Molecular epidemiology and phylogenetic analysis of human papillomavirus infection in women with cervical lesions and cancer from the coastal region of Ecuador

Bedoya-Pilozo, César; Magües, Lex G. Medina; Espinosa-García, Maylen; Sanchez, M; Valdiviezo, Johanna V. Parrales; Molina, Denisse; Ibarra, María A.; Quimis-Ponce, María; España, Karool; Macias, Karla E. Párraga; Flores, Nancy; Orlando, Solon Alberto; Penaherrera, Jorge A. Robalino; Chedraui, Peter; Escobar, Saul; Loja-Chango, Rita; Ramírez-Morán, Cecibel; Espinoza-Caicedo, Jasson; Sánchez-Giler, Sunny; Limia, Celia M.; Alemán, Yoan; Soto, Yudira; Kourí, Vivian; Culasso, Andrés Carlos Alberto; Badano, Inés

doi:10.1016/j.ram.2017.06.004

Cited by 20 publications

(29 citation statements)

References 40 publications

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“…Of the 144 participants recruited in this study 16.9% were positive for HPV type 16, which is slightly higher than the study carried out in Kano, Nigeria where they reported prevalence of 15.8% for HPV type 16, though their study recruited lesser subjects (50) than this study [13]. A study carried out in Lagos, Nigeria, which they reported HPV type 16 to be 46.9%, this is not in accordance with the present study [20], another study reported 23.5% [21], while in a study carried out in the south western Nıgeria HPV 16 prevalence (3.5%) was very low than the one in this study [22]. with P value of 0.14 [24].…”

Section: Resultscontrasting

confidence: 99%

“…This study shows there is an association between infection with HPV 16 and cervical lesions, this is consistent with a study where the strength of the association was even demonstrated with odd ratio of 182, showing a strong association between HPV 16 and cervical lesions[23]. While another study also showed HPV 16 is a strong risk factor for cervical lesion[21].Symptoms such as genital itching, vaginal discharge (also known PV discharge), post-coital bleeding, are the most common symptoms seen in women with cervical abnormalities and vaginal infections. There was no complain of post-coital bleeding among the participants in this study, but all the subjects that were positive for HPV type 16 complained of either genital…”

supporting

confidence: 91%

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Identification of HPV16 in suspected cases of cervical lesions and docking Study of its L1 protein with some natural inhibitors.

Lukman

Bala

Malik

et al. 2020

Preprint

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Background The Human papillomavirus (HPV) causes sexually transmitted diseases. Among several types of HPV variants, HPV 16 is listed as a high-risk group, the primary cervical cancer etiologic agent, which causes life-threatening disease among women worldwide. The presence of L1, E6 and E7 encoded oncoproteins are largely responsible for virulence and pathogenicity that leads to cervical lesions. This menace is required to be curbed by designing an anti-cancerous drugs. The protein receptor-inhibitor interaction adopted using in silico analysis is very important in drug designing. It was the objective of this study to identify HPV16 isolates from suspected cases of cervical cancer at SH Sokoto and SYMH Birnin Kebbi hospitals and also to identify potent HPV16’s L1 protein inhibitor using in silico analysis of Echinacoside, curcumin and Cichoric acid against the viral protein. Methods A total of 140 cervical smear samples consisting of 21 low grade squamous intraepithelial lesion, 6 high grade lesion and 117 negative pap smears were collected. The samples were subjected for molecular detection using PCR targeting E6 and L1 genes of the virus. Positive samples were sequenced using Sanger sequencing platform. All the sequencing data were analysed using bioedit software while data generated for the molecular prevalence was statistically analyzed using Chi-square. A comprehensive HPV L1 protein homology model was designed to predict the L1 protein interaction mechanism with natural inhibitory molecules using a structural drug design approach. AutoDock Vina was used to carry out the molecular docking. Results Out of the 140 samples, 24 samples were positive for the PCR representing 16.7% molecular prevalence rate. There is statistically significant association between cyto-diagnoses and presence of HPV16 ( P ˂0.05). The highest prevalence rate of 12(50% of positive sample) was recorded among women between 30-39 years old. Docking analysis showed that the Chicoric acid components of Echinacea purpurae have strong binding affinity to the L1 protein of the HPV. Conclusion This study provides data on HPV 16 epidemiology in northern Nigeria, High-risk type 16 HPV variant was identified and also provides novel evidence for consideration on certain interacting residues, when synthesizing Anti-HPV compounds in the wet lab.

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Section: Resultscontrasting

confidence: 99%

supporting

confidence: 91%

Identification of HPV16 in suspected cases of cervical lesions and docking Study of its L1 protein with some natural inhibitors.

Lukman

Bala

Malik

et al. 2020

Preprint

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“…De acuerdo con los pocos estudios realizados en América del Sur, además del VPH 16 y el 18, el VPH 58 es otro de los VPH-AR encontrados con mayor frecuencia en la región. Este último ha sido detectado en el centro y norte de Brasil, Argentina, Colombia y Ecuador 5 .…”

Section: Introductionunclassified

Infección por Virus del Papiloma Humano y citología cérvico-vaginal en mujeres indígenas del Cañar, Ecuador.

Ordóñez

Brito

Antúnez

et al. 2019

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There are few studies on the circulation of Human Papilloma Virus (HPV) in indigenous Ecuadorian women. The aim of the study is to determine the circulation of HPV and identify cytological alterations in cervical samples of indigenous Ecuadorian women and to define the behavior of some socio-demographic and clinical-epidemiological variables. An analytical cross-sectional study was done between July 2017 and September 2018 to determine the presence of cytological alterations and HPV infection in 100 indigenous women between 15 and 55 years of age, residing in Cañar, Ecuador. The association between socio-demographic and clinical-epidemiological variables with viral infection was investigated. Was obtained a 98% of negative cytology for malignancy and 2% of cervical lesions. Cases with high-grade lesions were not diagnosed. In general, a 34% (34/100) tested was positive for HPV, predominating oncogenic genotypes. HPV 31 was the most frequent in 41.2% (14/34) of cases followed by HPV 16 in 20.6% (7/34). Women between 20 and 30 years of age were five times more likely to be infected with HPV (44.1%, 15/34). The frequency of infection was significantly higher in single women and in those who reported having 2 to 3 births. Infection with HPV 16 was associated with the use of hormonal contraceptives, in 57.1% (4/7) of the cases; p = 0.005, RP = 12.44 IC95% (2.40-64.62). The high prevalence of oncogenic HPV infection indicates the need to incorporate this indigenous population into early detection programs for cervical cancer.

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“…The objective of this study is to show the prevalence of HPV genotypes in Ecuadorian mestizo women [13], in comparison with other studies at Latin America [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] (see table 3).…”

Section: Introductionmentioning

confidence: 99%

Diagnostic screening of HPV genotypes in 555 Ecuadorian mestizo women of seven provinces, and comparison with other Latino American populations

González-Andrade¹,

Serrano²,

Pinos³

et al. 2019

archmed

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Objective: this paper aims to perform diagnostic screening of HPV in healthy Ecuadorian mestizo women, from seven provinces, and compare the findings with other Latin American populations. Material and methods: genotyping was done with two different oligonucleotides MY09 and MY11; a fragment of 450 base pairs was amplified, L1 region of the viral genome. Results: it analyzed 555 women, 35 were positive for HPV (6.3%). Genotypes found in relation to oncogenic risk, were 6, 11, 16, 18, 31, 33, 35, 42, 45, 51, 52, 53, 58, 59, 61, 81. 12/35 women (34.3%) presented high-risk genotypes. Four positive cases were also observed in women older than 55 years (0.36%). The 14 published studies of Ecuadorian women showed that the most prevalent genotypes are 16, 18, 31, 52, 53, 56 and 58; while in the eight Latin American Studies the most prevalent are 16, 18, 31, 45, 52 and 58. Conclusion: although there are several studies on HPV genotyping on Latin American populations, there is an important gap related to ethnicity and the prevalence of the virus. In addition, most of them have not compared similar and common subtypes in the population. The general prevalence of HPV in the studied population was 6.3%. It found that genotypes 16, 18, 31, 52, 53, 56 and 58 are the most prevalent in Ecuadorian normal mestizo women. Genotypes 53 and 56 are common in Latino populations. Larger studies, in different ethnic groups are needed to identify other prevalent genotypes in certain geographical areas.

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Molecular epidemiology and phylogenetic analysis of human papillomavirus infection in women with cervical lesions and cancer from the coastal region of Ecuador

Cited by 20 publications

References 40 publications

Identification of HPV16 in suspected cases of cervical lesions and docking Study of its L1 protein with some natural inhibitors.

Identification of HPV16 in suspected cases of cervical lesions and docking Study of its L1 protein with some natural inhibitors.

Infección por Virus del Papiloma Humano y citología cérvico-vaginal en mujeres indígenas del Cañar, Ecuador.

Diagnostic screening of HPV genotypes in 555 Ecuadorian mestizo women of seven provinces, and comparison with other Latino American populations

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