Molecular epidemiology of Brucella abortus isolated from cattle in Brazil, 2009–2013

Oliveira, Mayra Silva; Dorneles, Elaine Maria Seles; Soares, Paulo Martins; Fonseca, Antônio Augusto; Orzil, Lívia de Lima; Souza, Patrícia Gomes de; Lage, Andrey Pereira

doi:10.1016/j.actatropica.2016.10.023

Cited by 14 publications

(7 citation statements)

References 33 publications

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“…The two strains of B. abortus isolated from cervical bursitis exhibited different biochemical and molecular tests than B. abortus biovar 1, the most common strain causing bovine brucellosis in Brazilian territory (MINHARRO et al, 2013;OLIVEIRA et al, 2017). All the tests used allowed us to state without doubt that the two isolates are in fact B. abortus biovar 4, being the first isolated strains of this biovar in Brazil (MINHARRO et al, 2013).…”

Section: Discussionmentioning

confidence: 97%

“…Although, important for the diagnosis of the disease, phenotypic typing methods generally have less discriminatory power compared with genotypic methods and; therefore, make it difficult to track outbreaks and control the spread of the disease (MINHARRO et al, 2013). In this context, the intraspecific characterization of B. abortus at biovar and molecular levels are fundamental for a better understanding of the disease epidemiology, for formulation of effective strategies of infection control and eradication and for solving outbreaks (DORNELES et al, 2014;OLIVEIRA et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Among the molecular typing methodologies commonly used for Brucella spp., Multiple Locus Variable Number of Tandem Repeat (VNTR) Analysis (MLVA) and Multiple Locus Sequence Typing (MLST) are well-adapted techniques that have proved to be valuable tools in source tracking and in the intraspecific classification of Brucella spp. isolates (OLIVEIRA et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil

et al. 2023

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The aim of the present study was to characterize (phenotypically and genotypically) two strains of Brucella abortus identified as belonging to biovar 4 isolated from cattle in Brazil. The strains were isolated from cervical bursitis from cattle in the states of Pará and Rio Grande do Sul, respectively. In the phenotypic identification, the isolates were positive in CO2 requirement, produced H2S, were resistant to basic fuchsin (20 µg / mL) and sensitive to thionin (20 µg / mL and 40 µg / mL) and presented M surface antigen, but A surface antigen is absent. The isolates were positive in the PCR for the bcsp31 gene (genus-specific) and in the AMOS-enhanced PCR, both isolates showed a band profile consistent with B. abortus biovar 1, 2 or 4. Moreover, both isolates also showed restriction patterns identical to the reference strain when tested by the omp2b PCR-RFLP. In genotyping using Multiple Locus Variable Number of Tandem Repeat (VNTR) Analysis - MLVA (MLVA16), the isolates showed differences in several loci (Bruce42, Bruce19, Bruce04, Bruce16 and Bruce30); by Multiple Locus Sequence Typing (MLST), they also exhibited differences in sequence type (ST), strain 16/02 ST1 (2-1-1-2-1-3-1-1-1) and strain 128/11 ST (22-1-1 -8-9-3-1-1-1). The extensive typing of B. abortus strains isolated from cattle in Brazil using different approaches confirmed the occurrence of rare B. abortus biovar 4 in the country.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil

et al. 2023

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“…Some countries (such as Australia, Germany, Finland and the UK) have controlled the disease, however it still remains prevalent in many regions such as the Middle East, Latin America, and central and eastern Asia 22 . Syria is among the top ten countries with the highest incidence 23 .…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of brucellosis antibodies and associated risk factors among the hospitalized patient, Aleppo, Syria: a hospital-based cross-sectional study

Abdulrazzak,

Alshaghel,

Anadani

et al. 2024

Annals of Medicine &Amp; Surgery

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Introduction and Importance: Brucellosis is one of the most common infectious diseases in the world, especially in developing countries. Recent reports show that Syria is among the top ten countries where brucellosis is most prevalent. The purpose of this study is to estimate the seroprevalence of brucellosis anti-bodies among the hospitalized patients, in one of the largest hospitals in northern Syria. Materials and Methods: A cross-sectional study was conducted among the hospitalized patients. We used a questionnaire to collect sociodemographic and brucellosis-related data from the patients. We also collected blood samples from these patients to be screened for brucellosis antibodies using Wright Coombs Agglutination and 2-mercaptoethanol tests, during the period from November 2021 and March 2022. Results: Among the 776 patients who were recruited in the study, the seroprevalence of brucellosis antibodies was 13.1% (n=776). The highest prevalence was among the female gender (16.7%, n=298), middle aged group 12-40 years (24.1%, n=116), and patients with history of brucellosis (30.1%, n=53). Among the positive samples, the findings of 2-mercaptoethanol tests show that (14.7%, n=102) were positive (presence of IgG Antibodies), and (75.5%, n=102) were negative. Conclusion: This study is the first to describe the epidemiology of brucellosis in northern Syria. It clearly shows high rates of positivity, which reflects immense challenges facing the public health sector in Syria. The best next step in light of this crisis is to raise awareness among population about brucellosis and its risk factor.

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“…Kohgiluyeh and Boyer Ahmad province, in terms of geographical location and its high share in livestock breeding, can be among the cases of exposure to brucellosis. The brucellosis fever can be transmitted through conjunctiva, scratches, excreta, contaminated tissues, and through the respiratory system to humans [13]. Human transmission to humans is rare (14).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of brucellosis in Kohgiluyeh and Boyer Ahmad Province in 2017

et al. 2018

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Introduction: Brucellosis is a common infectious disease between humans and livestock, which can cause severe economic and health damage to the community. Considering the importance of this study, the aim of this study was to determine the prevalence of brucellosis in Kohgiluyeh and Boyer Ahmad Province in 2017. Materials and Methods: This retrospective cohort study was conducted to determine the rate and causes of brucellosis in Kohgiluyeh and Boyer Ahmad province in 1396. Sampling was done by census. Information about 300 patients with brucellosis, collected from April to July 1996, was collected. In order to collect data from a researcher-made list, data collection for brucellosis patients was used. Descriptive statistics and SPSS 21 series software were used to analyze the data. Results: 17 cases had negative test. The highest number of occurrences in 2016 was 143 cases and the highest occurrence was 171 in the spring. Boyer Ahmad city has the highest rate with 107 cases and Bahmei city with the least amount of brucellosis in 9 cases. 360 people were from rural population. The group of livestock breeders with 147 cases was the most affected and the military group with the least one was the least. Conclusion: By planning properly and timely, you can prevent the disease, complications and dangers of brucellosis.

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Molecular epidemiology of Brucella abortus isolated from cattle in Brazil, 2009–2013

Cited by 14 publications

References 33 publications

Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil

Phenotypic and genotypic characterization of Brucella abortus biovar 4 isolates from cattle in Brazil

Seroprevalence of brucellosis antibodies and associated risk factors among the hospitalized patient, Aleppo, Syria: a hospital-based cross-sectional study

Prevalence of brucellosis in Kohgiluyeh and Boyer Ahmad Province in 2017

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