Molecular epidemiology of adenoviruses isolated in Iceland during 1988–1990

Adenoviruses isolated in the Stockholm area during 1987?1992: restriction endonuclease analysis and molecular epidemiology

“…In Stockholm, Adl was found as often as Ad2, whereas Ad5 was only rarely encountered. Among the Adl strains, the DNA-variant D10 prevailed, in contrast to adenorviruses isolated in Iceland [22] with only a single-isolate of this DNA-variant. Nonetheless, D10 was frequently found in Hannover as well as worldwide.…”

“…The DNA-variants D1, D3, and D10 co-circulated to about the same extent during the first 12 months, thereafter D10 predominated. Noteworthy, D1 was isolated in Stockholm but absent in a concomitant Icelandic epidemic [22]. An interesting observation of unclear importance, was the predominance of ocular origin of the D1 isolates (5/8) as compared to D3 and D10 both together (2/32).…”

“…Figure 1 (a and b). The Kpn I pattern x of Ad I and the x patterns of Barn HI and BstEII of Ad5 were originally found in Iceland [22] Subgenus E Ad4 is the sole serotype within subgenus E. Strains of b o t h g e n o m i c clusters of Ad4 ( p r o t o t y p e a n d "a", respectively, a c c o r d i n g to Li a n d Wadell [28]) were identified (Fig. 5).…”

“…The prototype (D1) of each serotype has the code: 1 l 11111. The novel RE-patterns for respective enzyme have been designated x or y (the y-pattern of Hin dIII of Ad2 because an x-pattern already had been described [22]. The novel DNA-variants of respective serotype have preliminarly been named Stol 4.…”

“…Adenovirus DNA was extracted, cleaved by restriction endonucleases, and separated in agarose gels as previously described [22].…”