Molecular Epidemiology and In Vitro Antifungal Susceptibility of Candida Isolates from Women with Vulvovaginal Candidiasis in Northern Cities of Khuzestan Province, Iran

Hasanvand, Shirin; Qomi, Hamid Azadegan; Kord, Mohammad; Didehdar, Mojtaba

doi:10.5812/jjm.12804

Cited by 22 publications

(25 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite C. glabrata is reported to be naturally resistant species to fluconazole or related azole antifungals [ 37 ], all the three vaginal isolates of C. glabrata in the current study were susceptible to fluconazole and other azole antifungals. Our finding is more or less concurrent with the findings of Hasanvand et al [ 34 ] in which out of 19 vaginal C. glabrata isolates only three isolates were resistant to fluconazole. Similarly, Richter et al [ 39 ] documented that out of 112 isolates of C. glabrata only 67% (51.8% susceptible- dose- dependent, 15.2% resistant) was fluconazole non-susceptible.…”

Section: Discussionsupporting

confidence: 92%

“…The study of Bauters et al [ 29 ] showed that C. glabrata as the most commonly isolated non -albicans Candida species (16.3%), followed by, C. parapsilosis (8.9%), C. humicola (1.6%), C. krusei (0.8%), and C. lusitaniae (0.8%). Hasanvand et al [ 34 ] demonstrated that C. albicans as the most commonly isolated species followed by C. glabrata, C. tropicalis , and C. parapsilosis. In contrast to these reports, C. krusei was the dominant non- albicans Candidia species in the present study accounting for 17.2% of the total isolates.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Vulvovaginal candidiasis: species distribution of Candida and their antifungal susceptibility pattern

2018

View full text Add to dashboard Cite

BackgroundVulvovaginal candidiasis is a global issue of concern due to its association with economic costs, sexually transmitted infections, and ascending genital tract infection. The aim of this study was to determine species distribution and antifungal susceptibility pattern of Candida species causing vulvovaginal candidiasis.MethodsA cross sectional study was conducted from November 2015 to December 2016 at the Family Guidance Association of Ethiopia. Vaginal swabs collected from study subjects that were clinically diagnosed with vulvovaginal candidiasis were cultured. Yeast identification and antifungal susceptibility testing were determined by the automated VITEK 2 compact system. The association of vulvovaginal candidiasis with possible risk factors was assessed and analyzed using SPSS version 20.ResultsThe overall prevalence of vulvovaginal candidiasis was 41.4%. The association of vulvovaginal candidiasis was statistically significant with previous genital tract infection (p = 0.004), number of life-time male sex partners (p = .037), and number of male sex partners in 12 month (p = 0.001). Of 87 Candida isolates recovered, 58.6% were C. albicans while 41.4% were non-albicans Candida species. The highest overall drug resistance rate of Candida species was observed against fluconazole (17.2%), followed by flycytosine (5.7%). All Candida isolates were 100% susceptible to voriconazole, caspofungin, and micafungin. C. albicans, was 100% susceptible to all drugs tested except fluconazole and flycytosine with a resistance rate of 2% each drug. C. krusei, was 100 and 33.3% resistant to fluconazole and flycytosine, respectively.ConclusionsHigh prevalence rate of vulvovaginal candidiasis and observation of high prevalence rate of non-albicans Candida species in the present study substantiate, the importance of conducting continuous epidemiological surveys to measure changes in species distribution from C. albicans to non-albicans Candida species in Ethiopia. Although, fluconazole still appeared to be active against all isolates of C. albicans and non-albicans Candida species high resistance rate of C. krusei against the drug may demonstrate a search for alternative antifungal drugs when treating vulvovaginal candidiasis caused by C. krusei.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Vulvovaginal candidiasis: species distribution of Candida and their antifungal susceptibility pattern

2018

View full text Add to dashboard Cite

show abstract

“…On the other hand, contrary to the notion that C. glabrata is intrinsically non-susceptible to fluconazole, all isolates in our study were sensitive to all azole drugs tested. More or less similar results were reported by many studies [17,27,43].…”

Section: Discussionsupporting

confidence: 89%

Distribution of Candida albicans and non-albicans Candida species isolated in different clinical samples and their in vitro antifungal suscetibity profile in Ethiopia

2020

View full text Add to dashboard Cite

Background: The spectrum of yeasts and their antifungal susceptibility profile are poorly known and treatment of fungal disease has remained empirical. The aim of this study is to determine the spectrum and antifungal susceptibility profile of yeasts particularly of Candida species.Methods: A descriptive study on the composition of Candida species and antifungal susceptibility profile were conducted from January 2018 to September 2018. Clinical samples collected from different sites were cultured on Sabouraud dextrose agar and incubated for an appropriate time. Identification of yeast isolates and their antifungal susceptibility profile were determined by the VITEK 2 compact system. Descriptive statistics such as frequency and percentage of Candida species were calculated using SPSS version 20.Results: Of 209 yeasts recovered, 104(49.8%), 90 (43.1%), 15(7.2%) were C. albicans, non albicans Candida species, and other yeasts, respectively. Among non albicans Candida species, Candida krusei was the commonest isolate. Of other yeast groups, 66.7% was represented by Cryptococcus laurentii. Regardless of Candida species identified, 85.6, 3.9, and 10.5% of the isolates were susceptible, intermediate, and resistant to fluconazole, respectively. C krusei was 100% resistant to the drug. Voriconazole demonstrated the greatest antifungal activity against Candida isolates in which 99.4% of Candida isolates were susceptible. The susceptibility and the resistance rate of Candida isolate to both caspofungin and micafungin were the same being 96 and 4% respectively. However, micafungin was more potent than caspofungin. The susceptibility, resistant, and intermediate rates of yeasts against flucytosine were, 86.2, 6.6, and 7.2%, respectively. Conclusions:The present study demonstrated the distribution of Candida species in different clinical specimens where the isolation rate of non-albicans Candida species was comparable to Candida albicans. The high resistance rate of C. krusei to fluconazole and flucytosine may demonstrate that the treatment of candidiasis empirically is questionable.

show abstract

“…Compared with the other studies in Iran [9,17,20], the lowest rate of dermatophyte species was isolated in the current study which can be due to the hygiene of the swimmers of our considered pools. In the present study, only one case of Trichophyton rubrum was isolated from foot-washing sink.…”

Section: Discussioncontrasting

confidence: 84%

“…Filamentous fungal isolates were identified by slide culture method [19]. Yeast fungal isolates were subcultured on CHROMagar™ Candida medium (CHROMagar, France) and incubated at 35°C for 48 h. Discrimination of Candida species and other yeast species was done according to different colors produced by Candida colonies in the chromogenic medium according to the manufacturer's protocol [20].…”

Section: Mycological Investigationsmentioning

confidence: 99%

Fungal and parasitic contamination of indoor public swimming pools in Arak, Iran

Sarmadian

Hazbavi²,

Didehdar³

et al. 2020

J. Egypt. Public. Health. Assoc.

Self Cite

View full text Add to dashboard Cite

Background: Swimming is a popular exercise for different types of people at different ages. Public swimming pools are places where fungal infections can be easily transferred. The purpose of this study is to evaluate the quality of mycological, parasitological, and physicochemical parameters of swimming pools of Arak city. Methods: This cross-sectional study was done for 12 months from April 2013 to March 2014 in six indoor active swimming pools of Arak city (A, B, C, D, E, and F). Samples were collected in four seasons, two times/season; each time, two samples were obtained from six specified locations (shallow level pool, deep level pool, dressing rooms, showers, margin of pool walls, and foot-washing sink) from each pool with a total of 576 samples. Physicochemical parameters including water temperature, pH, turbidity, and the residual chlorine were measured on-site. In order to isolate and detect the fungal agents, special filters and culture Sabouraud's dextrose agar, chloramphenicol, and mycosel agar media were applied. Furthermore, non-nutrient agar medium enriched with Escherichia coli was used to detect and to separate the eggs of the worms, cysts, and parasitic protozoa from centrifuges of samples. In order to investigate their sediment, optical microscope and culture media were used. Results: We found that 456 (79.1%) samples were positive regarding the fungal elements, and 516 fungal species were isolated. The most common isolates were saprophytic species (8.74%), yeast species (25%), and dermatophyte species (2.5%). The most contaminated surfaces were foot-washing sinks and showers. In this study, Acanthamoeba parasites were isolated from one pool only. All the investigated physicochemical parameters of pool water except for the temperature were found to be in the standard range. Conclusions: Existence of saprophytic fungi and yeast in pools' water is plausible to be considered as an indicator of water resistance to the detergent agents. This high degree of contamination is due to the huge number of visitors, the complexity of construction, the choice of materials, and the long opening hours. Isolation of dermatophytes and Acanthamoeba parasite from the pools' area and foot-washing sink reveals the important role of the public swimming pools in disease transmission.

show abstract

Molecular Epidemiology and In Vitro Antifungal Susceptibility of Candida Isolates from Women with Vulvovaginal Candidiasis in Northern Cities of Khuzestan Province, Iran

Cited by 22 publications

References 32 publications

Vulvovaginal candidiasis: species distribution of Candida and their antifungal susceptibility pattern

Vulvovaginal candidiasis: species distribution of Candida and their antifungal susceptibility pattern

Distribution of Candida albicans and non-albicans Candida species isolated in different clinical samples and their in vitro antifungal suscetibity profile in Ethiopia

Fungal and parasitic contamination of indoor public swimming pools in Arak, Iran

Contact Info

Product

Resources

About