negative Staphylococci in HIV-infected patients

Liu¹,

Lin²,

Li³

et al. 2020

Preprint

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Background The global prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization in human immunodeficiency virus (HIV)-infected patients is increasing, but data about it was limited in mainland China. This study aims to investigate the epidemiology of MRSA nasal colonization among HIV-infected patients in mainland China and also evaluate the impact of methicillin-resistant coagulase-negative Staphylococci (MRCoNS). Methods This cross-sectional study was designed to collect nasal samples and individual information for HIV-infected participants. Risk factors and phenotypic and molecular characteristics among those with MRSA colonization comparing those with and without MRCoNS co-colonization were analyzed.Results We found 119 (11.89%) out of 1001 HIV-infected patients were colonized with MRSA, including 41 (4.10%) with MRCoNS and 78 (7.79%) without MRCoNS. Having a history of respiratory tract infection in the previous 6 months(adjusted OR=1.64, 95% CI: 1.04-2.57) and male gender (aOR=3.92, 95% CI: 1.20-12.85) were risk factors for the overall MRSA and MRSA without MRCoNS colonization, respectively. No risk factor was associated with co-colonization of MRSA and MRCoNS. The proportions of antibiotic resistance and toxin genes as well as the distribution of molecular types for MRSA isolates were equivalent between subjects with and without MRCoNS isolates (P-value ≥0.05). Conclusions HIV-infected patients remain a highly vulnerable population for MRSA colonization, and men and prior history of respiratory tract infection are risk factors. Risk factors and phenotypic and molecular characteristics of MRSA colonization were not affected by co-colonizing with MRCoNS.

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Section: Discussionsupporting

confidence: 92%

Prevalence and molecular epidemiology of methicillin-resistant Staphylococcus aureus colonization with and without methicillin-resistant coagulase-negative Staphylococci in HIV-infected patients

Liu¹,

Lin²,

Li³

et al. 2020

Preprint

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“…The aforementioned erava MIC data differ from the reported data previously showing higher erava MICs in MRSA than MSSA [3, 4]. These differences could be due, at least in part, to sample and regional variation given that the molecular and antimicrobial susceptibility characteristics of S. aureus are known to vary across regionsand the very limited volume of data that have been reported regarding erava effects on S. aureus [28, 29]. Previously, there has been a focus on the antimicrobial susceptibility of erava mainly in the multi-drug resistant S. aureus , including MRSA as well as vancomycin-resistant and linezolid-resistant isolates.…”

Section: Discussionmentioning

confidence: 68%

“…Erava, being a new Tet class drug, has not yet completed its phase III clinical trial and does not yet have its own susceptibility breakpoints recommended by CLSI [3–7, 24]. Consequently, we referred conservatively to the tigecycline susceptibility MIC breakpoint for S. aureus of 0.5 mg/L, which was derived from the US Food and Drug Administration [24–26, 28]. The available data thus far suggest that erava is two- to four-fold more active than tigecycline against common clinically important Gram-positive aerobic species [3, 4].…”

Section: Discussionmentioning

confidence: 99%

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Eravacycline activity against clinical S. aureus isolates from China: in vitro activity, MLST profiles and heteroresistance

Zhang

Bai

et al. 2018

BMC Microbiol

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BackgroundMortality rates for patients with Staphylococcus aureus (S. aureus) infections have improved only modestly in recent decades and S. aureus infections remain a major clinical challenge This study investigated the in vitro antimicrobial activity of erevacycline (erava) against clinical S. aureus isolates from China, as well as the heteroresistance frequency of erava and sequence types (STs) represented in the sample.ResultsA sample of 328 non-duplicate clinical S. aureus isolates, including 138 methecillin-resistant (MRSA) and 190 methecillin-sensitive (MSSA) isolates, were collected retrospectively in China. Erava exhibited excellent in vitro activity (MIC50 ≤ 0.25 mg/L) against MRSA and MSSA, including isolates harboring Tet specific resistance genes. The frequency of erava heteroresistance in MSSA with erava MICs = 0.5 mg/L was 13.79% (4/29); no MRSA with erava MICs ≤0.5 mg/L exhibited heteroresistance. Heteroresistance- derived clones had no 30S ribosome subunit mutations, but their erava MICs (range, 1–4 mg/L) were suppressed dramatically in the presence of efflux protein inhibitors.ConclusionsConclusively, erava exhibited excellent in vitro activity against S. aureus, however hints of erava heteroresistance risk and MIC creep were detected, particularly among MSSA with MICs of 0.5 mg/L.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1349-7) contains supplementary material, which is available to authorized users.

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“…In our study, more than 50% of the isolates from both hospitals were identified as MRSA, and this rate was similar to the detection rate in other cities in China. 15,16 All of the strains exhibited high resistance against penicillin, erythromycin, and clindamycin. However, no strains were resistant to vancomycin, linezolid, or nitrofurantoin, indicating that these drugs were still effective in treating orthopedic infections caused by S. aureus isolates and could be the first-choice drugs for treating severe MRSA infections.…”

Section: Discussionmentioning

confidence: 99%

Pathogenic characteristics of Staphylococcus aureus isolates from arthroplasty infections

et al. 2020

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Background: Staphylococcus aureus has a great ability to form biofilms on implant-related biomaterials. This study aimed to investigate the resistance, biofilm and molecular characteristics of S. aureus strains isolated from patients with postoperative infections after arthroplasty in two Chinese tertiary care hospitals during 2017 to 2018. Methods: Antimicrobial susceptibility was determined by the agar dilution method. Bacterial biofilm formation was determined by crystal violet staining. The genes related to biofilm formation and molecular typing were analyzed by PCR amplification. Results: A total of 33 isolates were collected, 21 of which were from Henan. The strains were completely sensitive to vancomycin, linezolid, and nitrofurantoin. All the isolates had adhesion ability and could produce biofilms. Of the isolates, 75.0% from Chongqing and 85.7% from Henan had stronger biofilm formation abilities. The strains from Henan had slightly higher resistance, adhesion and biofilm-forming abilities than those from Chongqing. The strains in both hospitals carried at least two genes related to biofilm formation, and the ica and fnb genes were the most frequently detected genes. Three SCC mec types and seven sequence types (STs) were found in Henan, and two SCC mec types and six STs were found in Chongqing. ST239-SCC mec III was the main epidemic clone in the two hospitals. Conclusion: The resistance phenotype and molecular characteristics of S. aureus strains varied in different hospitals. The results reflect the potential risks of S. aureus infection in postoperative arthroplasty patients. Our study provides a powerful basis for the clinical treatment, infection control and monitoring of outbreaks of epidemic strains.

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Molecular epidemiology and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus isolates in Xiangyang, China

Cited by 14 publications

References 35 publications

Prevalence and molecular epidemiology of methicillin-resistant Staphylococcus aureus colonization with and without methicillin-resistant coagulase-negative Staphylococci in HIV-infected patients

Prevalence and molecular epidemiology of methicillin-resistant Staphylococcus aureus colonization with and without methicillin-resistant coagulase-negative Staphylococci in HIV-infected patients

Eravacycline activity against clinical S. aureus isolates from China: in vitro activity, MLST profiles and heteroresistance

Pathogenic characteristics of Staphylococcus aureus isolates from arthroplasty infections

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