Molecular epidemiology of Giardia and Cryptosporidium infections in dairy calves originating from three sources in Western Australia

Becher, K.A; Robertson, Ian D.; Fraser, D M; Palmer, D.G.; Thompson, R.C.A.

doi:10.1016/j.vetpar.2004.05.020

Cited by 108 publications

(90 citation statements)

References 34 publications

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“…Although Barwick et al (2003) reported that the environmental contamination of farms with oocysts was insufficient to account for the high levels of infection seen in cattle, contaminated soil is considered to be a sufficient infection source for newly introduced animals. Other studies support the importance of calfto-calf contact in transmission (Xiao et al, 1993;O'Handley et al, 1999;Wade et al, 2000;Becher et al, 2004). The present work is the first report on Cryptosporidium occurrence in calves in Tunisia associating molecular characterization of few parasite isolates.…”

Section: Discussionsupporting

confidence: 77%

Cryptosporidium parvum(Eucoccidiorida: Cryptosporiidae) in calves: results of a longitudinal study in a dairy farm in Sfax, Tunisia

Soltane¹,

Guyot

Dei‐Cas

et al. 2007

Parasite

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Summary :A longitudinal study was undertaken to determine the prevalence of Cryptosporidium in a dairy farm in Sfax, Tunisia. 480 faecal samples were obtained from 30 calves under one month of age. All faecal samples were analysed for Cryptosporidium oocysts by microscopic examination of smears stained by modified Ziehl Neelsen technique. The parasite was detected in 26 calves (86.7 %). Infection was significantly associated with diarrhoea. A molecular characterization, performed in seven calves, confirmed that isolates were C. parvum. This work is the first report on Cryptosporidium in calves in Tunisia. Résumé

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Section: Discussionsupporting

confidence: 77%

Cryptosporidium parvum(Eucoccidiorida: Cryptosporiidae) in calves: results of a longitudinal study in a dairy farm in Sfax, Tunisia

Soltane¹,

Guyot

Dei‐Cas

et al. 2007

Parasite

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“…For reasons of comparison, it might therefore, be interesting to study Giardia assemblages from asymptomatic patients. The majority of G. duodenalis in livestock belongs to Assemblage E and a minority to Assemblage A (Ey et al, 1997, Hunt et al, 2000, Becher et al, 2004and Read et al, 2004Appelbee et al, 2005;Giangaspero et al, 2005). However, in most studies the molecular identification was based on A-and B-specific PCR and not on analysing the gdh-derived DNA sequence product.…”

Section: Discussionmentioning

confidence: 99%

Genotyping of Giardia in Dutch patients and animals: A phylogenetic analysis of human and animal isolates

Giessen

Vries

Roos

et al. 2006

International Journal for Parasitology

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Giardia duodenalis (syn. Giardia lamblia, Giardia intestinalis) is a protozoan organism that can infect the intestinal tract of many animal species including mammals. Genetic heterogeneity of G. duodenalis is well described but the zoonotic potential is still not clear. In this study, we analysed 100 Giardia DNA samples directly isolated from human stool specimens, to get more insight in the different G. duodenalis assemblages present in the Dutch human population. Results showed that these human isolates could be divided into two main Assemblages A and B within the G. duodenalis group on the basis of PCR assays specific for the Assemblages A and B and the DNA sequences of 18S ribosomal RNA and the glutamate dehydrogenase (gdh) genes. Genotyping results showed that G. duodenalis isolates originating from Dutch human patients belonged in 35% of the cases to Assemblage A (34/98) and in 65% of the cases to Assemblage B (64/98) whereas two human cases remained negative in all assays tested. In addition, we compared these human samples with animal samples from the Netherlands and human and animal samples from other countries. A phylogenetic analysis was carried out on the DNA sequences obtained from these Giardia and those available in GenBank. Using gdh DNA sequence analysis, human and animal Assemblage A and B Giardia isolates could be identified. However, phylogenetic analysis revealed different sub-clustering for human and animal isolates where host-species-specific assemblages (C, D, E, F and G) could be identified. The geographic origin of the human and animal samples was not a discriminating factor.

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“…We did not consider different seasons in our study, but our findings reveals no statistically difference for Cryptosporidium infection rate in various weather conditions of Lorestan province. Becher et al (2004) showed that season had no effect on prevalence of the infection. There are many different deductions represented by other works on effects of season on prevalence of cryptosporidiosis.…”

Section: Discussionmentioning

confidence: 97%

Prevalence of Cryptosporidium spp. in ruminants of Lorestan province, Iran

et al. 2015

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Cryptosporidiosis is one of the important zoonotic diseases caused by an intracellular protozoan parasite called Cryptosporidium. This study aimed to investigate the prevalence of Cryptosporidium spp. infection on 1,115 ruminants, cattle, sheep and goats, in Lorestan province, Iran. Using formol-ether concentration technique and modified Ziehl-Neelsen staining method afterwards, the overall prevalence of Cryptosporidium spp. infection in ruminants of Lorestan province was 7.17 %. Prevalence of infection was 9.07 % (39 of 430), 5.80 % (20 of 345) and 6.18 % (21 of 340) for cattle, sheep and goats respectively. There was no significant difference between contamination of all examined animals and different geographical and climatic situations (P [ 0.05) and diarrhea was not directly associated with Cryptosporidium infection (P [ 0.05). In conclusion, the prevalence of cryptosporidiosis in Lorestan province was relatively low, but it should be noticed that this opportunistic parasite is zoonosis and also can make epidemics in ruminants as well as human population in suitable conditions.

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Molecular epidemiology of Giardia and Cryptosporidium infections in dairy calves originating from three sources in Western Australia

Cited by 108 publications

References 34 publications

Cryptosporidium parvum(Eucoccidiorida: Cryptosporiidae) in calves: results of a longitudinal study in a dairy farm in Sfax, Tunisia

Cryptosporidium parvum(Eucoccidiorida: Cryptosporiidae) in calves: results of a longitudinal study in a dairy farm in Sfax, Tunisia

Genotyping of Giardia in Dutch patients and animals: A phylogenetic analysis of human and animal isolates

Prevalence of Cryptosporidium spp. in ruminants of Lorestan province, Iran

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