Molecular epidemiology of adenovirus conjunctivitis in Rio de Janeiro, Brazil, between 2004 and 2007

Maranhão, Adriana Gonçalves; Soares, Caroline C.; Albuquerque, Maria Carolina M.; Santos, Norma

doi:10.1590/s0036-46652009000400010

Cited by 34 publications

(44 citation statements)

References 22 publications

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“…Other non-enteric HAdVs, such as species B, C, and D that are known to cause respiratory and ocular diseases 23,29,30 , were also detected in our study. This observation may be explained by the ability all species have of replicating in the intestine and being excreted in the feces 26,31 .…”

Section: Discussionsupporting

confidence: 64%

Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

Costa

Siqueira

Portal

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Acute gastroenteritis (AGE) is one of the most common causes of morbidity and mortality, especially among children from developing countries. Human adenovirus (HAdV) and sapovirus (SaV) are among the agents that cause AGE. The present study aimed to detect and genotype HAdV and SaV in 172 fecal samples from children with AGE, collected during a surveillance study carried out in a low-income community in Belém, Pará, between 1990 and 1992. Methods: HAdV was detected by nested PCR, using primers Hex1deg/Hex2deg and NeHex3deg/NeHex4deg. SaV was assayed by reverse transcription PCR (RT-PCR), nested PCR, and quantitative PCR. The nucleotide sequence was determined by direct cycle sequencing. Results: Overall, 43% (74/172) of samples were positive for HAdV, of which 70.3% (52/74) were sequenced and classified as belonging to five different species, mostly A and F. For SaV, positivity was 5.2% (9/172) and genotypes GI.1, GI.7, GII.1, and GV.2 were detected. Conclusions: The present results reinforce the need for further studies to obtain epidemiological data about the circulation of these viruses in Brazil, especially in the Amazon Region, where data from the early 1990's are scarce. Furthermore, the study describes for the first time the detection of SaV genotypes GI.7 and GV.2 in Brazil, showing that these types circulated in the region more than 25 years ago.

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Section: Discussionsupporting

confidence: 64%

Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

Costa

Siqueira

Portal

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…, 56 cause epidemic keratoconjunctivitis and serotypes 3, 4 and 7 cause pharyngoconjunctival fever (5,7,8). Outbreaks of adenoviral infections are common (9).…”

Section: In This Study It Was Aimed To Investigate Adenoviruses Frommentioning

confidence: 99%

“…by the rate of 15-70 % worldwide (5). Adenoviral eye infections are clinically seen as follicular conjunctivitis, pharyngoconjunctival fever and epidemic keratoconjunctivitis (6).…”

mentioning

confidence: 99%

Investigation of Adenoviruses in Patients with Conjunctivitis by Various Methods

Kiraz

Gökahmetoğlu

Özüberk

et al. 2013

EUR J BASIC MED SCI

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Adenoviruses are the most common causative agents of epidemics of community acquired and nosocomial conjunctivitis. Adenoviruses are resistant to physical and chemical factors and they can spread easily. Due to these factors, rapid and accurate diagnosis is very important in adenoviral eye infections. In this study, it was aimed to investigate adenoviruses from conjunctival swab samples of 50 patients suspected of adenoviral conjunctivitis by two different cell culture and PCR methods. Conjunctival swab samples from patients suspected of adenoviral conjunctivitis were taken between March 2012-December -11, 15, 16, 17, 19, 20, 22 cause conjunctivitis, serotypes 2, 3, 4, 5, 7, 8, 10, 11, 19, 21, 22, 29, 34, 37, 53, 54, 56 cause epidemic keratoconjunctivitis and serotypes 3, 4 and 7 cause pharyngoconjunctival fever (5,7,8). Outbreaks of adenoviral infections are common (9). Due to remaining infectious at room temperature for weeks and high level of resistance to environmental factors, transmission of adenovirus may occur easily (9,10). As can be transmitted from person to person, transmission may occur through water, objects and medical devices. Therefore nosocomial adenovirus infections can be seen (10).Adenoviral infections are mainly diagnosed by direct methods such as cell culture, nucleic acid detection and antigen detection (10,11). Indirect diagnosis based on the serological methods are mainly preferred in epidemiological researches instead of diagnosing adenoviral infections because of their low sensitivity rates (11). Rapid diagnose in adenoviral eye infections, especially in outbreaks of nosocomial conjunctivitis is very important and essential for confirmation of clinical diagnosis, limiting the spread of infection, implementation of appropriate protective measures and prevention of inappropriate treatment (12). Promptly isolation of infected individuals is critically important for control of outbreaks in both community acquired and nosocomial outbreaks (13). In this study, it was aimed to investigate adenovirus from conjunctival swab samples of patients suspected of adenoviral conjunctivitis by cell culture and PCR methods. MATERIALS AND METHODSThis study was performed in Virology Laboratory, with approval of Local Ethics Committee approval (06/05/2010) and supported by Scientific Research Projects Department (TSU-11-3645 code number). Total of 50 patients admitted to Ophthalmology Clinic whom suspected of adenoviral conjunctivitis between March 2012-December 2012 were included in this study. Two sets of conjunctival swab samples using plastic handle with a Dacron swab were taken from each patient. All samples were hold in viral transport medium (Vircell, Spain) in -70 °C until the day of study. One of the viral transport medium was used in

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“…Conjunctivitis caused by serotypes 8, 19 and 37 is more severe and can spread faster than the one caused by other serotypes [4][5][6][7]. The propensity of adenoviral conjunctivitis to cause outbreaks and visual impairment makes identification of adenovirus essential so that preventive measures are initiated as early as possible [8].…”

Section: Introductionmentioning

confidence: 99%

“…The propensity of adenoviral conjunctivitis to cause outbreaks and visual impairment makes identification of adenovirus essential so that preventive measures are initiated as early as possible [8]. Serotype identification of adenovirus is critical for epidemiological surveillance, detection of new strains and understanding the pathogenicity of the virus [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Investigational approach to adenoviral conjunctivitis: comparison of three diagnostic tests using a Bayesian latent class model

Sundaramurthy¹,

Dhodapkar

Kaliaperumal

et al. 2018

J Infect Dev Ctries

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Introduction: Highly contagious adenoviral conjunctivitis represents 15-70% of all conjunctivitis worldwide. Human adenovirus (hAdV) serotypes 3,4,7,8,19 and 37 contributes to 89% of all adenoviral conjunctivitis. Accurate and rapid diagnosis of adenoviral infections at serotype level could prevent misdiagnosis, spread of disease, unnecessary antibiotic use and increased treatment costs. Methodology: Sixty-two suspected viral conjunctivitis cases were recruited from November2013-January2015. Swabs collected from inferior palpebral conjunctiva and processed for viral culture (Hep2 cell line), immunofluorescence assay (IFA) and polymerase chain reaction (PCR) (targeting hexon gene). Serotype 3,4,7,8,19 and 37 identification was carried out with an optimized multiplex-PCR (based on hypervariable region of hexon gene) and confirmed by sequence analysis. Bayesian Latent Class Model (LCM) analysis was used to compare sensitivity and specificity of three tests. Results: Adenovirus was detected in 54.8% (34/62) of cases by combination of all three methods. Culture was positive in 23/34 cases (67.6%). PCR and IFA detected adenovirus in 24 (70.5%) and 21 (61.7%) cases respectively. LCM analysis revealed, sensitivity and specificity of PCR, Culture and IFA was 77.8% and 92.4%; 72.2% and 90.8%; 67.6% and 92.9% respectively. Serotyping by multiplex-PCR showed, two cases each were hAdV3 and hAdV4, 18 hAdV8 and two remained unidentified. Results of Multiplex-PCR and sequence analysis showed 100% concordance Conclusion: LCM analysis revealed, PCR is the most appropriate method for identification. Multiplex-PCR is a simple and rapid method (serotypes identification within two days); owing its short turnaround time and accuracy, it can be used as a diagnostic tool for surveillance of adenoviral keratoconjunctivitis.

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Molecular epidemiology of adenovirus conjunctivitis in Rio de Janeiro, Brazil, between 2004 and 2007

Cited by 34 publications

References 22 publications

Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

Investigation of Adenoviruses in Patients with Conjunctivitis by Various Methods

Investigational approach to adenoviral conjunctivitis: comparison of three diagnostic tests using a Bayesian latent class model

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