Molecular epidemiology of carbapenem resistant Enterobacteriaceae in Valle d’Aosta region, Italy, shows the emergence of KPC-2 producing Klebsiella pneumoniae clonal complex 101 (ST101 and ST1789)

Franco, Mariateresa Del; Paone, Laura; Novati, Roberto; Giacomazzi, Claudio; Bagattini, Maria; Galotto, Chiara; Montanera, Pier Giorgio; Triassi, Maria; Zarrilli, Raffaele

doi:10.1186/s12866-015-0597-z

Cited by 65 publications

(67 citation statements)

References 43 publications

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“…In our study we were for the first time able to detect this Balkan NDM-1 outside a medical setting [15,59–62]. The detected NDM-1 harboring Klebsiella pneumoniae ST101 is also associated with carbapenem resistant Klebsiella in the Mediterranean region, but this resistance is mediated via KPC-2 and OXA-48 [50,63]. …”

Section: Discussionmentioning

confidence: 99%

Enterobacteriaceae Isolated from the River Danube: Antibiotic Resistances, with a Focus on the Presence of ESBL and Carbapenemases

et al. 2016

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In a clinical setting it seems to be normal these days that a relevant proportion or even the majority of different bacterial species has already one or more acquired antibiotic resistances. Unfortunately, the overuse of antibiotics for livestock breeding and medicine has also altered the wild-type resistance profiles of many bacterial species in different environmental settings. As a matter of fact, getting in contact with resistant bacteria is no longer restricted to hospitals. Beside food and food production, the aquatic environment might also play an important role as reservoir and carrier. The aim of this study was the assessment of the resistance patterns of Escherichia coli and Klebsiella spp. out of surface water without prior enrichment and under non-selective culture conditions (for antibiotic resistance). In addition, the presence of clinically important extended spectrum beta lactamase (ESBL) and carbapenmase harboring Enterobacteriaceae should be investigated. During Joint Danube Survey 3 (2013), water samples were taken over the total course of the River Danube. Resistance testing was performed for 21 different antibiotics. Samples were additionally screened for ESBL or carbapenmase harboring Enterobacteriaceae. 39% of all isolated Escherichia coli and 15% of all Klebsiella spp. from the river Danube had at least one acquired resistance. Resistance was found against all tested antibiotics except tigecycline. Taking a look on the whole stretch of the River Danube the proportion of multiresistances did not differ significantly. In total, 35 ESBL harboring Enterobacteriaceae, 17 Escherichia coli, 13 Klebsiella pneumoniae and five Enterobacter spp. were isolated. One Klebsiella pneumoniae harboring NMD-1 carbapenmases and two Enterobacteriaceae with KPC-2 could be identified. Human generated antibiotic resistance is very common in E. coli and Klebsiella spp. in the River Danube. Even isolates with resistance patterns normally associated with intensive care units are present.

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Section: Discussionmentioning

confidence: 99%

Enterobacteriaceae Isolated from the River Danube: Antibiotic Resistances, with a Focus on the Presence of ESBL and Carbapenemases

et al. 2016

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“…A retroactive study on colistin-resistant Enterobacteriaceae isolates obtained from January 2013 to November 2015 at a hospital in China found two K. pneumoniae harboring mcr-1 and bla NDM-5 [Du 2016]. Four bla KPC-2 producing K. pneumoniae ST101 from a cohort of nosocomial CRE obtained in Italy from November 2013 to August 2014 had colistin resistance (MIC 16–125 μg/ml) through an unidentified mechanism [Del Franco 2015]. …”

Section: Contribution Of Carbapenemases To Mdr/xdr Enterobacteriaceaementioning

confidence: 99%

The rapid spread of carbapenem-resistant Enterobacteriaceae

Potter

D’Souza

Dantas

2016

Drug Resistance Updates

335

211

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Carbapenems, our one-time silver bullet for multidrug resistant bacterial infections, are now threatened by widespread dissemination of carbapenem-resistant Enterobacteriaceae (CRE). Successful expansion of Enterobacteriaceae clonal groups and frequent horizontal gene transfer of carbapenemase expressing plasmids are causing increasing carbapenem resistance. Recent advances in genetic and phenotypic detection facilitate global surveillance of CRE diversity and prevalence. In particular, whole genome sequencing enabled efficient tracking, annotation, and study of genetic elements colocalized with carbapenemase genes on chromosomes and on plasmids. Improved characterization helps detail the co-occurrence of other antibiotic resistance genes in CRE isolates and helps identify pan-drug resistance mechanisms. The novel β-lactamase inhibitor, avibactam, combined with ceftazidime or aztreonam, is a promising CRE treatment compared to current colistin or tigecycline regimens. To halt increasing CRE-associated morbidity and mortality, we must continue quality, cooperative monitoring and urgently investigate novel treatments.

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“…bla has been commonly associated with ST101 worldwide and, according to the results of an 11-year (2001 to 2011) molecular epidemiologic study of bla in Europe and North Africa, ST101 is the most frequently observed sequence type (13). Among the carbapenem-resistant K. pneumoniae STs identified in this study, the emergence of colistin resistance had been already reported in KPC-2-producing ST101 (14) and bla KPC-2 -and bla CTX-M -producing ST307 (15,16). Carbapenem-and colistin-resistant isolates of ST437 have been reported previously (17).…”

mentioning

confidence: 94%

Molecular Epidemiology of Colistin-Resistant, Carbapenemase-Producing Klebsiella pneumoniae in Serbia from 2013 to 2016

Novović

Trudić

Brkić

et al. 2017

Antimicrob Agents Chemother

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Twenty-seven colistin-resistant, carbapenemase-producing Klebsiella pneumoniae isolates were identified from hospitals in Serbia. All isolates were bla CTX-M-15 positive; ST101, ST888, ST437, ST336, and ST307 were bla OXA-48 positive; and ST340 was bla NDM-1 positive. ST307 had an insertion, and ST336 had a premature stop codon in the mgrB gene. Amino acid substitutions were detected in PmrAB of isolates ST101, ST888, ST336, and ST307. The mcr-1 and mcr-2 were not detected. An increase in phoP, phoQ, and pmrK gene transcription was detected for all sequence types.

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Molecular epidemiology of carbapenem resistant Enterobacteriaceae in Valle d’Aosta region, Italy, shows the emergence of KPC-2 producing Klebsiella pneumoniae clonal complex 101 (ST101 and ST1789)

Cited by 65 publications

References 43 publications

Enterobacteriaceae Isolated from the River Danube: Antibiotic Resistances, with a Focus on the Presence of ESBL and Carbapenemases

Enterobacteriaceae Isolated from the River Danube: Antibiotic Resistances, with a Focus on the Presence of ESBL and Carbapenemases

The rapid spread of carbapenem-resistant Enterobacteriaceae

Molecular Epidemiology of Colistin-Resistant, Carbapenemase-Producing Klebsiella pneumoniae in Serbia from 2013 to 2016

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