Molecular Epidemiology of Brucella Genotypes in Patients at a Major Hospital in Central Peru

Abstract

The multiple-locus variable-number repeat analysis of 90 human Brucella melitensis isolates from a large urban area in central Peru revealed variations at 4 (Bruce07, Bruce09, Bruce18, and Bruce42) out of 16 loci investigated, of which 1 (Bruce42) also is used for species identification. Ten genotypes were identified, separated by the number of Bruce42 repeats into two groups that may have distinct phenotypic characteristics. Whereas genotypes with five or six Bruce42 repeats were cultured mainly from adult patients, genotypes with three Bruce42 repeats were isolated from children and young adolescents as well as from adults. In addition, the isolates with three Bruce42 repeats were obtained more often from patients with splenomegaly (P ‫؍‬ 0.02) or hepatomegaly (P ‫؍‬ 0.006). An annual variation in the diversity of genotypes was observed, possibly reflecting changes in sources of fresh dairy products, supply routes to city shops and markets, and the movement of infected dairy goat herds.

Multiple-Locus Variable-Number Tandem-Repeat Analysis Genotyping of Human Brucella Isolates from Turkey

“…In agreement with the previous investigations (1,11,18), the MLVA-16 Orsay genotyping results showed good correlation with the epidemiological data. The present findings also confirmed relapses, laboratory-acquired brucellosis, and intrafamiliary brucellosis resulting from food sharing.…”

“…With regard to MLVA-8 Orsay genotypes, the most common genotypes (42 and 43) found in the current study were also observed in other parts of the world (1,11,18,23). In contrast, those of the typical West Mediterranean family, including MLVA-8 Orsay genotypes 49 and 51, were not detected in this study.…”

“…The MLVA-16 Orsay assay has been shown to be an appropriate method for species identification in the Brucella genus, for discriminating isolates originating from restricted geographic sources at the subspecies level, and for trace-back analyses (1,14). This method has been reported to be highly discriminatory to distinguish strains within a local outbreak, and to some extent phylogenetically relevant (1,11,14,16,18,23) and typing data from several hundred isolates can be queried and accessed via the Internet (http://mlva.u-psud.fr). The genetic diversity of Brucella strains isolated from human and animal infection has not yet been investigated in Turkey.…”

Epidemiological and Phylogenetic Analysis of Spanish HumanBrucella melitensisStrains by Multiple-Locus Variable-Number Tandem-Repeat Typing, Hypervariable Octameric Oligonucleotide Fingerprinting, andrpoBTyping

“…Based on tandem repeats, both hypervariable octameric oligonucleotide fingerprinting (HOOF-prints) (5) and multilocus variable-number tandem-repeat analysis (MLVA-16) (1, 18) have proven their discriminatory power in the study of Brucella species collected on a global scale (18,19,32). Recent studies have shown the latter technique to be useful in studies of different geographic and time sampling characteristics involving B. abortus (13) and B. melitensis (17,21,22,28,29).…”

A Foodborne Outbreak of Brucellosis at a Police Station Cafeteria, Lima, Peru

“…Culture isolates were genotyped independently by laboratories at the Universidad Peruana Cayetano Heredia and NAMRU-6 (Lima, Peru) using multiple locus variable number tandem repeat analysis (MLVA-16 locus). 22,23 Isolates obtained during follow-up were tested using the previously described five polymorphic loci in Peruvian B. melitensis biovar 1 isolates (Bruce 07, 09, 16, 18, and 42). 23 A 100 bp and a 20 bp molecular marker ladder (Invitrogen, Carlsbad, CA) were used to estimate MLVA band sizes; the B. melitensis strain 16 M was used as the control.…”