Molecular Epidemiology: Application of Contemporary Techniques to the Typing of Microorganisms

Maslow, Joel N.; Mulligan, Maury E.; Arbeit, Robert D.

doi:10.1093/clinids/17.2.153

Cited by 379 publications

(277 citation statements)

References 29 publications

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“…If a typing method is to be judged on the criteria of typability, reproducibility and discriminatory power, as suggested by Maslow [12] then we found phagetyping was less satisfactory than either ribotyping or PFGE according to the first two criteria. Overall, approximately 22 % of all strains were non-typable by phage-typing.…”

Section: Discussionmentioning

confidence: 80%

Comparison of PFGE, ribotyping and phage-typing in the epidemiological analysis ofCampylobacter jejuniserotype HS2 infections

Gibson¹,

Fitzgerald²,

Owen³

1995

Epidemiol. Infect.

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SUMMARYIn this study we have evaluated the ability of three typing methods, pulsed field gel electrophoresis (PFGE), phage-typing and ribotyping, to discriminate not only between strains of differing serotypes but also between strains within a single serotype, heat stable serotype 2 (HS2). Forty-five isolates derived from cases of campylobacter enteritis occurring in the Cardiff area were examined. These included 18, mostly HS2, strains associated with an outbreak. The typing results for these and a further 39 epidemiologically unrelated strains of serotype HS2 were compared. This is the first report documenting the use of PFGE in an epidemiological investigation of Camnpylobacter jejuni in the UK. The results presented suggest that this technique is the most discriminatory of the three subtyping methods examined.

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Section: Discussionmentioning

confidence: 80%

Comparison of PFGE, ribotyping and phage-typing in the epidemiological analysis ofCampylobacter jejuniserotype HS2 infections

Gibson¹,

Fitzgerald²,

Owen³

1995

Epidemiol. Infect.

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“…The use of several molecular typing techniques for the effective detection of outbreaks of infection as well as identification of new and infectious clones of H. influenzae has been reported in recent years [9,10]. Among these, restriction fragment length polymorphism analysis with ribosomal RNA (rRNA) as the probe [11][12][13] has been used widely to differentiate isolates into ribotypes. Intra-specific genetic variation can also be detected by randomly amplified polymorphic DNA (RAPD) analysis.…”

Section: Introductionmentioning

confidence: 99%

Subtype distribution of Haemophilus influenzae isolates from North India

Sharma

Kaur

Ganguly

et al. 2002

Journal of Medical Microbiology

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A total of 120 Haemophilus influenzae isolates from blood, cerebrospinal fluid, sputum and throat swabs of patients and carriers in North India was characterised by biotyping, ribotyping and random amplification of polymorphic DNA (RAPD)-PCR. Of these, 77 isolates (64%) were serotype b; the other 43 (36%) were non-typable. Biotype I was the most predominant among the typable strains and biotype II among the non-typable strains. Ribotyping with restriction endonucleases HaeIII and EcoRI differentiated the isolates into three and six ribotypes, respectively. However, RAPD fingerprints generated by the application of arbitrary primers AP1 and AP2 provided a higher level of discrimination. RAPD typing revealed distinct polymorphism among the serologically typable isolates. This study is the first report that stratifies the subtypes of H. influenzae strains from India by molecular techniques.

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“…Multilocus enzyme electrophoresis (MEE), which has been used for genetic analysis, and for determining the population structure of various groups of bacteria, including mycobacteria [24, 25], has the advantage of measuring the proportional difference between the genomes of different isolates [26]. Pulsed field gel electrophoresis (PFGE) also has been shown to be useful for identification of specific strains [27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization ofMycobacterium aviumisolates recovered from humans and animals in Australia

Feizabadi

Robertson

Cousins³

et al. 1996

Epidemiol. Infect.

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SUMMARYGenetic relationships amongst 115 mainly Australian isolates of Mycobacterium avium were assessed using multilocus enzyme electrophoresis (MEE). The isolates were divided into 58 electrophoretic types (ETs), with a mean genetic diversity of 0-29. Isolates from humans were closely related to but distinct from those cultured from birds, whilst some porcine isolates belonged to the same ETs as certain human isolates. Pulsed field gel electrophoresis (PFGE) was used to differentiate related isolates, and those from birds and some from other animals, including pigs, were distinguished from the human isolates. The results of MEE and PFGE suggested that certain strains of M. avium may be transmitted between birds and pigs, but there was no clear evidence of transmission to humans. The serovar of the M. avium isolates was not obviously related to their ET assignment or their PFGE type.

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Molecular Epidemiology: Application of Contemporary Techniques to the Typing of Microorganisms

Cited by 379 publications

References 29 publications

Comparison of PFGE, ribotyping and phage-typing in the epidemiological analysis ofCampylobacter jejuniserotype HS2 infections

Comparison of PFGE, ribotyping and phage-typing in the epidemiological analysis ofCampylobacter jejuniserotype HS2 infections

Subtype distribution of Haemophilus influenzae isolates from North India

Genetic characterization ofMycobacterium aviumisolates recovered from humans and animals in Australia

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