Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli

Johnson, James R.; Russo, Thomas A.

doi:10.1016/j.ijmm.2005.07.005

Cited by 226 publications

(241 citation statements)

References 220 publications

(229 reference statements)

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“…6 Group B2 accounted for six clones, group B1 for seven clones and groups D and A for one clone each. The six clones (ST 223,815,27,29,31,287) considered uropathogens (labeled UTI-associated in Table 1) were predominatly Group B2.…”

Section: Phylogenetic Groups and Virulence Genesmentioning

confidence: 99%

“…4,5 Several studies have delineated characteristics of E. coli which cause extraintestinal disease. 6 Clones belonging to phylogenetic group B2 and to a lesser extent group D express certain virulence genes (pap, sfa/foc, hly and kps) and are commonly isolated from patients with pyelonephritis, bacteremia and/or meningitis. In contrast, intestinal clones within phylogenetic groups A or B1 express few virulence factors and are commonly isolated from the fecal flora of healthy hosts.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, intestinal clones within phylogenetic groups A or B1 express few virulence factors and are commonly isolated from the fecal flora of healthy hosts. 6 Multilocus sequence typing (MLST) has become the gold standard for investigating the genetic relationship between pathogenic strains of E. coli and reference strains. 7 MLST uses nucleotide sequences of internal fragments of selected genes as the unit of comparison.…”

Section: Introductionmentioning

confidence: 99%

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Escherichia coli colonizing the neurogenic bladder are similar to widespread clones causing disease in patients with normal bladder function

et al. 2008

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Study design: Clonal typing of neurogenic clones. Objective: To determine whether neurogenic clones carried over weeks in the urine of asymptomatic children with neurogenic bladder were similar to known uropathogenic clones associated with disease. Setting: Michigan State University; VA Medical Center, Minneapolis, MN, USA. Methods: Escherichia coli isolates from the urine of 15 children previously followed were typed by multilocus sequence typing and compared to 2 human pyelonephritis genome strains, 29 pediatric or adult symptomatic urinary tract infection strains, 15 pediatric asymptomatic bacteriuria strains, 6 animal urinary tract infection strains and a neonatal meningitis-septicemia prototype K1 strain. Phylotypes and virulence genotypes were determined using PCR. Results: Twenty-nine E. coli isolates were classified into 15 clones. Six of 15 clones were the same sequence type or a close relative to a clone that caused disease in a human or animal. These clones were considered uropathogens. The remaining nine clones were not closely related to a clone that caused disease and were considered commensal clones. Uropathogens were predominantly group B2, exhibited more virulence genes and were carried for more weeks in the neurogenic bladder compared to commensal clones. Nine of 15 children carried one or more uropathogenic clones; 4 children carried one or more commensal clones and 2 children carried both uropathogenic and commensal clones. Conclusion: Children with neurogenic bladder most commonly carried commensal clones. Uropathogenic clones were associated with prolonged carriage, however, carriage was not associated with symptomatic disease or deterioration of the upper urinary tract.

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Section: Phylogenetic Groups and Virulence Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Escherichia coli colonizing the neurogenic bladder are similar to widespread clones causing disease in patients with normal bladder function

et al. 2008

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“…To create an infection, fecal bacteria colonize at the entrance of the urinary tracts where they can enter into the internal organs such as bladder and kidney to make infections. Regarding the origin of intestinal bacteria and their moving out of the digestive system due to change in the conditions and horizontal transfer, they undergo some genetic differences [1,10,12]. Enteroaggregative E. coli (EAEC) is a pathogenic strain in the digestive tract that causes a severe and stable diarrhea [3].…”

Section: Introductionmentioning

confidence: 99%

Distribution of Pathogenic Genes aatA, aap, aggR, among Uropathogenic Escherichia coli (UPEC) and Their Linkage with StbA Gene

et al. 2011

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Urinary tract infection (UTI) with E. coli (UPEC) is one of the most common bacterial infections among human beings. In addition to the host predisposing factors, genes are also proposed to have an important role in the occurrence of UTIs. This study investigated the distribution of three pathogenic genes including aggR, aap and aatA among UPEC infected samples and their linkage with stbA, the essential gene for maintaining of pAA plasmid. A total of 244 samples were collected from patients with UTIs through clinical laboratories located in western side of Tehran (Iran) during years [2008][2009]. E. coli isolation was performed according to standard laboratory methods. DNAs were extracted from samples using Boiling method, and the presence of aap, aggR, aatA and stbA genes were investigated by PCR. No pathogenic genes (aap, aggR, aatA) were found in 104 out of 244 UPEC samples, while 14 of them were carrying stbA gene. Out of 140 UPEC samples with pathogenic genes, 94 (46.6%) were carrying aap gene, 52 (23%) aggR gene, and 80 (35.4%) aatA gene. A total of 18 samples were also carrying all pathogenic genes together. Moreover, 44 out of 144 samples were carrying stbA gene. The results obtained by this study showed that the aggR, aap and aatA pathogenic genes have different existence patterns in different E. coli strains that infect different organs. Our study also showed that these three plasmid genes in EAEC strains are able to transpose in the genome and change their level of linkage with pAA plasmid essential gene stbA. Meanwhile, this study confirmed that aggR, aap and aatA genes are not specific to only EAEC strains.

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“…Extraintestinal pathogenic Escherichia coli (ExPEC) cause most community and hospital-associated extraintestinal E. coli infections (Johnson and Russo, 2005). Specific ExPEC lineages, or sequence types (STs), are commonly multidrug-resistant (MDR), and have been associated with international travel and medical care.…”

Section: Introductionmentioning

confidence: 99%

Clonal distribution and associated characteristics of Escherichia coli clinical and surveillance isolates from a military medical center

Manges

Mende

Murray

et al. 2017

Diagnostic Microbiology and Infectious Disease

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Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli

Cited by 226 publications

References 220 publications

Escherichia coli colonizing the neurogenic bladder are similar to widespread clones causing disease in patients with normal bladder function

Escherichia coli colonizing the neurogenic bladder are similar to widespread clones causing disease in patients with normal bladder function

Distribution of Pathogenic Genes aatA, aap, aggR, among Uropathogenic Escherichia coli (UPEC) and Their Linkage with StbA Gene

Clonal distribution and associated characteristics of Escherichia coli clinical and surveillance isolates from a military medical center

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