Molecular epidemiology and antimicrobials resistance mechanism of Mycoplasma genitlaium

Abstract

Currently, infections caused by Mycoplasma genitalium are ones the most common sexually transmitted infections. Their prevalence is varied from 1.3% to 15.9%. Infections caused by M.genitalium may lead to urethritis in men and a wide spectrum of diseases in women. Antibiotic resistance now is one of the most emerging problems both in the scientific and in the healthcare fields. The usage of antimicrobials inhibiting cell wall synthesis for the treatment of M.genitalium is ineffective, and resistance to macrolides and fluoroquinolones is increasing rapidly. M.genitalium infections diagnostics is complicated due to specific conditions and duration of culture methods. The usage of nucleic acid amplification techniques is the most relevant for laboratory diagnostics, and is used in existing assays. This review compiles current data on the prevalence, molecular mechanisms of pathogenesis and antibiotic resistance, as well as diagnostics methods of M.genitalium.

Detection of macrolide and fluoroquinolone resistance-associated 23S rRNA and parC mutations in Mycoplasma genitalium by nested real-time PCR

“…However, its value in clinical applications is limited by the complexity of the processes and the lack of professional instrumentation in some laboratories. Although real-time fluorescent PCR-based commercial kits or self-constructed methods are available and evaluated, they usually have limitations, such as detecting only one single target, either 23S rRNA or parC, lack of accurate genotype identification and unpublished primer and probe sets (Wold et al, 2015;Thellin et al, 2019;Le Roy et al, 2020;Gullsby et al, 2021;Shedko et al, 2021). To provide a flexible and rapid guide to clinical antibiotic therapy, this study aims to establish a nested real-time PCR assay for key sites associated with macrolide and fluoroquinolone resistance, including mutations at 23S rRNA A2058, A2059 and parC S83, D87 positions.…”