Molecular epidemiology of antimicrobial resistance among Salmonella enterica serovar Infantis from poultry in Kagoshima, Japan

Shahada, Francis; Chuma, Takehisa; Tobata, Takayuki; Okamoto, Karoku; Sueyoshi, Masuo; Takase, Kozo

doi:10.1016/j.ijantimicag.2006.07.003

Cited by 76 publications

(65 citation statements)

References 26 publications

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“…All of the kanamycinresistant isolates contained the aphA1-1AB gene, similar to previous reports [13,25]. However, it was detected at a low rate from S. Infantis in Japan [31]. In this study, the resistance genes were found at high rates and were widespread in different serovars from the resistant isolates, indicating that these genes play an important role in prevalence of antimicrobial resistance among the Salmonella isolates from retail meats in Vietnam.…”

Section: Discussionsupporting

confidence: 89%

“…Detection of resistance genes: DNA templates used for PCR were prepared by boiling bacterial cultures [31]. The following genes implicated with antimicrobial resistance were detected by PCR amplification: bla PSE-1 , bla OXA-1 and bla TEM encoding β-lactam resistance; aadA1, aadA2, aac (3)-IV, aphA-1AB and Kn encoding aminoglycoside resistance; catA1, cmlA1 and floR encoding chloramphenicol resistance; sul1 encoding sulphonamide resistance; tetA, tetB and tetG encoding tetracycline resistance; and dfrA1 and dfrA12 encoding trimethoprim resistance.…”

Section: Methodsmentioning

confidence: 99%

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Antimicrobial Resistance of <i>Salmonella</i> Serovars Isolated from Beef at Retail Markets in the North Vietnam

et al. 2012

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ABSTRACT. Approximately 39.9% (63/158) of beef samples collected from retail markets in Hanoi from January to June 2009 were Salmonella-positive. Nine Salmonella serovars, Anatum (28.6%), Rissen (25.4%), Weltevreden (12.7%), Typhimurium (7.9%), Derby (7.9%), Lexington (7.9%), Dublin (4.6%), Newport (3.2%) and London (1.8%), were identified. Thirty-seven (58.7%) of the 63 Salmonella isolates were resistant to at least one antimicrobial tested, of which 29 (46%) isolates showed multidrug resistance (MDR). The isolates were commonly resistant to tetracycline (46.0%), sulphonamide (39.7%), ampicilline (31.7%), streptomycin (30.2%), trimethoprim (28.6%), kanamycin (28.6%) and chloramphenicol (22.2%). Fourteen (bla TEM , bla aadA1, aadA2, sul1, tetA, tetB, tetG, cmlA1, floR, dfrA1, dfrA12, aac (3)-IV and aphA1-1AB) out of 22 antimicrobial resistance genes were detected by PCR from the resistant isolates. The catA1, Kn, bla PSE-1 genes and plasmid-mediated quinolones resistance (PMQR) genes such as qnrA, qnrB, qnrS, qepA and acc (6 ' )-ib-cr were not detected. Mutations in the gyrA gene leading to the amino acid changes Ser83Phe and/or Asp87Asn were found in 6 out of the 11 quinolone-resistant isolates. The data revealed that multidrug resistant Salmonella strains were widely distributed in north Vietnam via the food chain and might contain multiple genes specifying identical resistant phenotypes. Thus, continuous studies are necessary to clarify the mechanisms of MDR in Salmonella and its spread in the livestock market.

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Antimicrobial Resistance of <i>Salmonella</i> Serovars Isolated from Beef at Retail Markets in the North Vietnam

et al. 2012

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“…Highly similar antibiotic resistance characteristic of the strains were indicating the clonal dissemination of these clusters. The emergence of multidrug-resistant clones in S. Infantis in broilers and humans was reported in several countries (Marimon et al 2004;Shahada et al 2006;Asai et al 2007). All S. Infantis strains presented class I integrons with the variable regions of 1.0 kb and 1.2 kb.…”

Section: Resultsmentioning

confidence: 96%

Phenotypic and genetic characterization of multidrug-resistant Salmonella Infantis strains isolated from broiler chicken meats in Turkey

Abbasoglu

Akçelik

2011

Biologia

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Twenty Salmonella Infantis strains resistant against kanamycin, tetracycline, neomycin, spectinomycin, sulphonamide, nalidixic acid and trimethoprim were selected for this study out of 103 Salmonella strains isolated from broiler samples collected from several markets in the Bolu and Ankara regions of Turkey. The resistance genes aadA1, aphA1, sul1, tet(A), dfrA5/dfrA14 and gyrA were determined for these multidrug-resistant S. Infantis strains. S. Infantis strains contained a mega plasmid with the molecular size of 206 kb. The strains were divided into three groups according to the pulsed field gel electrophoresis patterns of XbaI digested chromosomal DNA. A Ser83→Tyr83 point mutation was found in the gyrA gene of all quinolone-resistant isolates. Filter mating experiments showed that 206 kb plasmid transferred nalidixic acid resistance associated with class I integrons.

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“…It is well established on poultry farms with a tendency of clonal spread of the multidrug resistance phenotype. Clonal spread of Salmonella Infantis in poultry and poultry meat was reported in Japan (Shahada et al, 2006), Hungary (Nógrády et al, 2007), Israel (Gal-Mor et al, 2010), Italy (Dionisi et al, 2011), Germany (Hauser et al, 2012), Serbia (Rašeta et al, 2014;Velhner et al, 2014) but also in humans in Argentina (Merino et al, 2003) and Brazil (Fonseca et al, 2006). All these clonal strains were resistant to three or more antimicrobials except for Serbia, where the predominant resistance phenotype was nalidixic acid (NAL) / tetracycline (TET), while an approximate 30% of the isolates was showing resistance to ciprofloxacin (CIP), with the minimal inhibitory concentration (MIC) of > 1mg/L (Velhner et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Salmonella spp. in poultry: a constant challenge and new insights

Velhner

Milanov

Kozoderović

2018

J Hellenic Vet Med Soc

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ABSTRACT. The knowledge about virulence mechanisms, resistance to antimicrobial agents and the biofilm formation ability of Salmonella spp. in poultry industry has been expanded over the years. However, in spite of the research efforts and significant investments to improve management systems in poultry industry, it has become evident that none of the methods applied in all stages of food production chain are 100% effective in eliminating Salmonella spp. Different serovars are manifesting different mechanisms of invasiveness which depend on their ability to invade lower zones of the lamina propria, their ability to gain accesses to parenchymatous organs and survive in macrophages. The ubiquitous nature of Salmonella spp. due to their adaptation to animal and plant hosts, as well as their survival in hostile environments and their enhanced capacity to produce biofilms, contribute to a long lasting contamination of the environment, feed and animals. The emergency and spread of antimicrobial resistances in Salmonella spp. raise additional concerns.

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Molecular epidemiology of antimicrobial resistance among Salmonella enterica serovar Infantis from poultry in Kagoshima, Japan

Cited by 76 publications

References 26 publications

Antimicrobial Resistance of <i>Salmonella</i> Serovars Isolated from Beef at Retail Markets in the North Vietnam

Antimicrobial Resistance of <i>Salmonella</i> Serovars Isolated from Beef at Retail Markets in the North Vietnam

Phenotypic and genetic characterization of multidrug-resistant Salmonella Infantis strains isolated from broiler chicken meats in Turkey

Salmonella spp. in poultry: a constant challenge and new insights

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