Molecular Epidemiology of Human Astrovirus Diarrhea among Children from a Periurban Community of Mexico City

Walter, Jolán E.; Mitchell, D.; Guerrero, M. Lourdes; Berke, Tamás; Matson, David O.; Monroe, Stephan S.; Pickering, Larry K.; Ruíz-Palacios, Guillermo M.

doi:10.1086/318825

Cited by 81 publications

(92 citation statements)

References 31 publications

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“…The infection rate ranged from 2 to 6 % for hospitalized children with acute diarrhoea and 5-17 % for acute diarrhoea cases from the community [3]. In both developed and developing countries, HAstV infection was associated with diarrhoea episodes and ranged from 3 to 12 and 2 to 26 % respectively [18,23,29,34,40,41].…”

Section: Astrovirus Detection In Various Settingsmentioning

confidence: 99%

“…Recombination events have been observed in astroviruses which infect both human and animals. Novel recombinant human astrovirus strains were first described in children with HAstV_G3 (ORF1b) and HAstV_G5 (ORF2) combination in the ORF1b/ORF2 junction [40]. Human astrovirus wild-type strain obtained from a Kenyan child showed genetic recombination event in the sequence analyses of ORF1a (HAstV_G6/7), ORF1b (HAstV_G3) and ORF2 region (HAstV_G2) [42].…”

Section: Genomic Diversity Among Astrovirusesmentioning

confidence: 99%

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Novel human astroviruses: challenges for developing countries

Krishnan

2014

VirusDis.

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Astroviruses have been gaining widespread importance over the past few decades owing to their detection through advanced molecular techniques. The association of astrovirus-associated enteric infections have been reported from various settings among different age groups. The tremendous efforts of scientists from different countries to detect and characterize these star-like viruses in the course of surveillance has shown the emergence of novel astroviruses from varied host species, necessitating changes in the classification to update their taxonomy. The public health importance of these viruses implies new control measures are essential to reduce disease burden in developing countries.

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Section: Astrovirus Detection In Various Settingsmentioning

confidence: 99%

Section: Genomic Diversity Among Astrovirusesmentioning

confidence: 99%

Novel human astroviruses: challenges for developing countries

Krishnan

2014

VirusDis.

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“…These small, spherical (28-30 nm in diameter), non-enveloped viruses have a plus sense, single-stranded polyadenylated RNA (ssRNA) 13 genome of about 7 kb in length . To date, eight HAstVs genotypes (HAstV-1 to HAstV-8) have been described, and HAstV-1 has been reported as the most prevalent type in 6,46 both developing and developed countries .…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

et al. 2010

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Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil Caracterização molecular de norovírus, sapovírus e astrovírus em crianças com gastroenterite aguda em Belém, Pará, Brasil Caracterización molecular de norovirus, sapovirus y astrovirus en niños con gastroenteritis aguda en Belém (Pará, Brasil) http://revista.iec.pa.gov.br Glicélia Cruz Aragão ABSTRACTThe importance of norovirus (NoVs), sapovirus (SaVs) and human astrovirus (HAstVs) as causes of gastroenteritis outbreaks is already well-defined, but a few studies have described sporadic cases of acute gastroenteritis caused by these viral entities. The aim of this study was to determine the role of these viruses in the etiology of acute gastroenteritis in children enrolled to participate in hospital -and emergency department -based intensive surveillance carried out in Belém, Brazil, from March to September 2003. A total of 305 stool specimens from patients with severe gastroenteritis were collected and screened by reverse transcription followed by polymerase chain reaction (RT-PCR), using the specific primers Mon 269 and Mon 270 for HAstVs, p289 and p290 for human calicivirus (HuCVs), and Mon 431/433 and Mon 432/434 for NoVs. Sequencing of RT-PCR HAstV, HuCV and NoV amplicons was carried out using the same primers. Of the 305 samples tested, 96 (31.5%) were positive, with 51 diagnosed as HuCVs, 40 as HAstVs and five as mixed infections. Of the 56 (18.4%) HuCVs sequenced, 30 were NoVs (9.8%) of genogroups and 15 (4.9%) were SaVs of types GI-1, GI-2 and GII-1. HAstVs, including genotypes 1, 8 and 2, were detected in 45 (14.7%) samples. This study has highlighted the importance of these viruses as causes of acute gastroenteritis and established the circulation of different genotypes during the study period. These results reinforce the need for establishing an intensive surveillance for gastroenteritis caused by these viruses to assess the burden of disease and to monitor the circulation of genotypes.

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“…The HAstV genome is a polyadenylated plus-strand RNA molecule of ϳ7 kb organized in three open reading frames (ORFs): ORF1a and ORF1b, at the 5Ј end of the genome, code for the nonstructural viral proteins, while ORF2, at the 3Ј end, encodes the capsid proteins (8). Based on the nucleotide and encoded amino acid sequences of either the amino-terminal or carboxy-terminal region of ORF2, these viruses have been grouped into eight genotypes (11,16), which have been shown to completely correlate with the eight established HAstV serotypes (9,10,12,24,28; J. B. Kurtz and T. W. Lee, Letter, Lancet ii:1405, 1984).…”

mentioning

confidence: 99%

Prevalence and Genetic Diversity of Human Astroviruses in Mexican Children with Symptomatic and Asymptomatic Infections

et al. 2004

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The prevalence and type diversity of human astroviruses (HAstV) in children with symptomatic and asymptomatic infections were determined in five localities of Mexico. HAstV were detected in 4.6 (24 of 522) and 2.6% (11 of 428) of children with and without diarrhea, respectively. Genotyping of the detected strains showed that at least seven (types 1 to 4 and 6 to 8) of the eight known HAstV types circulated in Mexico between October 1994 and March 1995. HAstV types 1 and 3 were the most prevalent in children with diarrhea, although they were not found in all localities studied. HAstV type 8 was found in Mexico City, Monterrey, and Mérida; in the last it was as prevalent (40%) as type 1 viruses, indicating that this astrovirus type is more common than previously recognized. A correlation between the HAstV infecting type and the presence or absence of diarrheic symptoms was not observed. Enteric adenoviruses were also studied, and they were found to be present in 2.3 (12 of 522) and 1.4% (6 of 428) of symptomatic and asymptomatic children, respectively.

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Molecular Epidemiology of Human Astrovirus Diarrhea among Children from a Periurban Community of Mexico City

Cited by 81 publications

References 31 publications

Novel human astroviruses: challenges for developing countries

Novel human astroviruses: challenges for developing countries

Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

Prevalence and Genetic Diversity of Human Astroviruses in Mexican Children with Symptomatic and Asymptomatic Infections

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