Molecular epidemiology of chicken anaemia virus in sick chickens in China from 2014 to 2015

Yao, Shuai; Tuo, Tianbei; Gao, Xiang; Han, Chunyan; Yan, Nana; Liu, Aijing; Gao, Honglei; Gao, Yulong; Cui, Hongyu; Liu, Changjun; Zhang, Yanping; Qi, Xiaole; Hussain, Altaf; Wang, Yongqiang; Wang, Xiaomei

doi:10.1371/journal.pone.0210696

Cited by 23 publications

(18 citation statements)

References 56 publications

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“…Until 2011, chicken anemia virus (CAV) was the only Gyrovirus member identified. From that moment, several novel gyroviruses were characterized in humans and birds [52,53,54]. Partial genome of CAV was found in the present study.…”

Section: Discussionmentioning

confidence: 50%

Faecal Virome Analysis of Wild Animals from Brazil

Duarte

Silva

Brito

et al. 2019

Viruses

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The Brazilian Cerrado fauna shows very wide diversity and can be a potential viral reservoir. Therefore, the animal’s susceptibility to some virus can serve as early warning signs of potential human virus diseases. Moreover, the wild animal virome of this biome is unknown. Based on this scenario, high-throughput sequencing contributes a robust tool for the identification of known and unknown virus species in this environment. In the present study, faeces samples from cerrado birds (Psittacara leucophthalmus, Amazona aestiva, and Sicalis flaveola) and mammals (Didelphis albiventris, Sapajus libidinosus, and Galictis cuja) were collected at the Veterinary Hospital, University of Brasília. Viral nucleic acid was extracted, submitted to random amplification, and sequenced by Illumina HiSeq platform. The reads were de novo assembled, and the identities of the contigs were evaluated by Blastn and tblastx searches. Most viral contigs analyzed were closely related to bacteriophages. Novel archaeal viruses of the Smacoviridae family were detected. Moreover, sequences of members of Adenoviridae, Anelloviridae, Circoviridae, Caliciviridae, and Parvoviridae families were identified. Complete and nearly complete genomes of known anelloviruses, circoviruses, and parvoviruses were obtained, as well as putative novel species. We demonstrate that the metagenomics approach applied in this work was effective for identification of known and putative new viruses in faeces samples from Brazilian Cerrado fauna.

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Section: Discussionmentioning

confidence: 50%

Faecal Virome Analysis of Wild Animals from Brazil

Duarte

Silva

Brito

et al. 2019

Viruses

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“…The molecular epidemiology of CAV had been previously described, and several genotypes of CAV had been determined by phylogenetic analysis ( 15 , 16 , 19 , 29 , 30 ). In this study, phylogenetic analysis using full-length CAV genomic sequences was performed according to previous studies.…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Chicken Anemia Virus in Broilers in Shandong Province, China, 2020–2021

Liu

Li²,

Yin

et al. 2022

Front. Vet. Sci.

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Chicken infectious anemia (CIA), caused by chicken anemia virus (CAV), is an immunosuppressive disease characterized by growth retardation, aplastic anemia, lymphoid depletion, and immunodepression in young chickens. In this study, 33 CAV strains were isolated from broilers in Shandong Province during 2020–2021. Phylogenetic analysis of full-length genome sequences showed that most CAV strains isolated in this study were scattered across different branches, but mainly clustered in two genotypes, indicating a certain regional characteristic. Analysis of VP1 protein identified several amino acid substitutions which were relevant with the virulence and virus spread efficiency. Interestingly, four putative DNA recombination events were detected in the genomes of novel isolated CAV strains. In summary, this study demonstrated a genomic diversity of CAV in broilers isolated in Shandong Province during 2020–2021, and provided information for the further study of CAV molecular epidemiology and viral evolution.

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“…These amino acid mutations were universal and mainly concentrated in the Cterminal epitope dominant region, which contained abundant antigenic epitopes. In a word, the above regular variations lead to the formation of different evolutionary subbranches of CIAV strains during the transmission route, and also make isolated genetic evolution most similar to Chinese wild strains in recent years [5], and it has certain guiding signi cance to trace the origin of CIAV isolates clustering based on capsid protein in future research.…”

Section: Discussionmentioning

confidence: 99%

“…Since its rst isolation from contaminated vaccines in Japan [2], it has been transmitted worldwide. Epidemiological investigation revealed that CIAV had also been sporadically distributed in different breeder ocks in China in recent years [3][4][5][6]. CIAV was a non-enveloped virus with a single-stranded circular DNA genome [7], which encodes three partially overlapping ORFs, namely VP1, VP2 and VP3.…”

Section: Introductionmentioning

confidence: 99%

Source Tracking of Chicken Infectious Anemia Virus in a SPF Chicken Population, the Latest Report in China

Wang

Zhang

et al. 2021

Preprint

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BackgroundLive attenuated vaccines have been extensively used to prevent infectious disease in poultry flocks. However, exogenous virus contamination in attenuated vaccines had been reported several times in the past, which brought enormous threat to poultry production and diseases prevention and control. Recently, an attenuated vaccine against Newcastle Disease produced in China was detected contamination with chicken infectious anemia virus (CIAV) in a routine inspection for exogenous virus. To understand the multiple routes of transmission of CIAV, and to better formulate correct prevention and control, it is necessary to find out the possible source of this contamination.Methodslood samples of SPF chickens that generated vaccines were collected to investigate CIAV antibody titers by ELISA test. Then, 14- to 18-day-old SPF chicken embryos (n=40) were randomly selected, DNA was extracted and detected by quantitative real-time PCR and nucleic acid dot hybridization assays. To further investigate the molecular features of the CIAV isolate, the complete genome of CIAV was amplified and analyzed.ResultsThe results showed both SPF chickens and embryos for vaccine preparation were CIAV-positive. In addition, the full-length genome sequences of CIAV from vaccines and SPF chicken embryos were consisted of 2,298 nucleotides (nt) with 100% homology, named as SDSPF2020 (Genbank accession number: MW660821). It demonstrated 95.7%-99.6% homology with the complete nucleotide sequences of reference strains, and shared the closest evolution relationship with the Chinese strain HLJ15125. ConclusionThis study illustrated that vertical transmission of CIAV from SPF chickens and embryos was an important way for exogenous virus contamination in vaccine production. As such, vaccine quality monitoring and health control are significant in the poultry industry from an environmental safety point of view.

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Molecular epidemiology of chicken anaemia virus in sick chickens in China from 2014 to 2015

Cited by 23 publications

References 56 publications

Faecal Virome Analysis of Wild Animals from Brazil

Faecal Virome Analysis of Wild Animals from Brazil

Genomic Characterization of Chicken Anemia Virus in Broilers in Shandong Province, China, 2020–2021

Source Tracking of Chicken Infectious Anemia Virus in a SPF Chicken Population, the Latest Report in China

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