Molecular epidemiology, antibiotic resistance profile and frequency of integron 1 and 2 in adherent-invasive Escherichia coli isolates of colorectal cancer patients

Heidari, Aida; Emami, Mohammad Hassan; Maghool, Fatemeh; Mohammadzadeh, Samane; Kadkhodaei Elyaderani, Parisa; Safari, Tahereh; Fahim, Alireza; Kamali Dolatabadi, Razie

doi:10.3389/fmicb.2024.1366719

Cited by 3 publications

References 65 publications

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Comparison of Crohn’s disease-associated adherent-invasive Escherichia coli (AIEC) from France and Hong Kong: results from the Pacific study

Chevarin,

Xu,

Martin

et al. 2024

Gut Microbes

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Association between ileal colonization by Adherent-Invasive Escherichia coli (AIEC) and Crohn’s disease (CD) has been widely described in high-incidence Western countries but remains unexplored in Asian countries with a fast increase in CD incidence. In the PACIFIC study, we compared the characteristics of AIEC pathobionts retrieved from ileal biopsies of CD patients enrolled in France (FR) and Hong Kong (HK). The prevalence of AIEC was similar in France (24.5%, 25/102) and Hong Kong (30.0%, 18/60) ( p = 0.44). No difference was observed between the two populations of AIEC regarding adhesion and invasion levels. When tested for antibiotic resistance, the proportion of AIEC strains resistant to ampicillin, piperacillin, tobramycin, and gentamicin was significantly higher in HK AIEC strains compared to French strains. AIEC strains from FR or HK population were both able to persist in the mice intestine (DSS-treated CEABAC10 mice model). Moreover, genomic analysis of 25 FR and 17 hK AIEC strains using next-generation sequencing revealed the co-existence of several virulence factors associated with enteric E. coli pathotypes, although no single virulence factor was significantly associated with either country of origin or AIEC status. In vitro , all AIEC strains (FR and HK) were sensitive to the EcoActive™ phage cocktail, suggesting that it could be a promising option to target AIEC in CD across the world.

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Comparison of Crohn’s disease-associated adherent-invasive Escherichia coli (AIEC) from France and Hong Kong: results from the Pacific study

Chevarin,

Xu,

Martin

et al. 2024

Gut Microbes

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Metatranscriptomics-based metabolic modeling of patient-specific urinary microbiome during infection

Josephs-Spaulding,

Rettig,

Zimmermann

et al. 2024

Preprint

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Urinary tract infections (UTIs) are a major health concern which incurs significant socioeconomic costs in addition to substantial antibiotic prescriptions, thereby accelerating the emergence of antibiotic resistance. In addressing the challenge of antibiotic-resistant UTIs, our approach harnesses patient-specific metabolic insights to hypothesize treatment strategies. By leveraging the distinct metabolic traits of pathogens, we aim to identify metabolic dependencies of pathogens and pave the way for more targeted therapeutic interventions. Combining patient-specific metatranscriptomic data with genome-scale metabolic modeling, we explored the metabolic aspects of UTIs from a systems biology perspective. We created tailored microbial community models, each mirroring the metabolic profiles of individual UTI patients' urinary microbiomes. Delving into patient-specific bacterial gene expressions and microbial interactions, we identify metabolic signatures and propose mechanisms for UTI pathology. We identified several patient-specific metabolites linked to infection that could inform therapeutic approaches. Our research underscores the potential of integrating metatranscriptomic data using systems biological approaches, offering insights into disease metabolic mechanisms and potential phenotypic manifestations. This contribution introduces a new method that could guide treatment options for antibiotic-resistant UTIs, aiming to lessen antibiotic use by combining the pathogens' unique metabolic traits.

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Antioxidant Role of Probiotics in Inflammation-Induced Colorectal Cancer

Hamamah,

Lobiuc,

Covasa

2024

IJMS

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Colorectal cancer (CRC) continues to be a significant contributor to global morbidity and mortality. Emerging evidence indicates that disturbances in gut microbial composition, the formation of reactive oxygen species (ROS), and the resulting inflammation can lead to DNA damage, driving the pathogenesis and progression of CRC. Notably, bacterial metabolites can either protect against or contribute to oxidative stress by modulating the activity of antioxidant enzymes and influencing signaling pathways that govern ROS-induced inflammation. Additionally, microbiota byproducts, when supplemented through probiotics, can affect tumor microenvironments to enhance treatment efficacy and selectively mediate the ROS-induced destruction of CRC cells. This review aims to discuss the mechanisms by which taxonomical shifts in gut microbiota and related metabolites such as short-chain fatty acids, secondary bile acids, and trimethylamine-N-oxide influence ROS concentrations to safeguard or promote the onset of inflammation-mediated CRC. Additionally, we focus on the role of probiotic species in modulating ROS-mediated signaling pathways that influence both oxidative status and inflammation, such as Nrf2-Keap1, NF-κB, and NLRP3 to mitigate carcinogenesis. Overall, a deeper understanding of the role of gut microbiota on oxidative stress may aid in delaying or preventing the onset of CRC and offer new avenues for adjunct, CRC-specific therapeutic interventions such as cancer immunotherapy.

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Molecular epidemiology, antibiotic resistance profile and frequency of integron 1 and 2 in adherent-invasive Escherichia coli isolates of colorectal cancer patients

Cited by 3 publications

References 65 publications

Comparison of Crohn’s disease-associated adherent-invasive Escherichia coli (AIEC) from France and Hong Kong: results from the Pacific study

Comparison of Crohn’s disease-associated adherent-invasive Escherichia coli (AIEC) from France and Hong Kong: results from the Pacific study

Metatranscriptomics-based metabolic modeling of patient-specific urinary microbiome during infection

Antioxidant Role of Probiotics in Inflammation-Induced Colorectal Cancer

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