“…The capsid-encoding VP3 and VP1 genes and the 2A gene of enterovirus were amplified with PCR primers 008 (sense, 5′-GCRTGCAATGAYTTCTCWGT-3′, nt 2411-2430, PV1-Mahoney) and 011 (antisense, 5′-GCIC-CIGAYTGITGICCRAA-3′, nt 3408-3389, PV1-Mahoney) (9). PCR amplification was done for 35 cycles at 94°C for 30 s, 51°C for 30 s, and 72°C for 30 s. The RT-PCR products were analyzed by electrophoresis on 2% agarose gels containing 0.5 µg/mL of ethidium bromide, and purified by using a gel extraction kit (Bioasia Co., Shanghai, China).…”