Molecular Epidemiology of mcr-1, blaKPC-2, and blaNDM-1 Harboring Clinically Isolated Escherichia coli from Pakistan

Bilal, Hazrat; Rehman, Tayyab; Khan, Muhammad Asif; Hameed, Fareeha; Jian, Zhang; Han, Jianxiong; Yang, Xingyuan

doi:10.2147/idr.s302687

Cited by 20 publications

(10 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of ESBL genes in commercial and rural poultry has been reported in previous studies, which are in line with our results ( Sarba et al., 2019 ). In clinical isolates, the presence of bla TEM was high compared to bla CTX-M-1, bla CTX-M-8, bla CTX-M-9, and bla CTX-M-15, which is different compared to previous studies ( Osuna et al., 2016 ; Rahman et al., 2016 ; Bilal et al., 2021 ).…”

Section: Discussioncontrasting

confidence: 85%

Occurrence of extended- spectrum β-lactamase harboring K. pneumoniae in various sources: a one health perspective

Yasmeen

Aslam

Fang

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

This study was designed to investigate the occurrence and dissemination of extended-spectrum β-lactamase (ESBL) harboring Klebsiella pneumoniae in various ecological niches under the one health approach. A total of 793 samples were collected from animals, humans, and the environment. The findings of the study revealed the occurrence of K. pneumoniae as follows: animals (11.6%), humans (8.4%), and associated environments (7.0%), respectively. A high occurrence rate of ESBL genes was found in animals compared to human and environmental isolates. A total of 18 distinct sequence types (STs) and 12 clonal complexes of K. pneumoniae were observed. Overall, six STs of K. pneumoniae were identified in commercial chickens, and three were found in rural poultry. The majority of K. pneumoniae STs found in this study were positive for blaSHV, while the positivity of other ESBL-encoding genes combinations was different in different STs. The high occurrence rate of ESBL-harboring K. pneumoniae found in animals as compared to other sources is alarming and has the potential to be disseminated to the associated environment and community.

show abstract

Section: Discussioncontrasting

confidence: 85%

Occurrence of extended- spectrum β-lactamase harboring K. pneumoniae in various sources: a one health perspective

Yasmeen

Aslam

Fang

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…No environmental or feed isolates evaluated show a co-harboring of carbapenemases and mcr genes. The dissemination of isolates that harbor mcr-1 and carbapenemases constitutes a public health issue, although these isolates have been reported in low frequency until now ( Dalmolin et al, 2019 ; Bilal et al, 2021 ). The increasing detection of isolates that co-harbor critically important ARGs emphasized the importance of prompt surveillance strategies to reduce the dissemination of these highly critical clones worldwide.…”

Section: Resultsmentioning

confidence: 99%

Genomic insights of mcr-1 harboring Escherichia coli by geographical region and a One-Health perspective

Calero-Cáceres

Rodríguez²,

Medina³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

The importance of the One Health concept in attempting to deal with the increasing levels of multidrug-resistant bacteria in both human and animal health is a challenge for the scientific community, policymakers, and the industry. The discovery of the plasmid-borne mobile colistin resistance (mcr) in 2015 poses a significant threat because of the ability of these plasmids to move between different bacterial species through horizontal gene transfer. In light of these findings, the World Health Organization (WHO) recommends that countries implement surveillance strategies to detect the presence of plasmid-mediated colistin-resistant microorganisms and take suitable measures to control and prevent their dissemination. Seven years later, ten different variants of the mcr gene (mcr-1 to mcr-10) have been detected worldwide in bacteria isolated from humans, animals, foods, the environment, and farms. However, the possible transmission mechanisms of the mcr gene among isolates from different geographical origins and sources are largely unknown. This article presents an analysis of whole-genome sequences of Escherichia coli that harbor mcr-1 gene from different origins (human, animal, food, or environment) and geographical location, to identify specific patterns related to virulence genes, plasmid content and antibiotic resistance genes, as well as their phylogeny and their distribution with their origin. In general, E. coli isolates that harbor mcr-1 showed a wide plethora of ARGs. Regarding the plasmid content, the highest concentration of plasmids was found in animal samples. In turn, Asia was the continent that led with the largest diversity and occurrence of these plasmids. Finally, about virulence genes, terC, gad, and traT represent the most frequent virulence genes detected. These findings highlight the relevance of analyzing the environmental settings as an integrative part of the surveillance programs to understand the origins and dissemination of antimicrobial resistance.

show abstract

“…Interestingly, all these resistant isolates were harbouring plasmid-mediated gene, mcr-1 indicating its ability of fast dissemination and horizontal transfer. Previous reports from Pakistan have already detected the mcr-1 gene from clinical, cattle, wild birds, poultry and the environment (Ali et al, 2021;Bilal et al, 2021;Mohsin et al, 2017Mohsin et al, , 2019. On top of this, the co-occurrence of ESBL and colistin is limiting the option for treatment in near future presenting a huge challenge for healthcare community.…”

Section: Discussionmentioning

confidence: 99%

Incidence and molecular characterization of ESBL-producing and colistin-resistant Escherichia coli isolates recovered from healthy food-producing animals in Pakistan

Shafiq

Rahman

Bilal

et al. 2022

Journal of Applied Microbiology

Self Cite

View full text Add to dashboard Cite

Objectives: To investigate the occurrence and molecular features of ESBL-producing and colistin-resistant Escherichia coli isolates recovered from healthy food-producing animals in Pakistan. Methods: A total of 153 E. coli isolates were recovered from 250 faecal samples collected from livestock and poultry. The antibiotic susceptibility, resistant determinants and mobile genetic elements were determined for all the isolates. The clonal relatedness was analysed by MLST. Plasmids harbouring, localization and transferability of mcr-1 gene were carried out by Southern hybridization, S1-PFGE and transconjugation.Results: Out of 153 E. coli strains, 49.01% isolates were ESBLs producers, whereas 18.95% were resistant to colistin and 84.31% of the isolates. Multidrug resistance was found in 84% of the isolates. The ESBL-producing E. coli in buffaloes, cattle, sheep, goat and broilers faecal samples were 60%, 74%, 54%, 50% and 68%, respectively. Among the ESBLs genes, bla CTX-M was the most prevalent group detected in 98.66%, while only mcr-1 of the colistin-resistant genes could be PCR amplified in 29 isolates. The common MGEs found were ISECP1 (35.13%), ISCR1 (33.78%), ISApl1 (20.27%) and Inti1 (58.10%). The most predominant Inc. types found were IncFIB 46.66%, followed by IncFIA 30.66%, IncFIC 26.66%, IncFrepB 26.66%, IncHI2 26.66%, IncP 22.66% and IncX4 21.33%. The most frequent sequence type detected was ST58.Southern blot and S1-PFGE confirmed the plasmid harbouring of mcr-1 gene. Conclusion:The co-occurrence of mcr-1 and ESBLs-encoding genes, along with MGEs in E. coli from healthy food animals in Pakistan, is a major concern.

show abstract

Molecular Epidemiology of mcr-1, blaKPC-2, and blaNDM-1 Harboring Clinically Isolated Escherichia coli from Pakistan

Cited by 20 publications

References 57 publications

Occurrence of extended- spectrum β-lactamase harboring K. pneumoniae in various sources: a one health perspective

Occurrence of extended- spectrum β-lactamase harboring K. pneumoniae in various sources: a one health perspective

Genomic insights of mcr-1 harboring Escherichia coli by geographical region and a One-Health perspective

Incidence and molecular characterization of ESBL-producing and colistin-resistant Escherichia coli isolates recovered from healthy food-producing animals in Pakistan

Contact Info

Product

Resources

About