Molecular Epidemiology of<i>Streptococcus uberis</i>Isolates from Dairy Cows with Mastitis

Phuektes, Patchara; Mansell, P.D.; Dyson, Rodney S.; Hooper, Narelle D.; Dick, Jennifer S.; Browning, Glenn F.

doi:10.1128/jcm.39.4.1460-1466.2001

Cited by 94 publications

(153 citation statements)

References 25 publications

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“…The strain collection contained 57 different sequence types (STs), showing that the population was heterogeneous. This is in agreement with a number of other studies using different typing methods to differentiate sets of S. uberis isolates (5,10,15,17). However, the MLST analysis, unlike most previous typing methods, provided useful information about the genetic relationships between the strains.…”

supporting

confidence: 79%

Application of Streptococcus uberis Multilocus Sequence Typing: Analysis of the Population Structure Detected among Environmental and Bovine Isolates from New Zealand and the United Kingdom

Pullinger

Lopez-Benavides²,

Coffey

et al. 2006

Appl Environ Microbiol

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We recently developed a multilocus sequence typing (MLST) scheme to differentiate S. uberis isolates and facilitate an understanding of the population biology of this pathogen. The scheme was initially used to study a collection of 160 bovine milk isolates from the United Kingdom and showed that the majority of isolates were from one clonal complex (designated the ST-5 complex). Here we describe the MLST analysis of a collection of New Zealand isolates. These were obtained from diverse sources, including bovine milk, other bovine anatomical sites, and environmental sources. The complete allelic profiles of 253 isolates were determined. The collection was highly diverse and included 131 different sequence types (STs). The New Zealand and United Kingdom populations were distinct, since none of the 131 STs were represented within the previously studied collection of 160 United Kingdom S. uberis isolates. However, seven of the STs were members of the ST-5 clonal complex, the major complex within the United Kingdom collection. Two new clonal complexes were identified: ST-143 and ST-86. All three major complexes were isolated from milk, other bovine sites, and the environment. Carriage of the hasA gene, which is necessary for capsule formation, correlated with clonal complex and isolation from clinical cases of mastitis.

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supporting

confidence: 79%

Application of Streptococcus uberis Multilocus Sequence Typing: Analysis of the Population Structure Detected among Environmental and Bovine Isolates from New Zealand and the United Kingdom

Pullinger

Lopez-Benavides²,

Coffey

et al. 2006

Appl Environ Microbiol

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“…Nevertheless, the response time to infection may vary according to the pathogenic variability of the strains and their resistance to antibiotics, as well as to the animals' own resistance. That variability was especially revealed with Streptococcus uberis [22,26,37].…”

Section: Discussionmentioning

confidence: 99%

Effect of mastitis and related-germ on milk yield and composition during naturally-occurring udder infections in dairy cows

Coulon

Gasqui

Barnouin³

et al. 2002

Anim. Res.

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-The effect of mastitis and related-germs on milk chemical composition (protein and lactose contents) and milk somatic cell count (SCC) was investigated in 501 milk quarter samples during two consecutive years in cows from three experimental herds. Each infected quarter was matched by a healthy one in the same udder, as a control. Milk protein and mineral assays were performed in a subsample of 128 milks. Staphylococci were the most frequently isolated germs (Staphylococcus aureus: 27%, coagulase-negative Staphylococci: 26%, Streptococci: 21%). Major milk pathogens (Staphylococcus aureus, Streptococcus uberis or Escherichia coli) associated with clinical signs of mastitis were accompanied by higher SCC (+1.6 log·mL -1 , P < 0.01), lower lactose concentration (-7.6 g·kg -1 , P < 0.01), higher protein concentration (+3.3 g·kg -1 , P < 0.01) and higher soluble protein concentrations (IgG and BSA), hence a sharp decrease in the casein/protein ratio (-10 percentage points, P < 0.01). Changes were more marked when Escherichia coli was present. Corynebacterium bovis did not alter milk chemical composition whereas coagulase-negative Staphylococci slightly reduced lactose concentration (-1.8 g·kg -1 ) and increased SCC (+0.37 log·mL -1 ). Calcium and phosphorus milk contents were hardly modified by the presence of microorganisms. The decrease in milk yield during clinical mastitis varied from 1.6 kg·d -1 in the presence of Staphylococcus aureus to 15 kg·d -1 in the presence of Escherichia coli. mastitis / milk composition / microorganismRésumé -Effet du type de mammite et du germe sur la production et la composition du lait lors d'infections mammaires naturelles chez la vache laitière. L'effet de la nature des germes pathogènes présents dans le lait sur la composition chimique (taux protéique, taux de lactose) et la numération cellulaire du lait a été étudié à partir d'un échantillon de 501 laits de quartiers prélevés au *Correspondence and reprints E-mail: jbc@clermont.inra.fr cours de 2 années consécutives sur les vaches de 3 troupeaux expérimentaux. Pour chaque quartier infecté, un quartier sain de la même mamelle a servi de témoin. Sur 128 de ces prélèvements, des analyses de la composition minérale et protéique des laits ont été réalisées. Les germes les plus fréquemment observés ont été les staphylocoques (27 % de Staphylococcus aureus et 26 % de staphylocoques à coagulase négative) et les streptocoques (21 %). Lorsqu'elle a été associée à des signes de mammites cliniques, la présence d'un germe majeur (Staphylococcus aureus, Streptococcus uberis ou Escherichia coli) s'est accompagnée d'une augmentation de la numération cellulaire (+1,6 log·mL -1 , P < 0,01), d'une diminution de la teneur en lactose (-7,6 g·kg -1 , P < 0,01), d'une augmentation du taux protéique (+3,3 g·kg -1 , P < 0,01) et du taux de protéines solubles (IgG et BSA), de sorte que le rapport caséines/protéines a fortement diminué (-10 points de pourcentage, P < 0,01). Ces modifications ont été les plus importantes en présence d'Escherichia ...

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“…S. uberis has been recovered from soil, bedding materials, feces, as well as, various anatomical regions of the cow [7,8]. Similar to S. marcescens infections, IMI's caused by S. uberis are predominantly subclinical and can persist for long periods of time in a chronic state [21,22,40]. Currently recommended antibiotic therapy in the USA for S. uberis IMI remains sub-optimal as two-day treatment with pirlimycin was recently reported to eliminate only 58% of infections [38].…”

Section: Introductionmentioning

confidence: 99%

Innate immune response to intramammary infection with Serratia marcescens and Streptococcus uberis

et al. 2004

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-Streptococcus uberis and Serratia marcescens are Gram-positive and Gram-negative bacteria, respectively, that induce clinical mastitis. Once initial host barrier systems have been breached by these pathogens, the innate immune system provides the next level of defense against these infectious agents. The innate immune response is characterized by the induction of proinflammatory cytokines, as well as increases in other accessory proteins that facilitate host recognition and elimination of the pathogens. The objective of the current study was to characterize the innate immune response during clinical mastitis elicited by these two important, yet less wellstudied, Gram-positive and Gram-negative organisms. The pro-inflammatory cytokine response and changes in the levels of the innate immune accessory recognition proteins, soluble CD14 (sCD14) and lipopolysaccharide (LPS)-binding protein (LBP), were studied. Decreased milk output, induction of a febrile response, and increased acute phase synthesis of LBP were all characteristic of the systemic response to intramammary infection with either organism. Infection with either bacteria similarly resulted in increased milk levels of IL-1β, IL-8, IL-10, IL-12, IFN-γ, TNF-α, sCD14, LBP, and the complement component, C5a. However, the duration of and/or maximal changes in the increased levels of these inflammatory markers were significantly different for several of the inflammatory parameters assayed. In particular, S. uberis infection was characterized by the sustained elevation of higher milk levels of IL-1β, IL-10, IL-12, IFN-γ, and C5a, relative to S. marcescens infection. Together, these data demonstrate the variability of the innate immune response to two distinct mastitis pathogens. cytokines / innate immunity / mastitis / Serratia / Streptococcus

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Molecular Epidemiology ofStreptococcus uberisIsolates from Dairy Cows with Mastitis

Cited by 94 publications

References 25 publications

Application of Streptococcus uberis Multilocus Sequence Typing: Analysis of the Population Structure Detected among Environmental and Bovine Isolates from New Zealand and the United Kingdom

Application of Streptococcus uberis Multilocus Sequence Typing: Analysis of the Population Structure Detected among Environmental and Bovine Isolates from New Zealand and the United Kingdom

Effect of mastitis and related-germ on milk yield and composition during naturally-occurring udder infections in dairy cows

Innate immune response to intramammary infection with Serratia marcescens and Streptococcus uberis

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