Molecular Epidemiology of Bovine Enteroviruses and Genome Characterization of Two Novel Bovine Enterovirus Strains in Guangxi, China

Luo, Yuhang; Liu, Huanghao; Zou, Yanlin; Qiao, Chengpeng; Su, Yaquan; Zhu, Xinyue; Zhang, Guangxin; Huang, Wenfei; Qin, Yifeng; Yan, Ping; Huang, Weijian

doi:10.1128/spectrum.03785-22

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…BEV can easily co-infect with other viruses that cause pathogenic infections in cattle, resulting in significant economic losses in the cattle industry (Luo et al, 2023). Although studies on infections caused by enteroviruses have been conducted in various animal species worldwide and in Türkiye, they have mainly focused on cattle.…”

Section: Discussionmentioning

confidence: 99%

“…Despite BEV infects mammals orally, this pathogen is unaffected by the gastrointestinal tract, travels from the digestive tract to the lymph nodes, and elicits an antibody response. Neutralizing antibodies specific to BEV have been detected in many species, including humans, cattle, sheep, goats, dogs, horses, and monkeys (Luo et al, 2023). Although the disease usually presents as a subclinical infection with mild symptoms, fatal cases with low morbidity have rarely been reported (Blas-Machado et al, 2007).…”

Section: Disease Virus Equine Rhinitis a Virus) Cardiovirus (Cardiovi...mentioning

confidence: 99%

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Serological Investigation of Bovine Enterovirus in Calves in Konya Province

Palancı,

Avcı,

Dik

et al. 2024

Erciyes Üniversitesi Veteriner Fakültesi Dergisi

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Bovine enterovirus (BEV) infection is a common viral disease in cattle. Although the infection is often subclinical, it is among the possible causes of gastroenteritis. BEV infections have also been associated with respiratory and reproductive system disorders and signs of diarrhea. BEV is divided into two serotypes, enterovirus serotype E (EV-E) and enterovirus serotype F (EV-F). This study aims to determine the seroprevalence of EV-E in calves in Konya province. For this purpose, 504 BEV unvaccinated calf blood serum samples from the Selcuk University Veterinary Faculty Virology Department laboratory were used. A serum neutralization test (SNT) was used to determine EV-E seroprevalence. 342 (67.85%) samples were found to be seropositive, and 162 (32.14%) were seronegative. In addition, as the serum neutralization 50 (SN50) antibody titers of seropositive animals were examined, the titers determined as 1/10, 1/15 and 1/20. Among the results we obtained, the highest antibody titers were defined as 1/80 and 1/120; the total number of animals with these values was In conclusion, bovine enteroviruses are an infection of importance for cattle breeding. Therefore, necessary precautions must be taken to protection infection. It is thought that the results of the present study will provide important data for future studies.

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Section: Discussionmentioning

confidence: 99%

Section: Disease Virus Equine Rhinitis a Virus) Cardiovirus (Cardiovi...mentioning

confidence: 99%

Serological Investigation of Bovine Enterovirus in Calves in Konya Province

Palancı,

Avcı,

Dik

et al. 2024

Erciyes Üniversitesi Veteriner Fakültesi Dergisi

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“…Recombination rates in Enterovirus B are high, and a vast number of recombinant forms have been identified in this species ( 14 – 16 ). Recently, Luo et al reported that BEV strains (GXNN2204 and GXGL2215) were, respectively, obtained from the genomic recombination of EV-E4 with EV-F3, and EV-E2 to EV-E4 ( 17 ). Recombination events in EVs are almost exclusively detected at the edges of the structural P1 region and within the nonstructural 5′UTR, P2, or P3 regions ( 14 , 18 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and recombination of bovine enterovirus strains in China

Chang,

Zhang,

Cui

et al. 2024

Microbiol Spectr

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Bovine enterovirus (BEV) consisting of enterovirus species E (EV-E) and F (EV-F) is the causative agent associated with respiratory and gastrointestinal diseases in cattle. Here, we reported the characterization, genetic diversity, and recombination of novel BEV strains isolated from the major cattle-raising regions in China during 2012–2018. Twenty-seven BEV strains were successfully isolated and characterized. Molecular characterization demonstrated that the majority of these novel BEV strains (24/27) were EV-E, while only few strains (3/27) were EV-F. Sequence analysis revealed the diversity of the circulating BEV strains such as species and subtypes where different species or subtype coinfections were detected in the same regions and even in the same cattle herds. For the EV-E, two novel subtypes, designated as EV-E6 and EV-E7, were revealed in addition to the currently reported EV-E1–EV-E5. Comparative genomic analysis revealed the intraspecies and interspecies genetic exchanges among BEV isolates. The representative strain HeN-B62 was probably from AN12 (EV-F7) and PS-87-Belfast (EV-F3) strains. The interspecies recombination between EV-E and EV-F was also discovered, where the EV-F7-AN12 might be from EV-E5 and EV-F1, and EV-E5-MexKSU/5 may be recombined from EV-F7 and EV-E1. The aforementioned results revealed the genetic diversity and recombination of novel BEV strains and unveiled the different BEV species or subtype infections in the same cattle herd, which will broaden the understanding of enterovirus genetic diversity, recombination, pathogenesis, and prevention of disease outbreaks. IMPORTANCE Bovine enterovirus (BEV) infection is an emerging disease in China that is characterized by digestive, respiratory, and reproductive disorders. In this study, we first reported two novel EV-E subtypes detected in cattle herds in China, unveiled the coinfection of two enterovirus species (EV-E/EV-F) and different subtypes (EV-E2/EV-E7, EV-E1/EV-E7, and EV-E3/EV-E6) in the same cattle herds, and revealed the enterovirus genetic exchange in intraspecies and interspecies recombination. These results provide an important update of enterovirus prevalence and epidemiological aspects and contribute to a better understanding of enterovirus genetic diversity, evolution, and pathogenesis.

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