Molecular epidemiology of hepatitis A virus infections in Catalonia, Spain, 2005–2009: Circulation of newly emerging strains

Pérez-Sautu, Unai; Costafreda, María Isabel; Lite, Josep; Sala, Rosa; Barrabeig, Irene; Bosch, Albert; Pintó, Rosa M.

doi:10.1016/j.jcv.2011.06.011

Cited by 53 publications

(51 citation statements)

References 19 publications

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“…The recently proposed subgenotype IC [14] isolated from Peru and Spain was also included in the phylogenetic analysis. Results indicated that the isolates from China in this study did not belong to the new IC subgenotype based on the current genotyping methods.…”

Section: Resultsmentioning

confidence: 99%

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Genetic Diversity of Hepatitis A Virus in China: VP3-VP1-2A Genes and Evidence of Quasispecies Distribution in the Isolates

et al. 2013

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Hepatitis A virus (HAV) is the most common cause of infectious hepatitis throughout the world, spread largely by the fecal-oral route. To characterize the genetic diversity of the virus circulating in China where HAV in endemic, we selected the outbreak cases with identical sequences in VP1-2A junction region and compiled a panel of 42 isolates. The VP3-VP1-2A regions of the HAV capsid-coding genes were further sequenced and analyzed. The quasispecies distribution was evaluated by cloning the VP3 and VP1-2A genes in three clinical samples. Phylogenetic analysis demonstrated that the same genotyping results could be obtained whether using the complete VP3, VP1, or partial VP1-2A genes for analysis in this study, although some differences did exist. Most isolates clustered in sub-genotype IA, and fewer in sub-genotype IB. No amino acid mutations were found at the published neutralizing epitope sites, however, several unique amino acid substitutions in the VP3 or VP1 region were identified, with two amino acid variants closely located to the immunodominant site. Quasispecies analysis showed the mutation frequencies were in the range of 7.22x10-4 -2.33x10-3 substitutions per nucleotide for VP3, VP1, or VP1-2A. When compared with the consensus sequences, mutated nucleotide sites represented the minority of all the analyzed sequences sites. HAV replicated as a complex distribution of closely genetically related variants referred to as quasispecies, and were under negative selection. The results indicate that diverse HAV strains and quasispecies inside the viral populations are presented in China, with unique amino acid substitutions detected close to the immunodominant site, and that the possibility of antigenic escaping mutants cannot be ruled out and needs to be further analyzed.

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Section: Resultsmentioning

confidence: 99%

“…Genotypes I, II, and III are found in humans, and each of them is further divided into subgenotypes A and B. Recently subgenotype IC isolated from Peru and Spain has been proposed [14]. Genotype I is most prevalent worldwide, and sub-genotype IA is more common than IB.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Hepatitis A Virus in China: VP3-VP1-2A Genes and Evidence of Quasispecies Distribution in the Isolates

et al. 2013

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“…In recent years, numerous outbreaks of acute hepatitis A have occurred in men who have sex with men (MSM) in Europe, United States and Japan, with specific HAV IIIA strains circulating among MSM communities identified by phylogenetic trees …”

Section: Phylogenesis and Phylodynamic Of Hepatitis A Virusmentioning

confidence: 99%

The phylogenetic approach for viral infectious disease evolution and epidemiology: An updating review

Ciccozzi

Lai

Zehender

et al. 2019

Journal of Medical Virology

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In the last decade, the phylogenetic approach is recurrent in molecular evolutionary analysis. On 12 May, 2019, about 2 296 213 papers are found, but typing “phylogeny” or “epidemiology AND phylogeny” only 199 804 and 20 133 are retrieved, respectively. Molecular epidemiology in infectious diseases is widely used to define the source of infection as so as the ancestral relationships of individuals sampled from a population. Coalescent theory and phylogeographic analysis have had scientific application in several, recent pandemic events, and nosocomial outbreaks. Hepatitis viruses and immunodeficiency virus (human immunodeficiency virus) have been largely studied. Phylogenetic analysis has been recently applied on Polyomaviruses so as in the more recent outbreaks due to different arboviruses type as Zika and chikungunya viruses discovering the source of infection and the geographic spread. Data on sequences isolated by the microorganism are essential to apply the phylogenetic tools and research in the field of infectious disease phylodinamics is growing up. There is the need to apply molecular phylogenetic and evolutionary methods in areas out of infectious diseases, as translational genomics and personalized medicine. Lastly, the application of these tools in vaccine strategy so as in antibiotic and antiviral researchers are encouraged.

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“…En varios estudios se determinó que las cepas o variantes del patógeno encontradas en inmigrantes tienen su origen más probable en los países de origen, como en el estudio del virus de la hepatitis A ligado a un brote alimentario 8 , de la hepatitis B 25,26 , de la hepatitis E 7 , del HTLV 10 y en el estudio de varios virus de transmisión por vía sanguínea 31 . Semejantes conclusiones se indicaban en los más numerosos estudios de VIH 9,18,32-37 y en algunos que estudiaban M. tuberculosis 21,38,39 , si bien también se pusieron de manifiesto casos de transmisión desde la población autóctona a la inmigrante 3,22,23,39 .…”

Section: Figura 3 Distribución De Los Trabajos Publicados Sobre Epideunclassified

“…No obstante, dependiendo del patógeno y de los objetivos iniciales del estudio molecular, todavía se encuentran trabajos basados en estas aproximaciones. La facilidad para la obtención de secuencias génicas, incluso genómicas, ha permitido que la epidemiología molecular basada en el conocimiento de estas secuencias sea la metodología más extendida, bien de forma directa, especialmente para el estudio de virus [7][8][9][10] , o bien tras su codificación en variantes discretas, como en los sistemas de tipado multilocus (MLST) adoptados en muchas bacterias.…”

Section: Introductionunclassified

Estudios de epidemiología molecular en población inmigrante en España

Gónzález-Candelas¹,

Bracho²,

Comas³

et al. 2014

Rev. Esp. Salud Publica

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RESUMENFundamentos: La epidemiología molecular es una nueva disciplina que permite la integración de la información sobre la variabilidad genéti-ca de patógenos infecciosos con su difusión en la población y subgrupos de la misma incluyendo, por ejemplo, las mutaciones de resistencia a antibióticos y antivirales. El objetivo es conocer qué posibles diferencias existe en las características genéticas de los agentes infecciosos que afectan a las poblaciones inmigrante y autóctoctona en España.. Métodos:Se revisaron artículos originales publicados entre 1998-2013, con las palabras clave "epidemiología molecular", "tipado molecular", "secuenciación", "inmigrante", "España".Resultados: De un total de 267 artículos identificados inicialmente, 50 pasaron los diferentes filtros establecidos. De ellos, 36 analizan las infecciones por Mycobacterium tuberculosis y VIH, seguidos de los que analizan infecciones por Staphylococcus aureus (3) y el Virus de la Hepatitis B (3).Conclusiones: Los objetivos principales de estos trabajos fueron el tipado del patógeno y la determinación de la frecuencia de mutaciones de resistencia. Los estudios más frecuentes correspondieron a cohortes retrospectivas, seguidos por los estudios ecológicos y los ensayos clínicos. En general los estudios son descriptivos y su ámbito por el tipo y tamaño de muestra es bastante restringido. En varios se determina que las cepas o variantes del patógeno encontradas en inmigrantes tienen su origen más probable en sus países de origen, si bien otros también ponen de manifiesto la transmisión desde la población autóctona a la inmigrante. ABSTRACT Molecular Epidemiology Studies on the Immigrant Population in SpainBackground: Molecular epidemiology is a new scientific discipline which allows to integrate information on the genetic variation of infectious pathogens with their diffusion in a population and its subgroups including, for instance, resistance mutations to antibiotics and antiretrovirals. We present the results of an analysis of scientific publications that analyze the health status of the immigrant population in Spain from a molecular epidemiology perspective.

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Molecular epidemiology of hepatitis A virus infections in Catalonia, Spain, 2005–2009: Circulation of newly emerging strains

Cited by 53 publications

References 19 publications

Genetic Diversity of Hepatitis A Virus in China: VP3-VP1-2A Genes and Evidence of Quasispecies Distribution in the Isolates

Genetic Diversity of Hepatitis A Virus in China: VP3-VP1-2A Genes and Evidence of Quasispecies Distribution in the Isolates

The phylogenetic approach for viral infectious disease evolution and epidemiology: An updating review

Estudios de epidemiología molecular en población inmigrante en España

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