Molecular epidemiology of cytomegalovirus infection in kindergarten children

Shen, Chen‐Yang; Chang, Bao‐Li; Chang, Shu‐Fen; Yang, Show‐Lin; Tseng, Su-Ling; Chen, Chi-Yu; Wu, Cheng‐Wen

doi:10.1002/(sici)1096-9071(199601)48:1<33::aid-jmv5>3.0.co;2-m

Cited by 10 publications

(7 citation statements)

References 18 publications

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“…However, recent evidence has demonstrated that mucosal shedding of HCMV occurs commonly in many healthy individuals (do Canto et al, 2000;Lucht et al, 1998;Shen et al, 1996) and very frequently in immunocompromised patients (Diamond et al, 2000;Fidouh-Houhou et al, 2001;Lucht et al, 1998;Mostad et al, 2000). In this study, RhCMV shedding was detected consistently in many animals, indicating low-level, persistent infection.…”

Section: The Pattern Of Rhcmv Detection Is Very Similar To What Is Knmentioning

confidence: 43%

Differential detection of B virus and rhesus cytomegalovirus in rhesus macaques FN1

Huff

Eberle

Capitanio

et al. 2003

Journal of General Virology

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Section: The Pattern Of Rhcmv Detection Is Very Similar To What Is Knmentioning

confidence: 43%

Differential detection of B virus and rhesus cytomegalovirus in rhesus macaques FN1

Huff

Eberle

Capitanio

et al. 2003

Journal of General Virology

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“…HCV which explains the higher prevalence of CMV DNA in controls than spontaneously cleared HCV patients. In earlier studies on dual viral infections (HBV/CMV and HCV/CMV) [6,23] it was demonstrated that CMV was detected in HBV or HCV patients mostly as a dual infection and that it can aggravate the course of the disease. Recently, Bader El din et al, [14] reported higher rate of CMV co-infection in chronic HCV Egyptian patients than those reported in other patient populations.…”

Section: Discussionmentioning

confidence: 99%

Assessment of human cytomegalovirus co-infection in Egyptian chronic HCV patients

Tabll

Shoman

Ghanem

et al. 2011

Virol J

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Human cytomegalovirus (HCMV) is the most common cause of severe morbidity and mortality in immune- compromised individuals. This study was conducted to determine the incidence of HCMV infection in HCV patients who either spontaneously cleared the virus or progressed to chronic HCV infection. The study included a total of eighty four cases (48 females and 36 males) that were referred to blood banks for blood donation with an age range of 18-64 years (mean age 37.62 ± 10.03 years). Hepatitis C virus RNA and HCMV DNA were detected in sera by RT-nested PCR and nested PCR respectively in all subjects. Immunoglobulin G levels for HCV and HCMV were determined. Besides, IgM antibodies for HCMV infection were also determined in subjects' sera. Fifty three out of 84 cases (63%) were positive for HCV-RNA while 31 (37%) cases had negative HCV RNA. Forty six (87%) and 13 (25%) cases out of 53 HCV RNA positive patients were positive for HCMV IgG and IgM antibodies respectively. While 20 of 53 cases (38%) had detectable HCMV DNA. To examine the role of HCMV infection in HCV spontaneous resolution, two groups of HCV patients, group 1) chronic HCV infection (positive HCV RNA and positive IgG antibodies) vs group 2) spontaneous resolution (negative HCV RNA and positive IgG antibodies) were compared. The percentages of positive CMV IgG and IgM results is higher in chronic HCV patient than those in spontaneously cleared HCV patients and the difference is highly statistically significant (P value < 0.001). Also, there is a general trend towards elevated levels of CMV IgG antibodies in HCV chronic patients than those in spontaneously cleared HCV patients (P value < 0.02). HCMV DNA detection in group 1 was more than twice the value observed in group 2 (38% vs 14.3%, P value < 0.001). Moreover, levels of liver enzymes were significantly higher in HCV RNA positive cases co-infected with HCMV DNA than HCMV negative cases (P value < 0.001). The results indicate the role of HCMV in the liver pathogenesis. We conclude that chronic HCV patients co-infected with HCMV infection can be regarded as high risk groups for liver disease progression where they should be monitored for the long term outcome of the disease.

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“…Previous studies have demonstrated that CMV infection increases in frequency in children attending day-care centers 14 , especially in those for mentally retarded children 20 . The high prevalence of CMV shedding in the urine or saliva by these children, ranging from 39 to 46% in the three virological evaluations performed during the present study, suggests that these secretions are important sources of the virus.…”

Section: Discussionmentioning

confidence: 99%

Cytomegalovirus infection in children with Down syndrome in a day-care center in Brazil

Canto

Granato

Garcez

et al. 2000

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThis study evaluates the transmission of CMV infection in 120 children aged 1 to 15 years with Down syndrome who attended a day-care center for handicapped children in São Paulo, Brazil. A blood sample was obtained from each children at the beginning of the study for detection of IgG and IgM cytomegalovirus (CMV) antibodies by an immunofluorescence assay. Samples of saliva and urine were obtained every 3 months from the children with CMV antibodies to detect shedding of the virus by culture in human foreskin fibroblasts, by detection of pp65 CMV-antigen and by a nested PCR assay.The prevalence of anti CMV-IgG antibodies was 76.6% (92/120), and IgM anti-CMV antibodies were detected in 13% (12/92) of the seropositive children.During the first viral evaluation, CMV was detected in the urine and/or saliva in 39/90 (43.3%) of the seropositive children. In the second and third evaluations, CMV was detected in 41/89 (46%) and in 35/89 (39.3%) children, respectively. Detection of CMV was shown both in urine and saliva in 28/39 (71.8%), 19/41(46.3%) and 20/35 (57.1%) of the children excreting the virus, respectively. Additionally, in 33/49 (67.4%) of the excreters CMV could be demonstrated in urine or saliva in at least two out of the three virological evaluations carried out sequentially in a six month period.Of the 28 initially seronegative children, 26 were re-examined for anti-CMV IgG antibodies about 18 months after the negative sample; seroconversion was found in 10/26 (38.5%).Taking all 536 samples of urine or saliva examined by virus culture and pp65 antigen detection during the study into account, 159 (29.6%) were positive by virus culture and 59 (11%) gave a positive result with the pp65 assay.These data demonstrate the high prevalence of CMV shedding and the high risk of CMV infection in children with Down syndrome attending a day-care center for mentally handicapped patients. The virus culture was more sensitive than the pp65 CMV antigen assay for CMV detection in both urine and saliva samples.

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Molecular epidemiology of cytomegalovirus infection in kindergarten children

Cited by 10 publications

References 18 publications

Differential detection of B virus and rhesus cytomegalovirus in rhesus macaques FN1

Differential detection of B virus and rhesus cytomegalovirus in rhesus macaques FN1

Assessment of human cytomegalovirus co-infection in Egyptian chronic HCV patients

Cytomegalovirus infection in children with Down syndrome in a day-care center in Brazil

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